Reproducible isolation of distinct, overlapping segments of the phosphoproteome.

Reproducible isolation of distinct, overlapping segments of the phosphoproteome. Research Abstract Details 

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Reproducible isolation of distinct, overlapping segments of the phosphoproteome. Abstract Text:

Bernd Bodenmiller,Lukas N Mueller,Markus Mueller,Bruno Domon,Ruedi Aebersold,

The ability to routinely analyze and quantitatively measure changes in protein phosphorylation on a proteome-wide scale is essential for biological and clinical research. We assessed the ability of three common phosphopeptide isolation methods (phosphoramidate chemistry (PAC), immobilized metal affinity chromatography (IMAC) and titanium dioxide) to reproducibly, specifically and comprehensively isolate phosphopeptides from complex mixtures. Phosphopeptides were isolated from aliquots of a tryptic digest of the cytosolic fraction of Drosophila melanogaster Kc167 cells and analyzed by liquid chromatography-electrospray ionization tandem mass spectrometry. Each method reproducibly isolated phosphopeptides. The methods, however, differed in their specificity of isolation and, notably, in the set of phosphopeptides isolated. The results suggest that the three methods detect different, partially overlapping segments of the phosphoproteome and that, at present, no single method is sufficient for a comprehensive phosphoproteome analysis.

Reproducible isolation of distinct, overlapping segments of the phosphoproteome. Publishing Authors By Initials

B Bodenmiller,LN Mueller,M Mueller,B Domon,R Aebersold,

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Reproducible isolation of distinct, overlapping segments of the phosphoproteome. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nature methods

VOLUME: 4

Page Numbers: 231-7

Journal Abbreviation: Nat. Methods

ISSN: 1548-7091

DAY: 11

MONTH: 02

YEAR: 2007

Reproducible isolation of distinct, overlapping segments of the phosphoproteome. Information

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LANGUAGE: eng

NlmUniqueID: 101215604

Reproducible isolation of distinct, overlapping segments of the phosphoproteome. Keywords Mesh Terms:

KEYWORDS: Spectrometry, Mass, Electrospray Ionizat

MESH TERMS: methods

Chemical & Substance for Abstract: Reproducible isolation of distinct, overlapping segments of the phosphoproteome. Information

Substance Name: Proteome

Registry Number: 0

Grant and Affiliation Information for Reproducible isolation of distinct, overlapping segments of the phosphoproteome.

AFFILIATION: Institute for Molecular Systems Biology, Swiss Federal Institute of Technology, ETH Hönggerberg, Wolfgang-Pauli-Str. 16, HPT, CH-8093 Zürich, Switzerland.

Country: United States

AGENCY: United States NHLBI

GRANT: N01-HV-28179

ACRONYM: HV

MEDLINETA: Nat Methods

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