Regulatory proteins of eukaryotic initiation factor 2-alpha subunit (eIF2alpha) phosphatase, under ischemic reperfusion and tolerance.

Regulatory proteins of eukaryotic initiation factor 2-alpha subunit (eIF2alpha) phosphatase, under ischemic reperfusion and tolerance. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Regulatory proteins of eukaryotic initiation factor 2-alpha subunit (eIF2alpha) phosphatase, under ischemic reperfusion and tolerance. Abstract Text:

Lidia ,Cristina Cid,Alberto ,Jozef Burda,Irene Ayuso,Matilde Salinas,Lidia ,Cristina Cid,Alberto ,Jozef Burda,Irene Ayuso,Matilde Salinas,

Phosphorylation of the alpha subunit of eukaryotic translation initiation factor 2 (eIF2alpha), which is one of the substrates of protein phosphatase 1 (PP1), occurs rapidly during the first minutes of post-ischemic reperfusion after an episode of cerebral ischemia. In the present work, two experimental models of transient global ischemia and ischemic tolerance (IT) were used to study PP1 interacting/regulatory proteins following ischemic reperfusion. For that purpose we utilized PP1 purified by microcystin chromatography, as well as 2D DIGE of PP1alpha and PP1gamma immunoprecipitates. The highest levels of phosphorylated eIF2alpha found after 30 min reperfusion in rats without IT, correlated with increased levels in PP1 immunoprecipitates of the inhibitor DARPP32 as well as GRP78 and HSC70 proteins. After 4 h reperfusion, the levels of these proteins in PP1c complexes had returned to control values, in parallel to a significant decrease in eIF2alpha phosphorylated levels. IT that promoted a decrease in eIF2alpha phosphorylated levels after 30 min reperfusion induced the association of GADD34 with PP1c, while prevented that of DARPP32, GRP78, and HSC70. Different levels of HSC70 and DARPP32 associated with PP1alpha and PP1gamma isoforms, whereas GRP78 was only detected in PP1gamma immunoprecipitates. Here we suggest that PP1, through different signaling complexes with their interacting proteins, may modulate the eIF2alpha phosphorylation/dephosphorylation during reperfusion after a transient global ischemia in the rat brain. Of particular interest is the potential role of GADD34/PP1c complexes after tolerance acquisition.

Regulatory proteins of eukaryotic initiation factor 2-alpha subunit (eIF2alpha) phosphatase, under ischemic reperfusion and tolerance. Publishing Authors By Initials

L ,C Cid,A ,J Burda,I Ayuso,M Salinas,L ,C Cid,A ,J Burda,I Ayuso,M Salinas,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Regulatory proteins of eukaryotic initiation factor 2-alpha subunit (eIF2alpha) phosphatase, under ischemic reperfusion and tolerance. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of neurochemistry

VOLUME: 103

Page Numbers: 1368-80

Journal Abbreviation: J. Neurochem.

ISSN: 1471-4159

DAY: 30

MONTH: 08

YEAR: 2007

Regulatory proteins of eukaryotic initiation factor 2-alpha subunit (eIF2alpha) phosphatase, under ischemic reperfusion and tolerance. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985190

Regulatory proteins of eukaryotic initiation factor 2-alpha subunit (eIF2alpha) phosphatase, under ischemic reperfusion and tolerance. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Regulatory proteins of eukaryotic initiation factor 2-alpha subunit (eIF2alpha) phosphatase, under ischemic reperfusion and tolerance. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Regulatory proteins of eukaryotic initiation factor 2-alpha subunit (eIF2alpha) phosphatase, under ischemic reperfusion and tolerance.

AFFILIATION: Hospital Ramón y Cajal, Servicio de Bioquímica-Investigación, Madrid, Spain.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Neurochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Regulatory proteins of eukaryotic initiation factor 2-alpha subunit eIF2alpha phosphatase, under ischemic reperfusion and tolerance Related Publications

• "Competitive" also plays a role.
• Arabidopsis CBF1 and CBF3 have a different function than CBF2 in cold acclimation and define different gene classes in the CBF regulon.
• Changes in renal function in valvular and coronary patients.
• Comparison of stir bar sorptive extraction and solid-phase microextraction to determine halophenols and haloanisoles by gas chromatography-ion trap tandem mass spectrometry.
• Context-dependent selection of visuomotor maps.
• Do systemic corticosteroids improve acute outcomes in infants with REV bronchiolitis?
• Epiphytic dinoflagellates associated with ciguatera in the northwestern coast of Cuba.
• Estimating national landfill methane emissions: an application of the 2006 Intergovernmental Panel on Climate Change Waste Model in Panama.
• From Neuronal Activity to the Actin Cytoskeleton: A Role for CaMKKs and betaPIX in Spine Morphogenesis.
• How behavioral constraints may determine optimal sensory representations.
• Nebivolol in the treatment of chronic heart failure.
• Need for medical workup in psychiatric assessment.
• Neurotrophic effects of GnRH on neurite outgrowth and neurofilament protein expression in cultured cerebral cortical neurons of rat embryos.
• Not Available
• Phylogenetic and functional analysis of Arabidopsis RCI2 genes.
• Platyhelminth mitochondrial and cytosolic redox homeostasis is controlled by a single thioredoxin glutathione reductase and dependent on selenium and glutathione.
• Protein extraction from Phoenix dactylifera L. leaves, a recalcitrant material, for two-dimensional electrophoresis.
• Regulatory proteins of eukaryotic initiation factor 2-alpha subunit (eIF2alpha) phosphatase, under ischemic reperfusion and tolerance.
• Semiautomatic determination of furanic aldehydes in food and pharmaceutical samples by a stopped-flow injection analysis method.
• Silastic ring vertical gastric bypass: evolution of an open surgical technique, and review of 1,588 cases.
• Tapping healthcare resource by older mexicans with diabetes: how migration to the United States facilitates access.
• Transformation ability of fungi isolated from cork and grape to produce 2,4,6-trichloroanisole from 2,4,6-trichlorophenol.
• Wnt regulates axon behavior through changes in microtubule growth directionality: a new role for adenomatous polyposis coli.
• [Comorbidity of specific and generalized social anxiety in Spanish adolescents]
• [Measles in Paraguay: experience in the Hospital for Infectious and Tropical Diseases of Asunción]
• [Not Available.]
• [Not Available.]
• [Rural sanitation and the infrastructural projects, auxilliary to health projects]
• [Treatment of massive hemorrhage caused by gastric & duodenal ulcer: round table. I.]
• [Treatment of massive hemorrhage caused by gastric or duodenal ulcer: round table. II.]