Relationship between disruption of the unstirred mucus layer and intestinal restitution in loss of gut barrier function after trauma hemorrhagic shock.

Relationship between disruption of the unstirred mucus layer and intestinal restitution in loss of gut barrier function after trauma hemorrhagic shock. Research Abstract Details 

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Relationship between disruption of the unstirred mucus layer and intestinal restitution in loss of gut barrier function after trauma hemorrhagic shock. Abstract Text:

Bobby Rupani,Francis J Caputo,Anthony C Watkins,Dennis Vega,Louis J Magnotti,Qi Lu,Da Zhong Xu,Edwin A Deitch,

BACKGROUND: The factors involved in shock-induced loss of gut barrier function remain to be defined fully and studies investigating gut injury have focused primarily on the systemic side of the intestine. METHODS: Male Sprague-Dawley rats were subjected to a laparotomy (trauma) and 90 minutes of trauma sham shock (T/SS) or actual trauma (laparotomy) hemorrhagic shock (T/HS) (30 mm Hg). At 0, 30, 60, or 180 minutes after the end of shock and volume resuscitation (reperfusion), the animals were killed and samples of the ileum were collected for intestinal morphologic analysis, analysis of the unstirred mucus layer, and for barrier function by measuring permeability to flourescein dextran. RESULTS: T/HS-induced morphologic evidence of mucosal injury as well as epithelial apoptosis was present at the end of the shock period and maximal after 60 minutes of reperfusion. At 3 hours after reperfusion, the degree of villous injury and enterocyte apoptosis had decreased. In contrast to the morphologic appearance of the villi, disruption of the mucus layer became progressively more severe over time and was manifest as a decrease in mucus thickness, progressive loss of coverage of the luminal surface by the mucus layer, and a change in mucus appearance from a dense to a loose structure. Studies of intestinal permeability documented that T/HS-induced loss of gut barrier function persisted throughout the 3-hour reperfusion period and were associated with injury to the mucus layer as well as the villi. CONCLUSIONS: T/HS leads to changes in the intestinal mucus layer as well as increased villous injury, apoptosis, and gut permeability. Additionally, increased gut permeability was associated with loss of the intestinal mucus layer suggesting that T/HS-induced injury to the mucus layer may contribute to the loss of gut barrier function.

Relationship between disruption of the unstirred mucus layer and intestinal restitution in loss of gut barrier function after trauma hemorrhagic shock. Publishing Authors By Initials

B Rupani,FJ Caputo,AC Watkins,D Vega,LJ Magnotti,Q Lu,da Z Xu,EA Deitch,

For similar disorders of environmental origin: wounds and injuries: shock, traumatic research abstracts see: disorders of environmental origin: wounds and injuries: shock, traumatic research

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Relationship between disruption of the unstirred mucus layer and intestinal restitution in loss of gut barrier function after trauma hemorrhagic shock. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Surgery

VOLUME: 141

Page Numbers: 481-9

Journal Abbreviation: Surgery

ISSN: 0039-6060

DAY: 22

MONTH: 01

YEAR: 2007

Relationship between disruption of the unstirred mucus layer and intestinal restitution in loss of gut barrier function after trauma hemorrhagic shock. Information

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LANGUAGE: eng

NlmUniqueID: 417347

Relationship between disruption of the unstirred mucus layer and intestinal restitution in loss of gut barrier function after trauma hemorrhagic shock. Keywords Mesh Terms:

KEYWORDS: Shock, Traumatic

MESH TERMS: physiopathology

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Grant and Affiliation Information for Relationship between disruption of the unstirred mucus layer and intestinal restitution in loss of gut barrier function after trauma hemorrhagic shock.

AFFILIATION: Department of Surgery, University of Medicine and Dentistry of New Jersey-New Jersey Medical School, Newark, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: T32 GM 069330

ACRONYM: GM

MEDLINETA: Surgery

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