Released ATP is an extracellular cytotoxic mediator in salivary histatin 5-induced killing of Candida albicans.

Released ATP is an extracellular cytotoxic mediator in salivary histatin 5-induced killing of Candida albicans. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Released ATP is an extracellular cytotoxic mediator in salivary histatin 5-induced killing of Candida albicans. Abstract Text:

Salivary histatins (Hsts) are antifungal peptides with promise as therapeutic agents against candidiasis. Hst 5 kills the fungal pathogen Candida albicans via a mechanism that involves release of cellular ATP in the absence of cytolysis. Here we demonstrate that released ATP has a further role in Hst 5 killing. Incubation of the cells with ATP analogues induced cell death, and addition of the ATP scavenger apyrase to remove extracellular ATP released during Hst 5 treatment resulted in a reduction in cell killing. Experiments using anaerobically grown C. albicans with decreased susceptibility to Hst 5 confirmed that depletion of cellular ATP as a result of ATP efflux was not sufficient to cause cell death. In contrast to Hst-susceptible aerobic cultures, anaerobically grown cells were not killed by exogenously applied ATP. These findings established that Hst binding, subsequent entry into the cells, and ATP release precede the signal for cytotoxicity, which is mediated by extracellular ATP. In a higher-eukaryote paradigm, released ATP acts as a cytotoxic mediator by binding to membrane nucleotide P2X receptors. Based on a pharmacological profile and detection of a C. albicans 60-kDa membrane protein immunoreactive with antibody to P2X(7) receptor, we propose that released ATP in response to Hst 5 activates candidal P2X(7)-like receptors to cause cell death.

Released ATP is an extracellular cytotoxic mediator in salivary histatin 5-induced killing of Candida albicans. Publishing Authors By Initials

For similar proteins: salivary proteins research abstracts see: proteins: salivary proteins research

PUBMED ID PMID:

MEDLINE DATE:

Released ATP is an extracellular cytotoxic mediator in salivary histatin 5-induced killing of Candida albicans. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Infection and immunity

VOLUME: 68

Page Numbers: 6848-56

Journal Abbreviation: Infect. Immun.

ISSN: 0019-9567

DAY: 18

MONTH: Dec

YEAR: 2000

Released ATP is an extracellular cytotoxic mediator in salivary histatin 5-induced killing of Candida albicans. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 246127

Released ATP is an extracellular cytotoxic mediator in salivary histatin 5-induced killing of Candida albicans. Keywords Mesh Terms:

KEYWORDS: Salivary Proteins

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Released ATP is an extracellular cytotoxic mediator in salivary histatin 5-induced killing of Candida albicans. Information

Substance Name: Adenosine Triphosphate

Registry Number: 56-65-5

Grant and Affiliation Information for Released ATP is an extracellular cytotoxic mediator in salivary histatin 5-induced killing of Candida albicans.

AFFILIATION: Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, Buffalo, New York 14214, USA.

Country: UNITED STATES

AGENCY: United States NIDCR

GRANT: R01 DE010641-08

ACRONYM: DE

MEDLINETA: Infect Immun

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Released ATP is an extracellular cytotoxic mediator in salivary histatin 5-induced killing of Candida albicans Related Publications

• A multiple-electrode cochlear implant.
• Abnormal vaginal bleeding.
• Acute effects of exercise on MR imaging of skeletal muscle: concentric vs eccentric actions.
• Customers are more than consumers. Realize the value of marketing to EMTs.
• Differential modulation of myosin heavy chain phenotype in an inactive extensor and flexor muscle of adult rats.
• Effect of clomiphene citrate on pituitary responsiveness to gonadotropin releasing hormone in rams and wethers.
• Effects of season and lactation on luteinizing hormone secretion in postpartum ewes.
• Electromechanical stimulation ameliorates inactivity-induced adaptations in the medial gastrocnemius of adult rats.
• Epidural spinal cord stimulation plus quipazine administration enable stepping in complete spinal adult rats.
• Epidural stimulation: Comparison of the spinal circuits that generate and control locomotion in rats, cats and humans.
• Flexor bias of joint position in humans during spaceflight.
• Focus groups can help drive patient satisfaction.
• Genetically engineered human salivary histatin genes are functional in Candida albicans: development of a new system for studying histatin candidacidal activity.
• Histatin 5 initiates osmotic stress response in Candida albicans via activation of the Hog1 mitogen-activated protein kinase pathway.
• Insulin action on the liver in vivo.
• Irradiation as a causative factor in thyroid cancer.
• Is spinal cord isolation a good model of muscle disuse?
• Plasticity of functional connectivity in the adult spinal cord.
• Proceedings: Rehabilitation of the oral cavity by plastic surgery after cancer resections.
• Psychoelectric measurements and results from cochlear implant patients.
• Public Health Applications of High-Speed Photography.
• Rat alpha- and gamma-motoneuron soma size and succinate dehydrogenase activity are independent of neuromuscular activity level.
• Reconstruction of the hypopharynx and the cervical esophagus after removal of cancer.
• Released ATP is an extracellular cytotoxic mediator in salivary histatin 5-induced killing of Candida albicans.
• Removal of swine zonae pellucidae with sequential exposures to pronase and acidified medium.
• Spinal cord reflexes induced by epidural spinal cord stimulation in normal awake rats.
• The P-113 Fragment of Histatin 5 Requires a Specific Peptide Sequence for Intracellular Translocation in Candida albicans, Which Is Independent of Cell Wall Binding.
• The Role of Muscle Activity in Health Maintenance for Spinal Cord Injury Patients: 298: 9:20 AM-9:45 AM.
• The role of low-dosage irradiation in selected cases of menstrual disorders.
• Tools for understanding and optimizing robotic gait training.