Requirement of glycosylphosphatidylinositol anchor of Cripto-1 for trans activity as a Nodal co-receptor.

Requirement of glycosylphosphatidylinositol anchor of Cripto-1 for trans activity as a Nodal co-receptor. Research Abstract Details 

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Requirement of glycosylphosphatidylinositol anchor of Cripto-1 for trans activity as a Nodal co-receptor. Abstract Text:

Kazuhide Watanabe,Shin Hamada,Caterina Bianco,Mario Mancino,Tadahiro Nagaoka,Monica Gonzales,Veronique Bailly,Luigi Strizzi,David S Salomon,Kazuhide Watanabe,Shin Hamada,Caterina Bianco,Mario Mancino,Tadahiro Nagaoka,Monica Gonzales,Veronique Bailly,Luigi Strizzi,David S Salomon,Kazuhide Watanabe,Shin Hamada,Caterina Bianco,Mario Mancino,Tadahiro Nagaoka,Monica Gonzales,Veronique Bailly,Luigi Strizzi,David S Salomon,

Cripto-1 (CR-1) has an indispensable role as a Nodal co-receptor for patterning of body axis in embryonic development. CR-1 is reported to have a paracrine activity as a Nodal co-receptor, although CR-1 is primarily produced as a glycosylphosphatidylinositol (GPI)-anchored membrane protein. Regulation of cis and trans function of CR-1 should be important to establish the precise body patterning. However, the mechanism by which GPI-anchored CR-1 can act in trans is not well known. Here we confirmed the paracrine activity of CR-1 by fluorescent cell-labeling and immunofluorescent staining. We generated COOH-terminal-truncated soluble forms of CR-1 based on the attachment site for the GPI moiety (omega-site), which we identified in the present study. GPI-anchored CR-1 has a significantly higher activity than COOH-terminal-truncated soluble forms to induce Nodal signal in trans as well as in cis. Moreover, transmembrane forms of CR-1 partially retained their ability to induce Nodal signaling only when type I receptor Activin-like kinase 4 was overexpressed. NTERA2/D1 cells, which express endogenous CR-1, lost the cell-surface expression of CR-1 after phosphatidylinositol-phospholipase C treatment and became refractory to stimulation of Nodal. These observations suggest that GPI attachment of CR-1 is required for the paracrine activity as a Nodal co-receptor.

Requirement of glycosylphosphatidylinositol anchor of Cripto-1 for trans activity as a Nodal co-receptor. Publishing Authors By Initials

K Watanabe,S Hamada,C Bianco,M Mancino,T Nagaoka,M Gonzales,V Bailly,L Strizzi,DS Salomon,K Watanabe,S Hamada,C Bianco,M Mancino,T Nagaoka,M Gonzales,V Bailly,L Strizzi,DS Salomon,K Watanabe,S Hamada,C Bianco,M Mancino,T Nagaoka,M Gonzales,V Bailly,L Strizzi,DS Salomon,

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Requirement of glycosylphosphatidylinositol anchor of Cripto-1 for trans activity as a Nodal co-receptor. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Intr

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 35772-86

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 9

MONTH: 10

YEAR: 2007

Requirement of glycosylphosphatidylinositol anchor of Cripto-1 for trans activity as a Nodal co-receptor. Information

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LANGUAGE: eng

NlmUniqueID: 2985121

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Grant and Affiliation Information for Requirement of glycosylphosphatidylinositol anchor of Cripto-1 for trans activity as a Nodal co-receptor.

AFFILIATION: Tumor Growth Factor Section, Mammary Biology and Tumorigenesis Laboratory, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA.

Country: United States

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MEDLINETA: J Biol Chem

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