Removal of swine zonae pellucidae with sequential exposures to pronase and acidified medium.

Removal of swine zonae pellucidae with sequential exposures to pronase and acidified medium. Research Abstract Details 

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Removal of swine zonae pellucidae with sequential exposures to pronase and acidified medium. Abstract Text:

W M Graves,D Olds,L A Edgerton,

Exposure to acidified PBS (pH 3) for 60 sec removed swine zonae pellucidae from 70.4% of 27 swine morulae, and 73.7% of these formed blastocysts in culture. Further investigations revealed that treating embryos with 0.5% pronase and acidified PBS (pH 3) for 30 sec each was more effective. Zonae were removed in 90.6% of 85 embryos (four-cell to morulae) treated. A total of 76.9% of 65 zona-free embryos and 81.6% of 38 untreated embryos formed blastocysts (P > 0.05). An additional 57 untreated and 49 zona-free embryos (morulae to blastocysts) were transferred to seven recipient sows. Four sows returned to estrus (18 to 27 days), but three others were pregnant when slaughtered at 38 to 42 days. One pregnant sow had received a combination of five zona-free and six untreated embryos and demonstrated the potential for further development of treated embryos in vivo.

Removal of swine zonae pellucidae with sequential exposures to pronase and acidified medium. Publishing Authors By Initials

WM Graves,D Olds,LA Edgerton,

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Removal of swine zonae pellucidae with sequential exposures to pronase and acidified medium. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 22

Page Numbers: 135-42

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Aug

YEAR: 1984

Removal of swine zonae pellucidae with sequential exposures to pronase and acidified medium. Information

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LANGUAGE: eng

NlmUniqueID: 421510

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Grant and Affiliation Information for Removal of swine zonae pellucidae with sequential exposures to pronase and acidified medium.

AFFILIATION: Department of Animal Sciences University of Kentucky Lexington, KY 40546-0215 USA.

Country: United States

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MEDLINETA: Theriogenology

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