Resident dendritic cells are the predominant TNF-secreting cell in early renal ischemia-reperfusion injury.

Resident dendritic cells are the predominant TNF-secreting cell in early renal ischemia-reperfusion injury. Research Abstract Details 

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Resident dendritic cells are the predominant TNF-secreting cell in early renal ischemia-reperfusion injury. Abstract Text:

Renal ischemia-reperfusion injury (IRI) rapidly induces production of inflammatory mediators including, and in particular, tumor necrosis factor (TNF). Possible sources include resident parenchymal and bone marrow-derived cells as well as recruited leukocytes. Cell suspensions from kidneys subjected to IRI were examined by cell separation followed by in vitro culture and enzyme-linked immunosorbent assay (ELISA), immunoperoxidase and immunofluorescence microscopy, and multicolor flow cytometry to determine the contribution of dendritic cells (DCs) to early production of TNF and other inflammatory mediators. Secretion of TNF, interleukin (IL-6), monocyte chemoattractant protein-1 (MCP-1), and regulated on activation normal T cell expressed and secreted (RANTES) was increased in cell suspensions from IRI compared with control kidneys and was higher in DC-enriched preparations. Immunostaining identified TNF(+ve) cells that coexpressed the DC marker CD11c. Flow cytometry of bone marrow-derived (CD45(+ve)) cell populations at 24 h post-IRI demonstrated that F4/80(+ve)/CD11c(+ve) DCs remained proportionately stable and exhibit higher levels of DC maturation markers, whereas the proportion of F4/80(-ve) DCs, monocytes, neutrophils, and T cells increased. Intracellular staining for TNF confirmed that F4/80(+ve) DCs were the predominant TNF(+ve) cell and expressed higher levels than other TNF(+ve) cells. In vivo depletion of DCs from the kidney substantially attenuated TNF secretion by total and CD45(+ve) cells following IRI. The results uncover a role for resident F4/80(+ve) DCs as the predominant secretors of TNF within 24 h of IRI.

Resident dendritic cells are the predominant TNF-secreting cell in early renal ischemia-reperfusion injury. Publishing Authors By Initials

For similar peptides: intercellular signaling peptides and proteins: cytokines: monokines: tumor necrosis factor-alpha research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: monokines: tumor necrosis factor-alpha research

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Resident dendritic cells are the predominant TNF-secreting cell in early renal ischemia-reperfusion injury. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Kidney international

VOLUME: 71

Page Numbers: 619-28

Journal Abbreviation: Kidney Int.

ISSN: 0085-2538

DAY: 21

MONTH: 02

YEAR: 2007

Resident dendritic cells are the predominant TNF-secreting cell in early renal ischemia-reperfusion injury. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 323470

Resident dendritic cells are the predominant TNF-secreting cell in early renal ischemia-reperfusion injury. Keywords Mesh Terms:

KEYWORDS: Tumor Necrosis Factor-alpha

MESH TERMS: secretion

Chemical & Substance for Abstract: Resident dendritic cells are the predominant TNF-secreting cell in early renal ischemia-reperfusion injury. Information

Substance Name: Tumor Necrosis Factor-alpha

Registry Number: 0

Grant and Affiliation Information for Resident dendritic cells are the predominant TNF-secreting cell in early renal ischemia-reperfusion injury.

AFFILIATION: Division of Nephrology and Hypertension, Department of Medicine, Mayo Clinic College of Medicine, Rochester, Minnesota, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: T32DK07013

ACRONYM: DK

MEDLINETA: Kidney Int

REFSOURCE: Kidney Int. 2007 Apr;71(7):604-5

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