Repression of BK virus infection of human renal proximal tubular epithelial cells by pravastatin.

Repression of BK virus infection of human renal proximal tubular epithelial cells by pravastatin. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Repression of BK virus infection of human renal proximal tubular epithelial cells by pravastatin. Abstract Text:

BACKGROUND: BK virus (BKV), a human polyomavirus, causes BKV nephritis, which often leads to graft loss after renal transplantation. Currently, the only efficient therapy against BKV nephritis seems to be a reduction or change of immunosuppressive agents, but this may increase the inherent risk of rejection. Here, we report the ability of 3-hydroxy-3-methyl-glutaryl coenzyme A reductase inhibitor (statin), which is routinely used to treat hypercholesterolemia, to repress BKV entry pathways in human renal proximal tubular epithelial cells (HRPTEC) and, correspondently, prevent BKV infection. METHODS: HRPTEC were co-incubated with BKV and pravastatin. Then the percentage of HRPTEC infected with BKV by immunofluorescent analysis and large T-antigen expression which suggested BKV infection by Western blots was assessed in the absence and presence of pravastatin. The distribution of purified and labeled BKV particles in the presence and absence of pravastatin was also investigated. RESULTS: Both the percentage of BKV infected cells and the large T-antigen expression were significantly decreased in HRPTEC pretreated and co-incubated with pravastatin. However, when pravastatin was added 72 hr after BKV infection it failed to decrease percentage of BKV infected cells. It is likely, that pravastatin's inhibitory effect is explained by depletion of caveolin-1, a critical element of caveolae. BKV enters HRPTEC by caveolar-mediated endocytosis. We provide evidence that pravastatin dramatically decreased caveolin-1 expression in HRPTEC and interfered with internalization of labeled BKV particles. CONCLUSIONS: Our data suggest that pravastatin, acting through depletion of caveolin-1, prevented caveolar-dependent BKV internalization and repressed BKV infection of HRPTEC.

Repression of BK virus infection of human renal proximal tubular epithelial cells by pravastatin. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Repression of BK virus infection of human renal proximal tubular epithelial cells by pravastatin. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Transplantation

VOLUME: 85

Page Numbers: 1311-7

Journal Abbreviation: Transplantation

ISSN: 0041-1337

DAY: 15

MONTH: May

YEAR: 2008

Repression of BK virus infection of human renal proximal tubular epithelial cells by pravastatin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 132144

Repression of BK virus infection of human renal proximal tubular epithelial cells by pravastatin. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Repression of BK virus infection of human renal proximal tubular epithelial cells by pravastatin. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Repression of BK virus infection of human renal proximal tubular epithelial cells by pravastatin.

AFFILIATION: Division of Nephrology and Kidney Disease Center, Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: R01 HL022563

ACRONYM: HL

MEDLINETA: Transplantation

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Repression of BK virus infection of human renal proximal tubular epithelial cells by pravastatin Related Publications

• Caveolar endocytosis is critical for BK virus infection of human renal proximal tubular epithelial cells.
• Cranial nerve palsy in tetanus.
• Endothelin 1 induces beta 1Pix translocation and Cdc42 activation via protein kinase A-dependent pathway.
• Endothelin-1 Couples {beta}Pix to p66Shc: Role of {beta}Pix in Cell Proliferation through FOXO3a Phosphorylation and p27kip1 Down-Regulation Independently of Akt.
• Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity.
• Haloalkaliphilic diazotrophs in soda solonchak soils.
• Heterotrophic denitrification at extremely high salt and pH by haloalkaliphilic Gammaproteobacteria from hypersaline soda lakes.
• Intracellular trafficking pathway of BK Virus in human renal proximal tubular epithelial cells.
• Kinetics of luminescence and of electron transport in photosystem. II. When electron transport on acceptor side of this system is blocked.
• Methods to investigate the possibilities of using a three element periodic structure to suppress the transmission of energy in an elastic tube.
• Protein kinase A-dependent phosphorylation modulates beta1Pix guanine nucleotide exchange factor activity through 14-3-3beta binding.
• Repression of BK virus infection of human renal proximal tubular epithelial cells by pravastatin.
• The extracellular matrix of the spleen as a potential organizer of immune cell compartments.
• The genetically remote pathogenic strain NVH391-98 of the Bacillus cereus group is representative of a cluster of thermophilic strains.
• [Chemistry of hydrogen reduction of sulfates.]
• [Effect of acute stress on structuro-functional parameters of mouse liver and adrenal cortex]
• [Evaluation of the immune status in cattle herds with foot-and-mouth disease]
• [For the prevention of foot-and-mouth disease]
• [Investigation on certain aspects of Bacillus acetoethylicus using ferment poisons.]
• [Method of determination of the rate of running by fragments of the distance.]
• [Multiply achievements, eliminate deficiencies]
• [New methods of isolation of sulfate-reducing bacteria.]
• [On bacterial chemosynthesis in the Black Sea]
• [Prevention of noninfectious diseases among animals in Kaluga Province]
• [Primary transitional cell carcinoma of the prostate gland]
• [Skeletomuscular cell and its activity in the entire organism in relation to the effect of the nervous system.]
• [The antitoxic and pyrogenic properties of rabbit polymorphonuclear leukocytes]
• [The comparative effects of actinomycin D, imuren and hydrocortisone on interferon and antibody formation in mice]
• [The therapy of calves with toxic dyspepsia]
• [The treatment of dyspepsia in calves with titrated antibiotics and nitrofurans]