Renal cells activate the platelet receptor CLEC-2 through podoplanin.

Renal cells activate the platelet receptor CLEC-2 through podoplanin. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Renal cells activate the platelet receptor CLEC-2 through podoplanin. Abstract Text:

Charita M Christou,Andrew C Pearce,Aleksandra A Watson,Anita R Mistry,Alice Y Pollitt,Angharad E Fenton-May,Louise A Johnson,David G Jackson,Steve P Watson,Chris A O'Callaghan,

We have recently shown that the C-type lectin-like receptor, CLEC-2, is expressed on platelets and that it mediates powerful platelet aggregation by the snake venom toxin rhodocytin. In addition, we have provided indirect evidence for an endogenous ligand for CLEC-2 in renal cells expressing HIV-1. This putative ligand facilitates transmission of HIV through its incorporation into the viral envelope and binding to CLEC-2 on platelets. The aim of the present study was to identify the ligand on these cells which binds to CLEC-2 on platelets. Recombinant CLEC-2 exhibits specific binding to HEK-293T (human embryonic kidney) cells in which the HIV can be grown. Furthermore, HEK-293T cells activate both platelets and CLEC-2-transfected DT-40 B-cells. The transmembrane protein podoplanin was identified on HEK-293T cells and was demonstrated to mediate both binding of HEK-293T cells to CLEC-2 and HEK-293T cell activation of CLEC-2-transfected DT-40 B-cells. Podoplanin is expressed on renal cells (podocytes). Furthermore, a direct interaction between CLEC-2 and podoplanin was confirmed using surface plasmon resonance and was shown to be independent of glycosylation of CLEC-2. The interaction has an affinity of 24.5+/-3.7 muM. The present study identifies podoplanin as a ligand for CLEC-2 on renal cells.

Renal cells activate the platelet receptor CLEC-2 through podoplanin. Publishing Authors By Initials

CM Christou,AC Pearce,AA Watson,AR Mistry,AY Pollitt,AE Fenton-May,LA Johnson,DG Jackson,SP Watson,CA O'Callaghan,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Renal cells activate the platelet receptor CLEC-2 through podoplanin. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Biochemical journal

VOLUME: 411

Page Numbers: 133-40

Journal Abbreviation:

ISSN: 1470-8728

DAY: 1

MONTH: Apr

YEAR: 2008

Renal cells activate the platelet receptor CLEC-2 through podoplanin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2984726

Renal cells activate the platelet receptor CLEC-2 through podoplanin. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Renal cells activate the platelet receptor CLEC-2 through podoplanin. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Renal cells activate the platelet receptor CLEC-2 through podoplanin.

AFFILIATION: Henry Wellcome Building for Molecular Physiology, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, U.K.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Biochem J

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Renal cells activate the platelet receptor CLEC-2 through podoplanin Related Publications

• A comparative study of self-perceived public health competencies: practice teachers and qualifying SCPHNs.
• An assessment of the potential of herbivorous insect gut bacteria to develop competence for natural transformation.
• Autoantibodies to neurotypic and gliotypic proteins as biomarkers of neurotoxicity: Assessment of trimethyltin (TMT).
• Biochemical studies of the maturation of herpesvirus nucleocapsid species.
• Corneal virulence of Pseudomonas aeruginosa elastase B and alkaline protease produced by Pseudomonas putida.
• Crystal structure of rhodocytin, a ligand for the platelet-activating receptor CLEC-2.
• Delivery systems: the science.
• Divergent roles for tumor necrosis factor-alpha in the brain.
• Do early-life insults contribute to the late-life development of Parkinson and Alzheimer diseases?
• Do neonates have a nasal cycle?
• Do relatives of elderly patients block the discharge process?
• Dr. Edmund Bailey O'Callaghan, his early years in medicine, Montreal, 1823-1828.
• Effect of cutting time, temperature, and calcium on curd moisture, whey fat losses, and curd yield by response surface methodology.
• Effect of rumen degradable bolus containing melatonin or progestagen pessary plus pregnant mare serum gonadotropin on estrus response and lambing rate in ewes.
• Effectiveness of a new tobramycin (0.3%) and dexamethasone (0.05%) formulation in the treatment of experimental Pseudomonas keratitis.
• Evaluating mid-infrared spectroscopy as a new technique for predicting sensory texture attributes of processed cheese.
• Fluoroquinolone therapy in a rabbit model of post-LASIK methicillin-resistant Staphylococcus aureus keratitis.
• Innate pulmonary immunity: cilia.
• Is it legal to offer discounts for cash?
• Low-level human equivalent gestational lead exposure produces supernormal scotopic electroretinograms, increased retinal neurogenesis, and decreased retinal dopamine utilization in rats.
• Lullament: lullaby and lament therapeutic qualities actualized through music therapy.
• Novel online sensor technology for continuous monitoring of milk coagulation and whey separation in cheesemaking.
• Predictors of outcome in first-episode schizophrenia over the first 4 years of illness.
• Prevalence and correlates of mixed-handedness in schizophrenia.
• Renal cells activate the platelet receptor CLEC-2 through podoplanin.
• Schizophrenia and neurological soft signs: gender differences in clinical correlates and antecedent factors.
• Streptococcus pneumoniae Deficient in Pneumolysin or Autolysin Has Reduced Virulence in Meningitis.
• Surfactant protein D in Pseudomonas aeruginosa keratitis.
• The delivery of proteins and peptides to the lung.
• Tuberous sclerosis--what's new?