Relative quantification of stable isotope labeled peptides using a linear ion trap-Orbitrap hybrid mass spectrometer.

Relative quantification of stable isotope labeled peptides using a linear ion trap-Orbitrap hybrid mass spectrometer. Research Abstract Details 

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Relative quantification of stable isotope labeled peptides using a linear ion trap-Orbitrap hybrid mass spectrometer. Abstract Text:

John D Venable,James Wohlschlegel,Daniel B McClatchy,Sung Kyu Park,John R Yates,

The quantitative analysis of complex biological samples has emerged as a key research area in the field of proteomics. Although quantitative proteomic experiments remain challenging, these strategies have been greatly facilitated by the development of newer high-performance mass spectrometers. In this work, we have evaluated the use of the LTQ-Orbitrap, a hybrid mass spectrometer in which a linear ion trap is coupled to an Orbitrap mass analyzer, for quantitative analyses. By analyzing a range of yeast protein standards, we found that the high mass accuracy, high resolution, large ion capacity, and large dynamic range of the LTQ-Orbitrap led to as much as a 4-5-fold improvement in the number and quality of the peptide ratio measurements compared to similar analyses done on the LTQ. We also successfully quantified protein expression differences that occur in metabolically labeled rat synapses during brain development to further demonstrate the suitability of the LTQ-Orbitrap for the comparative analysis of complex tissue samples.

Relative quantification of stable isotope labeled peptides using a linear ion trap-Orbitrap hybrid mass spectrometer. Publishing Authors By Initials

JD Venable,J Wohlschlegel,DB McClatchy,SK Park,JR Yates,

For similar cells: cellular structures: subcellular fractions: synaptosomes research abstracts see: cells: cellular structures: subcellular fractions: synaptosomes research

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Relative quantification of stable isotope labeled peptides using a linear ion trap-Orbitrap hybrid mass spectrometer. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Analytical chemistry

VOLUME: 79

Page Numbers: 3056-64

Journal Abbreviation:

ISSN: 0003-2700

DAY: 17

MONTH: 03

YEAR: 2007

Relative quantification of stable isotope labeled peptides using a linear ion trap-Orbitrap hybrid mass spectrometer. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370536

Relative quantification of stable isotope labeled peptides using a linear ion trap-Orbitrap hybrid mass spectrometer. Keywords Mesh Terms:

KEYWORDS: Synaptosomes

MESH TERMS: metabolism

Chemical & Substance for Abstract: Relative quantification of stable isotope labeled peptides using a linear ion trap-Orbitrap hybrid mass spectrometer. Information

Substance Name: Saccharomyces cerevisiae Proteins

Registry Number: 0

Grant and Affiliation Information for Relative quantification of stable isotope labeled peptides using a linear ion trap-Orbitrap hybrid mass spectrometer.

AFFILIATION: Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92014, USA.

Country: United States

AGENCY: United States NCRR

GRANT: P41RR11823-10

ACRONYM: RR

MEDLINETA: Anal Chem

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