Regulation of the yeast TSA1 peroxiredoxin by ZAP1 is an adaptive response to the oxidative stress of zinc deficiency.

Regulation of the yeast TSA1 peroxiredoxin by ZAP1 is an adaptive response to the oxidative stress of zinc deficiency. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Regulation of the yeast TSA1 peroxiredoxin by ZAP1 is an adaptive response to the oxidative stress of zinc deficiency. Abstract Text:

Chang-Yi Wu,Amanda J Bird,Dennis R Winge,David J Eide,

Zinc deficiency is a potential risk factor for disease in humans because it leads to increased oxidative stress and DNA damage. We show here that the yeast Saccharomyces cerevisiae also experiences oxidative stress when zinc-deficient, and we have identified one mechanism yeast cells use to defend themselves against this stress. The Zap1p transcription factor is a central player in the response of yeast to zinc deficiency. To identify genes important for growth in low zinc, DNA microarrays were used to identify genes directly regulated by Zap1p. We found that the TSA1 gene is one such Zap1p target whose expression is increased under zinc deficiency. TSA1 encodes a cytosolic thioredoxin-dependent peroxidase responsible for degrading hydrogen peroxide and organic hydroperoxides. Consistent with its regulation by Zap1p, we showed that tsa1delta mutants have a growth defect in low zinc that can be suppressed by zinc but not by other metals. Anaerobic conditions also suppressed the tsa1delta low zinc growth defect indicating that oxidative stress is the likely cause of the poor growth. Consistent with this hypothesis, we demonstrated that zinc deficiency causes increased reactive oxygen species in wild type cells and that this increase is further exacerbated in tsa1delta mutants. The role of this regulation by Zap1p in limiting oxidative stress in low zinc was confirmed when the Zap1p-binding site was specifically mutated in the chromosomal TSA1 promoter. Thus, we conclude that TSA1 induction by Zap1p is an adaptive response to deal with the increased oxidative stress caused by zinc deficiency.

Regulation of the yeast TSA1 peroxiredoxin by ZAP1 is an adaptive response to the oxidative stress of zinc deficiency. Publishing Authors By Initials

CY Wu,AJ Bird,DR Winge,DJ Eide,

For similar inorganic chemicals: elements: metals, heavy: zinc research abstracts see: inorganic chemicals: elements: metals, heavy: zinc research

PUBMED ID PMID:

MEDLINE DATE:

Regulation of the yeast TSA1 peroxiredoxin by ZAP1 is an adaptive response to the oxidative stress of zinc deficiency. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 2184-95

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 22

MONTH: 11

YEAR: 2006

Regulation of the yeast TSA1 peroxiredoxin by ZAP1 is an adaptive response to the oxidative stress of zinc deficiency. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Regulation of the yeast TSA1 peroxiredoxin by ZAP1 is an adaptive response to the oxidative stress of zinc deficiency. Keywords Mesh Terms:

KEYWORDS: Zinc

MESH TERMS: metabolism

Chemical & Substance for Abstract: Regulation of the yeast TSA1 peroxiredoxin by ZAP1 is an adaptive response to the oxidative stress of zinc deficiency. Information

Substance Name: Tsa1 protein, S cerevisiae

Registry Number: EC 1.11.1.-

Grant and Affiliation Information for Regulation of the yeast TSA1 peroxiredoxin by ZAP1 is an adaptive response to the oxidative stress of zinc deficiency.

AFFILIATION: Department of Nutritional Sciences, University of Wisconsin, Madison, Wisconsin 53706, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM56285

ACRONYM: GM

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Regulation of the yeast TSA1 peroxiredoxin by ZAP1 is an adaptive response to the oxidative stress of zinc deficiency Related Publications

• A histochemical study of the oxidation of 17 -estradiol in human term placenta at different incubation conditions. An investigation with the polyvinyl alcohol method.
• Acute disseminated phycomycosis in a patient with impaired neutrophil granulocyte function.
• Attributable fractions with survival data.
• Biometric measurements of the eyes in teenagers and young adults with Down syndrome.
• Comparison of simultaneous continuous intracranial pressure (ICP) signals from ICP sensors placed within the brain parenchyma and the epidural space.
• Distribution of atherosclerosis in human descending thoracic aorta. A morphometric study.
• HEMATOLOGIC AND CYTOGENIC EVIDENCE FOR CHIMERISM IN THE MARMOSET, TAMARINUS NIGRICOLLIS. SAM-TDR-64-61.
• Letter: Malignant carcinoid tumour with gangrene of the small intestine.
• Mutations in the RAD27 and SGS1 genes differentially affect the chronological and replicative lifespan of yeast cells growing on glucose and glycerol.
• Prevalence of probable idiopathic normal pressure hydrocephalus in a Norwegian population.
• Primary laser photocoagulation of "small" choroidal melanomas.
• Regulation of the yeast TSA1 peroxiredoxin by ZAP1 is an adaptive response to the oxidative stress of zinc deficiency.
• The frequency domain versus time domain methods for processing of intracranial pressure (ICP) signals.
• The probability of overlooking prostatic cancer in transurethrally resected material when different embedding practices are followed.
• [Angioimmunoblastic lymphadenopathy with dysproteinemia. A case report and a review of the literature]
• [Aspiration cytology of the breast]
• [Effect of physical activity on emotional reactions, reactions to stress and related physiologic reactions]
• [Epilepsy and the driving license]
• [Glossopharyngeal neuralgia]
• [Health education in the communities. A survey conducted in 1987]
• [Humanity and biotechnology--hearing statement of the Medical Society]
• [In Process Citation]
• [Meckel's diverticulum]
• [Muscle biopsies. Examination with computer-assisted morphometry]
• [Occurrence of recurrence after regression of low-grade dysplasia in vaginal cytological smears]
• [Pain surgery seen from the physiologic point of view]
• [Relations between body image, self concept and physical activity]
• [Salt and blood pressure in a male population from Sogn and Fjordane counties]
• [The Tallqvist hemoglobin scale -- a diagnostic aid?]
• [Use of hypnotics in geriatric institutions in Hordaland and Sogn and Fjordane]