Requirements for polyadenylation at the 3' end of LINE-1 elements.

Requirements for polyadenylation at the 3' end of LINE-1 elements. Research Abstract Details 

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Requirements for polyadenylation at the 3' end of LINE-1 elements. Abstract Text:

Victoria P Belancio,Megan Whelton,Prescott Deininger,

LINE-1 (L1) is the only active, autonomous, non-LTR, human retroelement. There are about 5x10(5) L1 copies in the human genome, the majority of which are truncated at their 5' ends. Both truncated and full-length L1 insertions contain a polyadenylation (polyA) signal at their 3' ends. A typical polyA site consists of the three main cis-acting elements: a conserved hexamer, cleavage site, and a GU-rich downstream region. A newly inserted L1 copy contains the conserved AATAAA hexamer at the end of its sequence. However, the GU-rich downstream region has to be provided by the neighboring genomic sequences and therefore it would vary for every L1 copy. Using northern blot analysis of transiently transfected L1 expression vectors we demonstrate that L1 element contain sequence that allow efficient polyadenylation at the L1 3' end upon retrotransposition into a new genomic location independent of the base composition downstream of the insertion site. The strategy of polyadenylation at the 3' end of L1 parallels the approach the element employs at its 5'UTR by having an unusual internal polymerase II promoter, making new insertions less dependent on the properties of the flanking sequences at the new locus.

Requirements for polyadenylation at the 3' end of LINE-1 elements. Publishing Authors By Initials

VP Belancio,M Whelton,P Deininger,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, messenger research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, messenger research

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Requirements for polyadenylation at the 3' end of LINE-1 elements. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Gene

VOLUME: 390

Page Numbers: 98-107

Journal Abbreviation: Gene

ISSN: 0378-1119

DAY: 18

MONTH: 08

YEAR: 2006

Requirements for polyadenylation at the 3' end of LINE-1 elements. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7706761

Requirements for polyadenylation at the 3' end of LINE-1 elements. Keywords Mesh Terms:

KEYWORDS: RNA, Messenger

MESH TERMS: genetics

Chemical & Substance for Abstract: Requirements for polyadenylation at the 3' end of LINE-1 elements. Information

Substance Name: RNA, Messenger

Registry Number: 0

Grant and Affiliation Information for Requirements for polyadenylation at the 3' end of LINE-1 elements.

AFFILIATION: Tulane Cancer Center, SL66, and Department of Epidemiology, Tulane University Health Sciences Center, 1430 Tulane Ave., New Orleans, LA 70112, United States.

Country: Netherlands

AGENCY: United States NIGMS

GRANT: R01GM45668

ACRONYM: GM

MEDLINETA: Gene

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