Regulation of the M-cadherin-beta-catenin complex by calpain 3 during terminal stages of myogenic differentiation.

Regulation of the M-cadherin-beta-catenin complex by calpain 3 during terminal stages of myogenic differentiation. Research Abstract Details 

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Regulation of the M-cadherin-beta-catenin complex by calpain 3 during terminal stages of myogenic differentiation. Abstract Text:

Irina Kramerova,Elena Kudryashova,Benjamin Wu,Melissa J Spencer,

The cysteine protease calpain 3 (CAPN3) is essential for normal muscle function, since mutations in CAPN3 cause limb girdle muscular dystrophy type 2A. Previously, we showed that myoblasts isolated from CAPN3 knockout (C3KO) mice were able to fuse to myotubes; however, sarcomere formation was disrupted. In this study we further characterized morphological and biochemical features of C3KO myotubes in order to elucidate a role for CAPN3 during myogenesis. We showed that cell cycle withdrawal occurred normally in C3KO cultures, but C3KO myotubes have an increased number of myonuclei per myotube. We found that CAPN3 acts during myogenesis to specifically control levels of membrane-associated but not cytoplasmic beta-catenin and M-cadherin. CAPN3 was able to cleave both proteins, and in the absence of CAPN3, M-cadherin and beta-catenin abnormally accumulated at the membranes of myotubes. Given the role of M-cadherin in myoblast fusion, this finding suggests that the excessive myonuclear index of C3KO myotubes was due to enhanced fusion. Postfusion events, such as beta1D integrin expression and myofibrillogenesis, were suppressed in C3KO myotubes. These data suggest that the persistence of fusion observed in C3KO cells inhibits subsequent steps of differentiation, such as integrin complex rearrangements and sarcomere assembly.

Regulation of the M-cadherin-beta-catenin complex by calpain 3 during terminal stages of myogenic differentiation. Publishing Authors By Initials

I Kramerova,E Kudryashova,B Wu,MJ Spencer,

For similar proteins: armadillo domain proteins: beta catenin research abstracts see: proteins: armadillo domain proteins: beta catenin research

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Regulation of the M-cadherin-beta-catenin complex by calpain 3 during terminal stages of myogenic differentiation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular and cellular biology

VOLUME: 26

Page Numbers: 8437-47

Journal Abbreviation: Mol. Cell. Biol.

ISSN: 0270-7306

DAY: 18

MONTH: 09

YEAR: 2006

Regulation of the M-cadherin-beta-catenin complex by calpain 3 during terminal stages of myogenic differentiation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8109087

Regulation of the M-cadherin-beta-catenin complex by calpain 3 during terminal stages of myogenic differentiation. Keywords Mesh Terms:

KEYWORDS: beta Catenin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Regulation of the M-cadherin-beta-catenin complex by calpain 3 during terminal stages of myogenic differentiation. Information

Substance Name: Capn3 protein, mouse

Registry Number: EC 3.4.22.-

Grant and Affiliation Information for Regulation of the M-cadherin-beta-catenin complex by calpain 3 during terminal stages of myogenic differentiation.

AFFILIATION: Department of Neurology, David Geffen School of Medicine, Neuroscience Research Building, University of California at Los Angeles, Los Angeles, CA 90095-7334, USA. ikramero@ucla.edu

Country: United States

AGENCY: United States NIAMS

GRANT: R01 AR48177

ACRONYM: AR

MEDLINETA: Mol Cell Biol

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