Rescue of Akabane virus (family Bunyaviridae) entirely from cloned cDNAs by using RNA polymerase I.

Rescue of Akabane virus (family Bunyaviridae) entirely from cloned cDNAs by using RNA polymerase I. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Rescue of Akabane virus (family Bunyaviridae) entirely from cloned cDNAs by using RNA polymerase I. Abstract Text:

Yohsuke Ogawa,Keita Sugiura,Kentaro Kato,Yukinobu Tohya,Hiroomi Akashi,Yohsuke Ogawa,Keita Sugiura,Kentaro Kato,Yukinobu Tohya,Hiroomi Akashi,Yohsuke Ogawa,Keita Sugiura,Kentaro Kato,Yukinobu Tohya,Hiroomi Akashi,

Reverse-genetic systems are often used to study different aspects of the viral life cycle. To date, three rescue systems have been developed for the family Bunyaviridae. These systems use T7 RNA polymerase, which is generally used in rescue systems for Mononegavirales. In the present study, we describe a rescue system for Akabane virus (family Bunyaviridae) that uses cDNAs and RNA polymerase I instead of T7 RNA polymerase. The utility of this system was demonstrated by the generation of a mutant with a deletion of the non-structural protein (NSs) on the S RNA segment. These results offer a new option for bunyavirus rescue.

Rescue of Akabane virus (family Bunyaviridae) entirely from cloned cDNAs by using RNA polymerase I. Publishing Authors By Initials

Y Ogawa,K Sugiura,K Kato,Y Tohya,H Akashi,Y Ogawa,K Sugiura,K Kato,Y Tohya,H Akashi,Y Ogawa,K Sugiura,K Kato,Y Tohya,H Akashi,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Rescue of Akabane virus (family Bunyaviridae) entirely from cloned cDNAs by using RNA polymerase I. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of general virology

VOLUME: 88

Page Numbers: 3385-90

Journal Abbreviation: J. Gen. Virol.

ISSN: 0022-1317

DAY: 20

MONTH: Dec

YEAR: 2007

Rescue of Akabane virus (family Bunyaviridae) entirely from cloned cDNAs by using RNA polymerase I. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 77340

Rescue of Akabane virus (family Bunyaviridae) entirely from cloned cDNAs by using RNA polymerase I. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Rescue of Akabane virus (family Bunyaviridae) entirely from cloned cDNAs by using RNA polymerase I. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Rescue of Akabane virus (family Bunyaviridae) entirely from cloned cDNAs by using RNA polymerase I.

AFFILIATION: Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Gen Virol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Rescue of Akabane virus family Bunyaviridae entirely from cloned cDNAs by using RNA polymerase I Related Publications

• A case of an embryo transfer calf infected with bovine leukemia virus from the recipient cow.
• A novel biomaterial: poly(dimethylsiloxane)-polyamide multiblock copolymer I. Synthesis and evaluation of blood compatibility.
• An accidental exposure by a medical linear accelerator under construction.
• Cell adhesion and proliferation on poly(N-vinylacetamide) hydrogels and double network approaches for changing cellular affinities.
• Cell-adhesive and blood-coagulant properties of ultrathin poly(methyl methacrylate) stereocomplex films.
• Cell-compatible properties of calcium carbonates and hydroxyapatite deposited on ultrathin poly(vinyl alcohol)-coated polyethylene films.
• Characterization of temperature-sensitive Akabane virus mutants and their roles in attenuation.
• Coil embolization of splenic artery aneurysm with preservation of the parent artery using a neck remodeling technique.
• Collection of mobilized peripheral blood stem cells from a donor with severe iron deficient anemia.
• Comparison of Akabane virus isolated from sentinel cattle in Japan.
• Hematopoietic developmental pathways: on cellular basis.
• Hsp25 regulates the expression of p21(Waf1/Cip1/Sdi1) through multiple mechanisms.
• Hydroxyapatite deposition by alternating soaking technique on poly(vinyl alcohol)-coated polyethylene films.
• IL-1 induces expression of p21(WAF1) independently of p53 in high-passage human embryonic fibroblasts WI38.
• INFLUENCES OF ANTICANCER AGENTS ON THE METABOLISM OF DELTA-AMINOLEVULINIC ACID IN MORMAL AND TUMOR-BEARING MICE.
• IgG responses to intranasal immunization with cholera-toxin-immobilized polymeric nanospheres in mice.
• Inorganic-organic polymer hybrid scaffold for tissue engineering--II: partial enzymatic degradation of hydroxyapatite-chitosan hybrid.
• MR imaging of an atypical vaginal leiomyoma.
• Novel functional polymers: poly(dimethylsiloxane)-polyamide multiblock copolymer. V. The interaction between biomolecules and the surface of aramid-silicone resins.
• Physical adsorption of human thrombomodulin (ART-123) onto polymeric biomaterials for developing an antithrombogenic blood-contacting material.
• Polymer-phloridzin conjugates as an anti-diabetic drug that Inhibits glucose absorption through the Na(+)/glucose cotransporter (SGLT1) in the small intestine.
• Programmed proteome response for drought avoidance/tolerance in the root of a c3 xerophyte (wild watermelon) under water deficits.
• Quick-forming hydroxyapatite/agarose gel composites induce bone regeneration.
• Rescue of Akabane virus (family Bunyaviridae) entirely from cloned cDNAs by using RNA polymerase I.
• Study of cellular responses to polymeric biomaterials using the differential display method.
• Synthesis and properties of coumaric acid derivative homo-polymers.
• The developmental program of human dendritic cells is operated independently of conventional myeloid and lymphoid pathways.
• The order of expression of transcription factors directs hierarchical specification of hematopoietic lineages.
• [Macrolide and macrolide-related antibacterial agents]
• [Methodology in educational research. 8. Methods of recording in continued surveys]