Relative strength of cation-pi vs salt-bridge interactions: the Gtalpha(340-350) peptide/rhodopsin system.

Relative strength of cation-pi vs salt-bridge interactions: the Gtalpha(340-350) peptide/rhodopsin system. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Relative strength of cation-pi vs salt-bridge interactions: the Gtalpha(340-350) peptide/rhodopsin system. Abstract Text:

Matthew A Anderson,Benhur Ogbay,Rieko Arimoto,Wei Sha,Oleg G Kisselev,David P Cistola,Garland R Marshall,

Interactions between cationic and aromatic side chains of amino acid residues, the so-called cation-pi interaction, are thought to contribute to the overall stability of the folded structure of peptides and proteins. The transferred NOE NMR structure of the G(t)alpha(340-350) peptide bound to photoactivated rhodopsin (R*) geometrically suggests a cation-pi interaction stabilizing the structure between the epsilon-amine of Lys341 and the aromatic ring of the C-terminal residue, Phe350. This interaction has been explored by varying substituents on the phenyl ring to alter the electron density of the aromatic ring of Phe350 and observing the impact on binding of the peptide to R*. The results suggest that while a cation-pi interaction geometrically exists in the G(t)alpha(340-350) peptide when bound to R*, its energetic contribution to the stability of the receptor-bound structure is relatively insignificant, as it was not observed experimentally. The presence of an adjacent and competing salt-bridge interaction between the epsilon-amine of Lys341 and the C-terminal carboxylate of Phe350 effectively shields the charge of the ammonium group. Experimental data supporting a significant cation-pi interaction can be regained through a series of Phe350 analogues where the C-terminal carboxyl has been converted to the neutral carboxamide, thus eliminating the shielding salt-bridge. TrNOE NMR experiments confirmed the existence of the cation-pi interaction in the carboxamide analogues. Various literature estimates of the strength of cation-pi interactions, including some that estimate strengths in excess of salt-bridges, are compromised by omission of the relevant anion in the calculations.

Relative strength of cation-pi vs salt-bridge interactions: the Gtalpha(340-350) peptide/rhodopsin system. Publishing Authors By Initials

MA Anderson,B Ogbay,R Arimoto,W Sha,OG Kisselev,DP Cistola,GR Marshall,

For similar inorganic chemicals: salts research abstracts see: inorganic chemicals: salts research

PUBMED ID PMID:

MEDLINE DATE:

Relative strength of cation-pi vs salt-bridge interactions: the Gtalpha(340-350) peptide/rhodopsin system. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 128

Page Numbers: 7531-41

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 14

MONTH: Jun

YEAR: 2006

Relative strength of cation-pi vs salt-bridge interactions: the Gtalpha(340-350) peptide/rhodopsin system. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

Relative strength of cation-pi vs salt-bridge interactions: the Gtalpha(340-350) peptide/rhodopsin system. Keywords Mesh Terms:

KEYWORDS: Salts

MESH TERMS: chemistry

Chemical & Substance for Abstract: Relative strength of cation-pi vs salt-bridge interactions: the Gtalpha(340-350) peptide/rhodopsin system. Information

Substance Name: Rhodopsin

Registry Number: 9009-81-8

Grant and Affiliation Information for Relative strength of cation-pi vs salt-bridge interactions: the Gtalpha(340-350) peptide/rhodopsin system.

AFFILIATION: Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110, USA.

Country: United States

AGENCY: United States NCRR

GRANT: P41RR00954

ACRONYM: RR

MEDLINETA: J Am Chem Soc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Relative strength of cation-pi vs salt-bridge interactions: the Gtalpha340-350 peptide/rhodopsin system Related Publications

• A matched case-control study: investigating the relationship between youth assets and sexual intercourse among 13- to 14-year-olds.
• A minimalistic 3D pharmacophore model for cyclopentapeptide CXCR4 antagonists.
• A multistranded polymer model explains MinDE dynamics in E. coli cell division.
• A new antibiotic combination for frozen bovine semen, 3. Evaluation of fertility.
• A new antibiotic combination for frozen bovine semen. 2. Evaluation of seminal quality.
• A new oral health elective for medical students at the University of Washington.
• A psychometric analysis of Project Liberty's adult enhanced services referral tool.
• Actinfilin is a Cul3 substrate adaptor, linking GluR6 kainate receptor subunits to the ubiquitin-proteasome pathway.
• Acute adverse effects of the indenopyridine CDB-4022 on the ultrastructure of sertoli cells, spermatocytes, and spermatids in rat testes: comparison to the known sertoli cell toxicant Di-n-pentylphthalate (DPP).
• Antibiotic management of suspected nosocomial ICU-acquired infection: does prolonged empiric therapy improve outcome?
• Conflict in the emergency department: Retreat in order to advance.
• Conformational templates for rational drug design: flexibility of cyclo(D-Pro1-Ala2-Ala3-Ala4-Ala5) in DMSO solution.
• Defining the interface between the C-terminal fragment of alpha-transducin and photoactivated rhodopsin.
• Do benzodiazepines mimic reverse-turn structures?
• Effect of music on patients undergoing outpatient colonoscopy.
• Engineering metal complexes of chiral pentaazacrowns as privileged reverse-turn scaffolds.
• Impact of a school-based dating violence prevention program among Latino teens: randomized controlled effectiveness trial.
• Interrelationships among fluorometric analyses of spermatozoal function, classical semen quality parameters and the fertility of frozen-thawed bovine spermatozoa.
• Modeling of the complex between transducin and photoactivated rhodopsin, a prototypical G-protein-coupled receptor.
• Novel trihydroxamate-containing peptides: design, synthesis, and metal coordination.
• Obesity and sex steroid changes across puberty: evidence for marked hyperandrogenemia in pre- and early pubertal obese girls.
• Peer and family influences on adolescent anger expression and the acceptance of cross-gender aggression.
• Progesterone acutely increases LH pulse amplitude but does not acutely influence nocturnal LH pulse frequency slowing during the late follicular phase in women.
• Protein recognition motifs: design of peptidomimetics of helix surfaces.
• Quantitation of human tissue and immune cell type II 14 kDa phospholipase A(2) by enzyme immunoassay.
• Relative strength of cation-pi vs salt-bridge interactions: the Gtalpha(340-350) peptide/rhodopsin system.
• Running wheel exercise enhances recovery from nigrostriatal dopamine injury without inducing neuroprotection.
• Temporal order and the evolution of complex acoustic signals.
• The regulation of FSHbeta transcription by gonadal steroids: testosterone and estradiol modulation of the activin intracellular signaling pathway.
• Violence and aggression in the emergency department: A critical care perspective.