Replication of a DNA microarray.

Replication of a DNA microarray. Research Abstract Details 

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Replication of a DNA microarray. Abstract Text:

Haohao Lin,Li Sun,Richard M Crooks,

A mechanical method for efficient replication of DNA microarrays is described. The approach consists of three steps. First, a master DNA microarray consisting of single-stranded DNA elements is exposed to a solution containing the biotin-functionalized complement of each array element. Following hybridization, a replica surface modified with streptavidin is brought into contact with the master. This results in linking of the biotin-functionalized complement with the replica surface. Next, the replica is separated from the master, and the complementary strands are transferred to the replica surface. The resulting complementary DNA microarray contains position-coded sequences that mirror the information contained on the master DNA microarray. Multiple replicas can be prepared from a single master, the replicas efficiently hybridize only their complement, and DNA not labeled with biotin is not transferred to the replica surface.

Replication of a DNA microarray. Publishing Authors By Initials

H Lin,L Sun,RM Crooks,

For similar investigative techniques: chemistry, analytical: microchip analytical procedures: microarray analysis: oligonucleotide array sequence analysis research abstracts see: investigative techniques: chemistry, analytical: microchip analytical procedures: microarray analysis: oligonucleotide array sequence analysis research

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Replication of a DNA microarray. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 127

Page Numbers: 11210-1

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 17

MONTH: Aug

YEAR: 2005

Replication of a DNA microarray. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

Replication of a DNA microarray. Keywords Mesh Terms:

KEYWORDS: Oligonucleotide Array Sequence Analysis

MESH TERMS: methods

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Grant and Affiliation Information for Replication of a DNA microarray.

AFFILIATION: Department of Chemistry, Texas A&M University, P.O. Box 30012, College Station, Texas 77842-3012, USA.

Country: United States

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MEDLINETA: J Am Chem Soc

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