Restricting the conformational heterogeneity of RNA by specific incorporation of 8-bromoguanosine.

Restricting the conformational heterogeneity of RNA by specific incorporation of 8-bromoguanosine. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Restricting the conformational heterogeneity of RNA by specific incorporation of 8-bromoguanosine. Abstract Text:

David J Proctor,Elzbieta Kierzek,Ryszard Kierzek,Philip C Bevilacqua,

In an effort to reduce the conformational heterogeneity of RNA, the modified nucleobase 8-bromoguanosine (8BrG) was introduced into oligonucleotides having the hairpin tetraloop motif YNMG (Y = U or C and M = C or A). Purine nucleobases with bromine at position eight are known to preferentially adopt the syn conformation as nucleosides. The hairpin tetraloop motif YNMG was chosen as a model system because it has a syn guanosine at position four of the loop that is essential for thermodynamic stability. Thermodynamic and structural characterization of modified oligonucleotides with the hairpin sequences UUCG, CGCG, and CGAG by UV-melting and NMR spectroscopy revealed that 8BrG substitution has a small effect upon the hairpin conformation, while the duplex conformation is strongly destabilized (DeltaDeltaG degrees 37 approximately +4.7 kcal mol-1), thus inhibiting dimerization. These results support a model in which 8BrG substitution shifts the hairpin-duplex equilibrium constant toward the hairpin conformation by destabilizing the duplex. This methodology should be useful for limiting conformational heterogeneity in large RNAs, with potential applications in structural biology and enzymology.

Restricting the conformational heterogeneity of RNA by specific incorporation of 8-bromoguanosine. Publishing Authors By Initials

DJ Proctor,E Kierzek,R Kierzek,PC Bevilacqua,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids: rna research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids: rna research

PUBMED ID PMID:

MEDLINE DATE:

Restricting the conformational heterogeneity of RNA by specific incorporation of 8-bromoguanosine. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 125

Page Numbers: 2390-1

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 5

MONTH: Mar

YEAR: 2003

Restricting the conformational heterogeneity of RNA by specific incorporation of 8-bromoguanosine. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

Restricting the conformational heterogeneity of RNA by specific incorporation of 8-bromoguanosine. Keywords Mesh Terms:

KEYWORDS: RNA

MESH TERMS: chemistry

Chemical & Substance for Abstract: Restricting the conformational heterogeneity of RNA by specific incorporation of 8-bromoguanosine. Information

Substance Name: RNA

Registry Number: 63231-63-0

Grant and Affiliation Information for Restricting the conformational heterogeneity of RNA by specific incorporation of 8-bromoguanosine.

AFFILIATION: Department of Chemistry, The Pennsylvania State University, University Park, PA 16802, USA.

Country: United States

AGENCY: United States FIC

GRANT: 1R03 TW 1068

ACRONYM: TW

MEDLINETA: J Am Chem Soc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Restricting the conformational heterogeneity of RNA by specific incorporation of 8-bromoguanosine Related Publications

• 5'-triphosphate-dependent activation of PKR by RNAs with short stem-loops.
• Acid phosphatase and cathepsin D are active expressed enzymes in the placenta of the cat.
• Bcl-2 and bax expressions in pre-term, term and post-term placentas.
• Endoscopic management of symptomatic choledocholithiasis in pregnancy without the use of radiations.
• Imaging of the charcot foot.
• Mechanistic characterization of the HDV genomic ribozyme: a mutant of the C41 motif provides insight into the positioning and thermodynamic linkage of metal ions and protons.
• Mechanistic characterization of the HDV genomic ribozyme: the cleavage site base pair plays a structural role in facilitating catalysis.
• Nucleoside modifications modulate activation of the protein kinase PKR in an RNA structure-specific manner.
• Reconstructing the Genealogy of a BRCA1 Founder Mutation by Phylogenetic Analysis.
• Regulation of gene expression in mouse trophoblast cells by interferon-gamma.
• Restricting the conformational heterogeneity of RNA by specific incorporation of 8-bromoguanosine.
• Signaling Molecules Involved in IFN-gamma-Inducible Nitric Oxide Synthase Expression in the Mouse Trophoblast.
• Simple method for determining nucleobase pK(a) values by indirect labeling and demonstration of a pK(a) of neutrality in dsDNA.
• The diagnosis of charcot foot.
• The influence of nutritional status on skin anergy in experimental cancer.
• The proteins in unheated culture filtrates of human tubercle bacilli; determination of serological properties.
• Wild-type is the optimal sequence of the HDV ribozyme under cotranscriptional conditions.
• [Burnout in healthcare workers of a university teaching hospital in Rome, Italy: a cross-sectional study.]
• [Cancer of the tongue; case report.]
• [Comments on cancer of the oral cavity.]
• [Experimental histopathological studies of the toxicity of indirect anticoagulants.]
• [Not Available.]
• [Not Available.]