Regulation of the Pro-apoptotic scaffolding protein POSH by Akt.

Regulation of the Pro-apoptotic scaffolding protein POSH by Akt. Research Abstract Details 

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Regulation of the Pro-apoptotic scaffolding protein POSH by Akt. Abstract Text:

Traci R Lyons,Jackie Thorburn,Philip W Ryan,Andrew Thorburn,Steven M Anderson,C Kenneth Kassenbrock,

POSH (Plenty of SH3 domains) binds to activated Rac and promotes apoptosis by acting as a scaffold to assemble a signal transduction pathway leading from Rac to JNK activation. Overexpression of POSH induces apoptosis in a variety of cell types, but apoptosis can be prevented by co-expressing the pro-survival protein kinase Akt. We report here that POSH is a direct substrate for phosphorylation by Akt in vivo and in vitro, and we identify a major site of Akt phosphorylation as serine 304 of POSH, which lies within the Rac-binding domain. We further show that phosphorylation of POSH results in a decreased ability to bind activated Rac, as does phosphomimetic S304D and S304E mutation of POSH. S304D mutant POSH also shows a strongly reduced ability to induce apoptosis. These findings identify a novel mechanism by which Akt promotes cell survival.

Regulation of the Pro-apoptotic scaffolding protein POSH by Akt. Publishing Authors By Initials

TR Lyons,J Thorburn,PW Ryan,A Thorburn,SM Anderson,CK Kassenbrock,

For similar enzymes and coenzymes: enzymes: ligases: ubiquitin-protein ligase complexes: ubiquitin-protein ligases research abstracts see: enzymes and coenzymes: enzymes: ligases: ubiquitin-protein ligase complexes: ubiquitin-protein ligases research

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Regulation of the Pro-apoptotic scaffolding protein POSH by Akt. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 21987-97

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 29

MONTH: 05

YEAR: 2007

Regulation of the Pro-apoptotic scaffolding protein POSH by Akt. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Regulation of the Pro-apoptotic scaffolding protein POSH by Akt. Keywords Mesh Terms:

KEYWORDS: Ubiquitin-Protein Ligases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Regulation of the Pro-apoptotic scaffolding protein POSH by Akt. Information

Substance Name: Ubiquitin-Protein Ligases

Registry Number: EC 6.3.2.19

Grant and Affiliation Information for Regulation of the Pro-apoptotic scaffolding protein POSH by Akt.

AFFILIATION: Department of Pathology, the University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK063674

ACRONYM: DK

MEDLINETA: J Biol Chem

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