Regulation of the prepronociceptin gene and its effect on neuronal differentiation.

Regulation of the prepronociceptin gene and its effect on neuronal differentiation. Research Abstract Details 

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Regulation of the prepronociceptin gene and its effect on neuronal differentiation. Abstract Text:

Naunihal T Zaveri,Nahid Waleh,Lawrence Toll,

Nociceptin/orphanin FQ (NOP/OFQ) is the endogenous ligand for the NOP receptor and is processed from a precursor protein in the family of opioid peptides. Prepronociceptin (ppN/OFQ) mRNA has been shown to be upregulated by an increase in cAMP, a treatment that leads to differentiation of NS20Y neuroblastoma cells. Although a large increase in endogenous ppN/OFQ mRNA upon cAMP stimulation can be shown in cellular systems, a similar increase cannot be expressed in pGL3 luciferase vector containing 1.3 kb proximal promoter, suggesting that a larger portion of the sequence or a different chromatin structure is necessary for a fully functional promoter. The induction of ppN/OFQ mRNA by cAMP appears to be mediated by a cAMP-response element. Chromatin immunoprecipitation (ChIP) assays show that CREB is recruited to the promoter region upon treatment of NS20Y cells with dibutyryl cAMP. In addition, the production of ppN/OFQ mRNA is regulated by histone acetylation, also through CREB, as the histone deacetylase (HDAC) inhibitor trichostatin A increases both CREB binding to the promoter and ppN/OFQ mRNA expression. In rat progenitor and mouse neuroblastoma cell lines, agents that increase ppN/OFQ mRNA expression also induce neurite outgrowth, suggesting a close relationship between ppN/OFQ and cellular differentiation.

Regulation of the prepronociceptin gene and its effect on neuronal differentiation. Publishing Authors By Initials

NT Zaveri,N Waleh,L Toll,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

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Regulation of the prepronociceptin gene and its effect on neuronal differentiation. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Gene

VOLUME: 384

Page Numbers: 27-36

Journal Abbreviation: Gene

ISSN: 0378-1119

DAY: 20

MONTH: 07

YEAR: 2006

Regulation of the prepronociceptin gene and its effect on neuronal differentiation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7706761

Regulation of the prepronociceptin gene and its effect on neuronal differentiation. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: drug effects

Chemical & Substance for Abstract: Regulation of the prepronociceptin gene and its effect on neuronal differentiation. Information

Substance Name: mithramycin A

Registry Number: 97666-60-9

Grant and Affiliation Information for Regulation of the prepronociceptin gene and its effect on neuronal differentiation.

AFFILIATION: SRI International, Biosciences Division, 333 Ravenswood Ave, LA214A, Menlo Park, CA 94025, USA. naunihal.zaveri@sri.com

Country: Netherlands

AGENCY: United States NIDA

GRANT: DA06682

ACRONYM: DA

MEDLINETA: Gene

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