Regulation of the Inositol 1,4,5-Trisphosphate Receptor Type I by O-GlcNAc Glycosylation.

Regulation of the Inositol 1,4,5-Trisphosphate Receptor Type I by O-GlcNAc Glycosylation. Research Abstract Details 

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Regulation of the Inositol 1,4,5-Trisphosphate Receptor Type I by O-GlcNAc Glycosylation. Abstract Text:

Juliana Rengifo,Craig J Gibson,Eva Winkler,Thibault Collin,Barbara E Ehrlich,Juliana Rengifo,Craig J Gibson,Eva Winkler,Thibault Collin,Barbara E Ehrlich,Juliana Rengifo,Craig J Gibson,Eva Winkler,Thibault Collin,Barbara E Ehrlich,

The inositol 1,4,5-trisphosphate (InsP(3)) receptor type I (InsP(3)R-I) is the principle channel for intracellular calcium (Ca(2+)) release in many cell types, including central neurons. It is regulated by endogenous compounds like Ca(2+) and ATP, by protein partners, and by posttranslational modification. We report that the InsP(3)R-I is modified by O-linked glycosylation of serine or threonine residues with beta-N-acetylglucosamine (O-GlcNAc). The level of O-GlcNAcylation can be altered in vitro by the addition of the enzymes which add [OGT (O-GlcNActransferase)] or remove (O-GlcNAcase) this sugar or by loading cells with UDP-GlcNAc. We monitored the effects of this modification on InsP(3)R function at the single-channel level and on intracellular Ca(2+) transients. Single-channel activity was monitored with InsP(3)R incorporated into bilayers; Ca(2+) signaling was monitored using cells loaded with a Ca(2+)-sensitive fluorophore. We found that channel activity was decreased by the addition of O-GlcNAc and that this decrease was reversed by removal of the sugar. Similarly, cells loaded with UDP-GlcNAc had an attenuated response to uncaging of InsP(3). These results show that O-GlcNAcylation is an important regulator of the InsP(3)R-I and suggest a mechanism for neuronal dysfunction under conditions in which O-GlcNAc is high, such as diabetes or physiological stress.

Regulation of the Inositol 1,4,5-Trisphosphate Receptor Type I by O-GlcNAc Glycosylation. Publishing Authors By Initials

J Rengifo,CJ Gibson,E Winkler,T Collin,BE Ehrlich,J Rengifo,CJ Gibson,E Winkler,T Collin,BE Ehrlich,J Rengifo,CJ Gibson,E Winkler,T Collin,BE Ehrlich,

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Regulation of the Inositol 1,4,5-Trisphosphate Receptor Type I by O-GlcNAc Glycosylation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of neuroscience : the official journal

VOLUME: 27

Page Numbers: 13813-21

Journal Abbreviation: J. Neurosci.

ISSN: 1529-2401

DAY: 12

MONTH: Dec

YEAR: 2007

Regulation of the Inositol 1,4,5-Trisphosphate Receptor Type I by O-GlcNAc Glycosylation. Information

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LANGUAGE: eng

NlmUniqueID: 8102140

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AFFILIATION: Department of Pharmacology and Cellular and Molecular Physiology, Yale University, New Haven, Connecticut 06520, and Laboratoire de Physiologie Cérébrale, Université Paris 5, 75006 Paris, France.

Country: United States

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MEDLINETA: J Neurosci

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