Respiratory carcinoma cell lines. MUC genes and glycoconjugates.

Respiratory carcinoma cell lines. MUC genes and glycoconjugates. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Respiratory carcinoma cell lines. MUC genes and glycoconjugates. Abstract Text:

J T Berger,J A Voynow,K W Peters,M C Rose,

Lung carcinoma cell lines are being used in many laboratories to study various airway epithelial functions, including mucin gene expression. To identify model systems for investigating regulation of MUC5/5AC gene expression and secretion of MUC5/5AC mucins in airway epithelial cells, we evaluated the expression of several mucin genes in six carcinoma cell lines of respiratory tract origin. RNA was extracted from A549, Calu-3, NCI H292, Calu-6, RPMI 2650, and A-427 cells; MUC1, MUC2, MUC4, MUC5/5AC, and MUC5B messenger RNA (mRNA) expression was determined. By Northern analyses, all cell lines expressed MUC1 mRNA, whereas MUC2 mRNA was not detectable in any of the cell lines. RPMI 2650 cell lines expressed only MUC1 mRNA. NCI-H292 cells expressed MUC4 and low levels of MUC5/5AC mRNA. Calu-3 and A549 cells expressed MUC5/5AC mRNA; A549 cells also expressed MUC5B mRNA. Glycoconjugates secreted by lung carcinoma cells were also examined. By wheat germ lectin analysis, Calu-3, H292, and A549 cells secreted high molecular weight glycoproteins having N-acetylglucosamine and/or sialic acid moieties. Western blot analyses with an anti-MUC5:TR-3A antibody demonstrated that Calu-3 and A549 cells secreted MUC5/5AC mucins. All six carcinoma cell lines secreted large, radiolabeled, sulfated macromolecules; the majority were proteoglycans that were digested by hyaluronidase. However, Calu-3 cells also secreted sulfated high molecular-weight glycoproteins that were immunoprecipitated by anti-MUC5:TR-3A antibody. These studies demonstrated that Calu-3 and A549 cell lines expressed high and moderate amounts of MUC5/5AC mRNA and MUC5/5AC mucins, whereas H292 cells expressed lesser amounts. These cell lines should prove useful for studies of MUC5/5AC gene expression and MUC5/5AC biosynthesis, trafficking, and secretions in airway epithelial cells.

Respiratory carcinoma cell lines. MUC genes and glycoconjugates. Publishing Authors By Initials

JT Berger,JA Voynow,KW Peters,MC Rose,

For similar cells: cells, cultured: tumor cells, cultured research abstracts see: cells: cells, cultured: tumor cells, cultured research

PUBMED ID PMID:

MEDLINE DATE:

Respiratory carcinoma cell lines. MUC genes and glycoconjugates. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: American journal of respiratory cell and molecular

VOLUME: 20

Page Numbers: 500-10

Journal Abbreviation: Am. J. Respir. Cell Mol. Biol.

ISSN: 1044-1549

DAY: 29

MONTH: Mar

YEAR: 1999

Respiratory carcinoma cell lines. MUC genes and glycoconjugates. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8917225

Respiratory carcinoma cell lines. MUC genes and glycoconjugates. Keywords Mesh Terms:

KEYWORDS: Tumor Cells, Cultured

MESH TERMS: metabolism

Chemical & Substance for Abstract: Respiratory carcinoma cell lines. MUC genes and glycoconjugates. Information

Substance Name: RNA, Messenger

Registry Number: 0

Grant and Affiliation Information for Respiratory carcinoma cell lines. MUC genes and glycoconjugates.

AFFILIATION: Center for Molecular Mechanisms of Diseases, Children's Research Institute, Children's National Medical Center, Washington, DC 20010-2970, USA.

Country: UNITED STATES

AGENCY: United States NHLBI

GRANT: R29 HL50694

ACRONYM: HL

MEDLINETA: Am J Respir Cell Mol Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Respiratory carcinoma cell lines MUC genes and glycoconjugates Related Publications

• A Naïve Artist of St IVES.
• A general solution for the time delay introduced by a low-pass Butterworth digital filter: An application to musculoskeletal modeling.
• Autoimmune thyroid diseases.
• Characterization of endotoxin and mouse allergen exposures in mouse facilities and research laboratories.
• Cloning and analysis of cDNA encoding a major airway glycoprotein, human tracheobronchial mucin (MUC5).
• Combined thyroidectomy and renal denervation suppress renin expression and secretion in fetal sheep.
• Coxsackievirus-induced myocarditis in mice: a model of autoimmune disease for studying immunotoxicity.
• Do longevity mutants always show trade-offs?
• Editor's Note.
• Effect of leucine or ketoleucine on nitrogen metabolism in postoperative patients receiving energy substrate.
• Fipronil induces CYP isoforms and cytotoxicity in human hepatocytes.
• Metabolism of endosulfan-alpha by human liver microsomes and its utility as a simultaneous in vitro probe for CYP2B6 and CYP3A4.
• Muc-5/5ac mucin messenger RNA and protein expression is a marker of goblet cell metaplasia in murine airways.
• Mucin gene expression (MUC1, MUC2, and MUC5/5AC) in nasal epithelial cells of cystic fibrosis, allergic rhinitis, and normal individuals.
• Neuroactive steroids, negative affect, and nicotine dependence severity in male smokers.
• Ontogeny and effects of hypothalamic pituitary disconnection on formation of inositol trisphosphate in fetal sheep pituitary cells.
• Paleopathology of the commoners at Tell Amarna, Egypt, Akhenaten's capital city.
• Parental monitoring moderates the importance of genetic and environmental influences on adolescent smoking.
• Predictors of parenting behavior trajectories among families of young adolescents with and without spina bifida.
• Regulation of mucin genes in chronic inflammatory airway diseases.
• Reply.
• Respiratory carcinoma cell lines. MUC genes and glycoconjugates.
• Role of transcription factor Kar4 in regulating downstream events in the Saccharomyces cerevisiae pheromone response pathway.
• Temporal analysis of goblet cells and mucin gene expression in murine models of allergic asthma.
• The deafness of goya (1746-1828).
• The impact of manual handling training on work place injuries: a 14 year audit.
• The importance of cytochrome P450 2B6 in the human metabolism of environmental chemicals.
• Van Gogh's Madness.
• Waterborne cryptosporidiosis: current status.
• [In Process Citation]