[Reporter genes transfer into human squamous cell carcinoma of larynx factor receptor mediated non-viral GE7 polypeptide delivery system with a new epidermal growth]

[Reporter genes transfer into human squamous cell carcinoma of larynx factor receptor mediated non-viral GE7 polypeptide delivery system with a new epidermal growth] Research Abstract Details 

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[Reporter genes transfer into human squamous cell carcinoma of larynx factor receptor mediated non-viral GE7 polypeptide delivery system with a new epidermal growth] Abstract Text:

Shui-fang Xiao,Lin Xi,Yi-ding Zhou,Cheng-yuan Wang,Pei-kun Tian,Jian-ren Gu,

OBJECTIVE: To evaluate the efficiency of marker gene and reporter gene transfection into human squamous cell carcinoma of larynx implanted in nude mice with a new EGFR mediated non-viral polypeptide gene delivery system. METHODS: Labeled streptavidin biotin (LSAB) was applied to examine over-expression of epidermal growth factor receptor (EGFR) on the squamous cell carcinoma of larynx. The complex of marker gene or reporter gene and gene delivery system was prepared and subsequently inoculated with Hep 2 cells or injected into laryngeal carcinoma imptanted on nude mice. RESULTS: Over-expression of EGFR was observed on inoculated Hep 2 cells and on 65% of laryngeal carcinoma specimen examined (15/23). Positive staining was located on the surface of the cells and some of them in the cytoplasma. Green fluorescence was observed on Hep 2 cells under fluorescent microscope 48 hrs after PEGFP-C1 gene transfection and became the strongest 72 hrs after transfection. The rate of transfection was 80% (78/97). Blue staining was noticed 12 hrs after beta-gal gene transfer and it became more remarkable 24 hrs after gene transfection with X-gal staining on the implanted human laryngeal carcinoma on nude mice. CONCLUSIONS: Squamous cell carcinoma of head and neck over-expressed EGFR and a non-viral GE7 polypeptide gene delivery system could transfer exogenous genes into laryngeal carcinoma cells and tissues with high efficiency and targetability both in vitro and in vivo suggesting that GE7 would be applicable as a new targeted, high efficient gene delivery system to the gene therapy of squamous cell carcinoma of head and neck.

[Reporter genes transfer into human squamous cell carcinoma of larynx factor receptor mediated non-viral GE7 polypeptide delivery system with a new epidermal growth] Publishing Authors By Initials

SF Xiao,L Xi,YD Zhou,CY Wang,PK Tian,JR Gu,

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[Reporter genes transfer into human squamous cell carcinoma of larynx factor receptor mediated non-viral GE7 polypeptide delivery system with a new epidermal growth] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhonghua er bi yan hou ke za zhi

VOLUME: 39

Page Numbers: 283-7

Journal Abbreviation: Zhonghua Er Bi Yan Hou Ke Za Z

ISSN: 0412-3948

DAY: 1

MONTH: May

YEAR: 2004

[Reporter genes transfer into human squamous cell carcinoma of larynx factor receptor mediated non-viral GE7 polypeptide delivery system with a new epidermal growth] Information

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LANGUAGE: chi

NlmUniqueID: 16210350

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AFFILIATION: Department of Otorhinolaryngology-Head and Neck Surgery, First Hospital of Peiking University, Beijing 100034, China. xsfxdd@public.bta.net.cn

Country: China

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MEDLINETA: Zhonghua Er Bi Yan Hou Ke Za Z

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