Regulation of period 1 expression in cultured rat pineal.

Regulation of period 1 expression in cultured rat pineal. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Regulation of period 1 expression in cultured rat pineal. Abstract Text:

Chiaki Fukuhara,James C Dirden,Gianluca Tosini,

The aim of the present study was to investigate the in vitro expression of Period 1 (Per1), Period 2 (Per2) and arylalkylamine N-acetyltransferase (AA-NAT) genes in the rat pineal gland to understand the mechanism(s) regulating the expression of these genes in this organ. Pineals, when maintained in vitro for 5 days, did not show circadian rhythmicity in the expression of any of the three genes monitored. Norepinephrine (NE) induced AA-NAT and Per1, whereas its effect on Per2 was negligible. Contrary to what was observed in other systems, NE stimulation did not induce circadian expression of Per1. The effect of NE on Per1 level was dose- and receptor subtype-dependent, and both cAMP and cGMP induced Per1. Per1 was not induced by repeated NE - or forskolin - stimulation. Protein synthesis was not necessary for NE-induced Per1, but it was for reduction of Per1 following NE stimulation. Per1 transcription in pinealocytes was activated by BMAL1/CLOCK. Our results indicate that important differences are present in the regulation of these genes in the mammalian pineal.

Regulation of period 1 expression in cultured rat pineal. Publishing Authors By Initials

C Fukuhara,JC Dirden,G Tosini,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

PUBMED ID PMID:

MEDLINE DATE: 2002 Mar-Apr

Regulation of period 1 expression in cultured rat pineal. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Neuro-Signals

VOLUME: 11

Page Numbers: 103-14

Journal Abbreviation: Neurosignals

ISSN: 1424-862X

DAY: 19

MONTH: 11

YEAR: 2007

Regulation of period 1 expression in cultured rat pineal. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101134359

Regulation of period 1 expression in cultured rat pineal. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: physiology

Chemical & Substance for Abstract: Regulation of period 1 expression in cultured rat pineal. Information

Substance Name: Arylamine N-Acetyltransferase

Registry Number: EC 2.3.1.5

Grant and Affiliation Information for Regulation of period 1 expression in cultured rat pineal.

AFFILIATION: Morehouse Sch Med, Atlanta, GA

Country: Switzerland

AGENCY: United States NINDS

GRANT: NS 38483

ACRONYM: NS

MEDLINETA: Neurosignals

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Regulation of period 1 expression in cultured rat pineal Related Publications

• Analysis of daily and circadian gene expression in the rat pineal gland.
• Circadian expression of period 1, period 2, and arylalkylamine N-acetyltransferase mRNA in the rat pineal gland under different light conditions.
• Regulation of period 1 expression in cultured rat pineal.