Requirements for the localization of nesprin-3 at the nuclear envelope and its interaction with plectin.

Requirements for the localization of nesprin-3 at the nuclear envelope and its interaction with plectin. Research Abstract Details 

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Requirements for the localization of nesprin-3 at the nuclear envelope and its interaction with plectin. Abstract Text:

Mirjam Ketema,Kevin Wilhelmsen,Ingrid Kuikman,Hans Janssen,Didier Hodzic,Arnoud Sonnenberg,

The outer nuclear membrane proteins nesprin-1 and nesprin-2 are retained at the nuclear envelope through an interaction of their klarsicht/ANC-1/syne homology (KASH) domain with Sun proteins present at the inner nuclear membrane. We investigated the requirements for the localization of nesprin-3alpha at the outer nuclear membrane and show that the mechanism by which its localization is mediated is similar to that reported for the localization of nesprin-1 and nesprin-2: the last four amino acids of the nesprin-3alpha KASH domain are essential for its interaction with Sun1 and Sun2. Moreover, deletion of these amino acids or knockdown of the Sun proteins results in a redistribution of nesprin-3alpha away from the nuclear envelope and into the endoplasmic reticulum (ER), where it becomes colocalized with the cytoskeletal crosslinker protein plectin. Both nesprin-3alpha and plectin can form dimers, and dimerization of plectin is required for its interaction with nesprin-3alpha at the nuclear envelope, which is mediated by its N-terminal actin-binding domain. Additionally, overexpression of the plectin actin-binding domain stabilizes the actin cytoskeleton and prevents the recruitment of endogenous plectin to the nuclear envelope. Our studies support a model in which the actin cytoskeleton influences the binding of plectin dimers to dimers of nesprin-3alpha, which in turn are retained at the nuclear envelope through an interaction with Sun proteins.

Requirements for the localization of nesprin-3 at the nuclear envelope and its interaction with plectin. Publishing Authors By Initials

M Ketema,K Wilhelmsen,I Kuikman,H Janssen,D Hodzic,A Sonnenberg,

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Requirements for the localization of nesprin-3 at the nuclear envelope and its interaction with plectin. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of cell science

VOLUME: 120

Page Numbers: 3384-94

Journal Abbreviation: J. Cell. Sci.

ISSN: 0021-9533

DAY: 1

MONTH: Oct

YEAR: 2007

Requirements for the localization of nesprin-3 at the nuclear envelope and its interaction with plectin. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Requirements for the localization of nesprin-3 at the nuclear envelope and its interaction with plectin.

AFFILIATION: Division of Cell Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.

Country: England

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MEDLINETA: J Cell Sci

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