Regulation of vitamin D-responsive gene expression by fluorinated analogs of calcitriol in rat osteoblastic ROB-C26 cells.

Regulation of vitamin D-responsive gene expression by fluorinated analogs of calcitriol in rat osteoblastic ROB-C26 cells. Research Abstract Details 

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Regulation of vitamin D-responsive gene expression by fluorinated analogs of calcitriol in rat osteoblastic ROB-C26 cells. Abstract Text:

Y Miyamoto,T Shinki,Y Ohyama,T Kasama,H Iwasaki,

We compared the activation of vitamin D-responsive genes by 24,24-difluorocalcitriol [F2-1 alpha,25(OH)2D3] and 26,26,26,27,27,27-hexafluorocalcitriol [F6-1 alpha,25(OH)2D3] with that by calcitriol [1 alpha,25(OH)2D3] in rat osteoblastic ROB-C26 cells. F2-1 alpha,25(OH)2D3 and F6-1 alpha, 25(OH)2D3 were ten times more potent than 1 alpha,25(OH)2D3 in inducing the expression of 1 alpha, 25(OH)2D3-24-hydroxylase (24-OHase) mRNA 6 h after adding vitamin D compounds. The lower affinity of these two fluorinated analogs compared with that of 1 alpha,25(OH)2D3 for vitamin D binding protein in serum (serum DBP) seemed to be partly involved in their increased ability to activate the 24-OHase gene. A time course study revealed that the expression of the 24-OHase and osteopontin mRNAs in the cells incubated with 1 alpha, 25(OH)2D3 and F2-1 alpha,25(OH)2D3 attained maximal levels at 6 h for 24-OHase mRNA and 18 h for osteopontin mRNA, the both decreased thereafter. On the contrary, F6-1 alpha,25(OH)2D3 increased the expression of 24-OHase and osteopontin exponentially until 72 h. While F2-1 alpha,25(OH)2[1 beta-3H]D3 was catabolized quickly by ROB-C26 cells, F6-1 alpha,25(OH)2[1 beta-3H]D3 was slowly and quantitatively converted into putative 26,26,26,27,27,27-hexafluoro-23S-hydroxy[1 beta-3H]calcitriol (F6-1 alpha,23S,25(OH)3[1 beta-3H]D3). This may explain why the time-course profiles of the accumulation of mRNAs for 24-OHase and osteopontin differed in the cells exposed to the fluorinated analogs. In addition to the longer retention, unknown up-regulating mechanisms appeared to be involved in the exponential activation of the 24-OHase and osteopontin genes induced by F6-1 alpha,25(OH)2D3.

Regulation of vitamin D-responsive gene expression by fluorinated analogs of calcitriol in rat osteoblastic ROB-C26 cells. Publishing Authors By Initials

Y Miyamoto,T Shinki,Y Ohyama,T Kasama,H Iwasaki,

For similar proteins: carrier proteins: vitamin d-binding protein research abstracts see: proteins: carrier proteins: vitamin d-binding protein research

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Regulation of vitamin D-responsive gene expression by fluorinated analogs of calcitriol in rat osteoblastic ROB-C26 cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 118

Page Numbers: 1068-76

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1995

Regulation of vitamin D-responsive gene expression by fluorinated analogs of calcitriol in rat osteoblastic ROB-C26 cells. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Regulation of vitamin D-responsive gene expression by fluorinated analogs of calcitriol in rat osteoblastic ROB-C26 cells. Keywords Mesh Terms:

KEYWORDS: Vitamin D-Binding Protein

MESH TERMS: blood

Chemical & Substance for Abstract: Regulation of vitamin D-responsive gene expression by fluorinated analogs of calcitriol in rat osteoblastic ROB-C26 cells. Information

Substance Name: vitamin D 24-hydroxylase

Registry Number: EC 1.14.-

Grant and Affiliation Information for Regulation of vitamin D-responsive gene expression by fluorinated analogs of calcitriol in rat osteoblastic ROB-C26 cells.

AFFILIATION: Department of Biochemistry, Showa University, Tokyo.

Country: JAPAN

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MEDLINETA: J Biochem

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