Relations between biochemical thermodynamics and biochemical kinetics.

Relations between biochemical thermodynamics and biochemical kinetics. Research Abstract Details 

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Relations between biochemical thermodynamics and biochemical kinetics. Abstract Text:

Robert A Alberty,

The parameters in steady-state or rapid-equilibrium rate equations for enzyme-catalyzed reactions depend on the temperature, pH, and ionic strength, and may depend on the concentrations of specific species in the buffer. When the complete rate equation (i.e. the equation with parameters for the reverse reaction as well as the forward reaction) is determined, there are one or more Haldane relations between some of the kinetic parameters and the apparent equilibrium constant for the reaction that is catalyzed. When the apparent equilibrium constant can be calculated from the kinetic parameters, the equilibrium composition can be calculated. This is remarkable because the kinetic parameters all depend on the properties of the enzymatic site, but the apparent equilibrium constant and the equilibrium composition do not. The effects of ionic strength and pH on the unoccupied enzymatic site and the occupied enzymatic site have to cancel in the Haldane relation or in the calculation of the apparent equilibrium constant using the rate constants for the steps in the mechanism. Several simple enzymatic mechanisms and their complete rate equations are discussed.

Relations between biochemical thermodynamics and biochemical kinetics. Publishing Authors By Initials

RA Alberty,

For similar natural sciences: physics: thermodynamics research abstracts see: natural sciences: physics: thermodynamics research

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Relations between biochemical thermodynamics and biochemical kinetics. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Biophysical chemistry

VOLUME: 124

Page Numbers: 11-7

Journal Abbreviation: Biophys. Chem.

ISSN: 0301-4622

DAY: 12

MONTH: 06

YEAR: 2006

Relations between biochemical thermodynamics and biochemical kinetics. Information

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LANGUAGE: eng

NlmUniqueID: 403171

Relations between biochemical thermodynamics and biochemical kinetics. Keywords Mesh Terms:

KEYWORDS: Thermodynamics

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Grant and Affiliation Information for Relations between biochemical thermodynamics and biochemical kinetics.

AFFILIATION: Department of Chemistry, Massachusetts Institute of Technology, Room 6-215, MIT, 77 Mass. Ave., Cambridge, MA 02139, USA. alberty@mit.edu

Country: Netherlands

AGENCY: United States NIGMS

GRANT: 5-R01-GM48348-10

ACRONYM: GM

MEDLINETA: Biophys Chem

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