Requirement of different subdomains of calpastatin for calpain inhibition and for binding to calmodulin-like domains.

Requirement of different subdomains of calpastatin for calpain inhibition and for binding to calmodulin-like domains. Research Abstract Details 

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Requirement of different subdomains of calpastatin for calpain inhibition and for binding to calmodulin-like domains. Abstract Text:

H Ma,H Q Yang,E Takano,W J Lee,M Hatanaka,M Maki,

Calpain requires Ca2+ for both proteolysis of its substrates and interaction with its endogenous inhibitor, calpastatin. The mechanism of inhibition of calpain by calpastatin has remained unsolved, although Nishimura and Goll [J. Biol. Chem. 266, 11842-11850 (1991)] reported that autolyzed calpain fragments containing calmodulin-like domains (CaMLDs) bound to an immobilized calpastatin column. We investigated the correlation between CaMLD-binding and calpain inhibition using immobilized columns of gene-engineered CaMLDs derived from the human mu-calpain large subunit and various recombinant calpastatin mutants. Among the four internally repetitive inhibitory domains of calpastatin, each having conserved regions A, B, and C, only domains 1 and 4 showed the binding activity. The region B deletion mutant of domain 1, retaining the CaMLD-binding ability, no longer had the calpain inhibition activity, and became susceptible to proteolysis. In contrast, a synthetic oligopeptide of region B with moderate calpain inhibition activity did not bind to the column. Domain 3 acquired the binding ability on substitution of region A with that of domain 1. These results suggest that calpain inhibition and binding to the CaMLDs are not correlated or mediated by different subdomains of calpastatin.

Requirement of different subdomains of calpastatin for calpain inhibition and for binding to calmodulin-like domains. Publishing Authors By Initials

H Ma,HQ Yang,E Takano,WJ Lee,M Hatanaka,M Maki,

For similar organic chemicals: amides: sulfonamides research abstracts see: organic chemicals: amides: sulfonamides research

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Requirement of different subdomains of calpastatin for calpain inhibition and for binding to calmodulin-like domains. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 113

Page Numbers: 591-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1993

Requirement of different subdomains of calpastatin for calpain inhibition and for binding to calmodulin-like domains. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Requirement of different subdomains of calpastatin for calpain inhibition and for binding to calmodulin-like domains. Keywords Mesh Terms:

KEYWORDS: Sulfonamides

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Requirement of different subdomains of calpastatin for calpain inhibition and for binding to calmodulin-like domains. Information

Substance Name: Calpain

Registry Number: EC 3.4.22.-

Grant and Affiliation Information for Requirement of different subdomains of calpastatin for calpain inhibition and for binding to calmodulin-like domains.

AFFILIATION: Institute for Virus Research, Kyoto University.

Country: JAPAN

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MEDLINETA: J Biochem

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