Removal of proteases from Clostridium perfringens fermented broth by aqueous two-phase systems (PEG/citrate).

Removal of proteases from Clostridium perfringens fermented broth by aqueous two-phase systems (PEG/citrate). Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Removal of proteases from Clostridium perfringens fermented broth by aqueous two-phase systems (PEG/citrate). Abstract Text:

Tatiana Souza Porto, , Neto, Filho,Attilio Converti, Porto,Adalberto Pessoa,

In order to reduce the toxicity of Clostridium perfringens fermentation broths used in vaccine preparation, we developed two-phase aqueous systems for removal of toxin-activating proteases. Removal of the proteases inhibits the conversion of protoxin to active toxin. In order to establish the conditions under which the phase separation occurs, binodal curves, formed by poly(ethylene glycol) (PEG) and sodium citrate, were investigated at different values of pH and PEG molar mass. A 2(4)-experimental design was used to evaluate the influence of PEG molar mass and concentration, citrate concentration and pH on protease partition coefficient, removal.factor and protease removal yield. It has been found that simultaneous increase in PEG molar mass and decrease in citrate concentration remarkably improved the removal factor, whereas the protease removal yield showed an opposite trend. The best conditions for the system under consideration (removal factor of 2.69 and yield of 116%) were obtained at pH 8.0 using PEG molar mass of 8000 g mol(-1) and concentrations of PEG and citrate of 24 and 15%, respectively.

Removal of proteases from Clostridium perfringens fermented broth by aqueous two-phase systems (PEG/citrate). Publishing Authors By Initials

TS Porto,PA ,BB Neto,JL Filho,A Converti,AL Porto,A Pessoa,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Removal of proteases from Clostridium perfringens fermented broth by aqueous two-phase systems (PEG/citrate). Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of industrial microbiology & biotechnology

VOLUME: 34

Page Numbers: 547-52

Journal Abbreviation: J. Ind. Microbiol. Biotechnol.

ISSN: 1367-5435

DAY: 24

MONTH: Aug

YEAR: 2007

Removal of proteases from Clostridium perfringens fermented broth by aqueous two-phase systems (PEG/citrate). Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9705544

Removal of proteases from Clostridium perfringens fermented broth by aqueous two-phase systems (PEG/citrate). Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Removal of proteases from Clostridium perfringens fermented broth by aqueous two-phase systems (PEG/citrate). Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Removal of proteases from Clostridium perfringens fermented broth by aqueous two-phase systems (PEG/citrate).

AFFILIATION: Department of Biochemical and Pharmaceutical Technology, University of São Paulo, 05508-000 Sio Paulo, SP, Brazil. portots@usp.br

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Ind Microbiol Biotechnol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Removal of proteases from Clostridium perfringens fermented broth by aqueous two-phase systems PEG/citrate Related Publications

• Adverse reactions of fluorescein angiography: a prospective study.
• Affective learning enhances visual detection and responses in primary visual cortex.
• Affective learning increases sensitivity to graded emotional faces.
• Affective learning modulates spatial competition during low-load attentional conditions.
• Antiproliferative activity of pristimerin isolated from Maytenus ilicifolia (Celastraceae) in human HL-60 cells.
• Behavioural evidence of sex-linked colour vision polymorphism in the squirrel monkey Saimiri ustus.
• Color vision in the black howler monkey ( Alouatta caraya).
• Cytotoxicity and genotoxicity of ingenamine G isolated from the Brazilian marine sponge Pachychalina alcaloidifera.
• Evaluation of mutagenic effects of formocresol: detection of DNA-protein cross-links and micronucleus in mouse bone marrow.
• Fear perception: can objective and subjective awareness measures be dissociated?
• NMR spectral assignments of a new [C--O--C] isoflavone dimer from Andira surinamensis.
• Nematicidal and larvicidal activities of the essential oils from aerial parts of Pectis oligocephala and Pectis apodocephala Baker.
• Not Available
• On the relationship between emotion and cognition.
• Perceptual filling-in of stereoscopically presented targets.
• Perceptual visual filling-in of toxoplasmic retinochoroiditis scotomas.
• Phase diagrams of a CTAB/organic solvent/buffer system applied to extraction of enzymes by reverse micelles.
• Protein partitioning in poly(ethylene glycol)/sodium polyacrylate aqueous two-phase systems.
• Removal of proteases from Clostridium perfringens fermented broth by aqueous two-phase systems (PEG/citrate).
• Sustained and transient modulation of performance induced by emotional picture viewing.
• [Clinical experience with a new antispasmodic-analgesic combination (preliminary note)]
• [Clinico-therapeutic trial of a new amebicide.]
• [Emotional stimuli: sensory processing and motor responses]
• [Low prevalence of dyschromatopsia using the fourth edition of HRR (Hardy, Rand and Rittler) pseudoisochromatic plate test among the Indian population of Lalima village, Terena]
• [Not Available.]
• [Not Available.]
• [Samuel Barnsley Pessôa: bio-bibliographical data.]
• [Schistosomiasis mansoni. Epidemiology.]
• [THE USE OF AN ANORETIC SUBSTANCE IN OBESE DIABETICS.]
• [Trypanosoma hogei n. sp., parasite of the false cobra(Rachidelus brazili Boul.)]