Resilience of Rhodobacter sphaeroides cytochrome bc1 to heme c1 ligation changes.

Resilience of Rhodobacter sphaeroides cytochrome bc1 to heme c1 ligation changes. Research Abstract Details 

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Resilience of Rhodobacter sphaeroides cytochrome bc1 to heme c1 ligation changes. Abstract Text:

Haibo Zhang,Artur Osyczka,Christopher C Moser,P Leslie Dutton,

Typically, c hemes are bound to the protein through two thioether bonds to cysteines and two axial ligands to the heme iron. In high-potential class I c-type cytochromes, these axial ligands are commonly His-Met. A change in this methionine axial ligand is often correlated with a dramatic drop in the heme redox potential and loss of function. Here we describe a bacterial cytochrome c with an unusual tolerance to the alternations in the heme ligation pattern. Substitution of the heme ligating methionine (M185) in cytochrome c1 of the Rhodobacter sphaeroides cytochrome bc1 complex with Lys and Leu lowers the redox midpoint potential but not enough to prevent physiologically competent electron transfer in these fully functional variants. Only when Met-185 is replaced with His is the drop in the redox potential sufficiently large to cause cytochrome bc1 electron transfer chain failure. Functional mutants preserve the structural integrity of the heme crevice: only the nonfunctional His variant allows carbon monoxide to bind to reduced heme, indicating a significant opening of the heme environment. This range of cytochrome c1 ligand mutants exposes both the relative resilience to sixth axial ligand change and the ultimate thermodynamic limits of operation of the cofactor chains in cytochrome bc1.

Resilience of Rhodobacter sphaeroides cytochrome bc1 to heme c1 ligation changes. Publishing Authors By Initials

H Zhang,A Osyczka,CC Moser,PL Dutton,

For similar investigative techniques: chemistry, analytical: spectrum analysis research abstracts see: investigative techniques: chemistry, analytical: spectrum analysis research

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MEDLINE DATE:

Resilience of Rhodobacter sphaeroides cytochrome bc1 to heme c1 ligation changes. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Biochemistry

VOLUME: 45

Page Numbers: 14247-55

Journal Abbreviation: Biochemistry

ISSN: 0006-2960

DAY: 5

MONTH: Dec

YEAR: 2006

Resilience of Rhodobacter sphaeroides cytochrome bc1 to heme c1 ligation changes. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370623

Resilience of Rhodobacter sphaeroides cytochrome bc1 to heme c1 ligation changes. Keywords Mesh Terms:

KEYWORDS: Spectrum Analysis

MESH TERMS: genetics

Chemical & Substance for Abstract: Resilience of Rhodobacter sphaeroides cytochrome bc1 to heme c1 ligation changes. Information

Substance Name: Electron Transport Complex III

Registry Number: EC 1.10.2.2

Grant and Affiliation Information for Resilience of Rhodobacter sphaeroides cytochrome bc1 to heme c1 ligation changes.

AFFILIATION: The Johnson Research Foundation, Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6059, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM27309

ACRONYM: GM

MEDLINETA: Biochemistry

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