Removal of troponin C and desensitization of myosin B from ascidian smooth muscle by treatment with ethylene diamine tetraacetate.

Removal of troponin C and desensitization of myosin B from ascidian smooth muscle by treatment with ethylene diamine tetraacetate. Research Abstract Details 

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Removal of troponin C and desensitization of myosin B from ascidian smooth muscle by treatment with ethylene diamine tetraacetate. Abstract Text:

I Miyakawa,K Konishi,

On treatment with 10 mM EDTA at 30 degrees C, protein of 18,000 daltons was released from myofibrils, thin filaments and myosin B prepared from the smooth muscle of an ascidian, Halocynthia roretzi. This protein was purified from the EDTA extract of myofibrils by differential centrifugation, freeze-drying and gel-filtration. Based on its molecular weight, electrophoretic mobilities in the presence and absence of Ca2+ and other properties, it was identified as troponin C. By EDTA treatment, ascidian myosin B lost the Ca2+-sensitivity of Mg2+-ATPase, and EDTA-treated myosin B recovered the sensitivity by mixing with the EDTA extract of myosin B in the presence of Mg2+. Gel-electrophoretic patterns indicated that desensitization and resensitization of ascidian myosin B were accompanied by the removal and binding of troponin C. These results indicate that ascidian smooth muscle is regulated by a troponin-tropomyosin system, and desensitization induced by EDTA treatment is due to the removal of troponin C but not the release of the light chains of the myosin molecule. Based on these findings, we have established a simple method for the purification of troponin C from ascidian smooth muscle.

Removal of troponin C and desensitization of myosin B from ascidian smooth muscle by treatment with ethylene diamine tetraacetate. Publishing Authors By Initials

I Miyakawa,K Konishi,

For similar animals: chordata: chordata, nonvertebrate: urochordata research abstracts see: animals: chordata: chordata, nonvertebrate: urochordata research

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Removal of troponin C and desensitization of myosin B from ascidian smooth muscle by treatment with ethylene diamine tetraacetate. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 95

Page Numbers: 57-65

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1984

Removal of troponin C and desensitization of myosin B from ascidian smooth muscle by treatment with ethylene diamine tetraacetate. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Removal of troponin C and desensitization of myosin B from ascidian smooth muscle by treatment with ethylene diamine tetraacetate. Keywords Mesh Terms:

KEYWORDS: Urochordata

MESH TERMS: analysis

Chemical & Substance for Abstract: Removal of troponin C and desensitization of myosin B from ascidian smooth muscle by treatment with ethylene diamine tetraacetate. Information

Substance Name: Myosins

Registry Number: EC 3.6.1.4

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Country: JAPAN

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MEDLINETA: J Biochem

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