Regulation of nif gene expression and the energetics of N(2) fixation over the diel cycle in a hot spring microbial mat.

Regulation of nif gene expression and the energetics of N(2) fixation over the diel cycle in a hot spring microbial mat. Research Abstract Details 

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Regulation of nif gene expression and the energetics of N(2) fixation over the diel cycle in a hot spring microbial mat. Abstract Text:

Nitrogen fixation, a prokaryotic, O(2)-inhibited process that reduces N(2) gas to biomass, is of paramount importance in biogeochemical cycling of nitrogen. We analyzed the levels of nif transcripts of Synechococcus ecotypes, NifH subunit and nitrogenase activity over the diel cycle in the microbial mat of an alkaline hot spring in Yellowstone National Park. The results showed a rise in nif transcripts in the evening, with a subsequent decline over the course of the night. In contrast, immunological data demonstrated that the level of the NifH polypeptide remained stable during the night, and only declined when the mat became oxic in the morning. Nitrogenase activity was low throughout the night; however, it exhibited two peaks, a small one in the evening and a large one in the early morning, when light began to stimulate cyanobacterial photosynthetic activity, but O(2) consumption by respiration still exceeded the rate of O(2) evolution. Once the irradiance increased to the point at which the mat became oxic, the nitrogenase activity was strongly inhibited. Transcripts for proteins associated with energy-producing metabolisms in the cell also followed diel patterns, with fermentation-related transcripts accumulating at night, photosynthesis- and respiration-related transcripts accumulating during the day and late afternoon, respectively. These results are discussed with respect to the energetics and regulation of N(2) fixation in hot spring mats and factors that can markedly influence the extent of N(2) fixation over the diel cycle.The ISME Journal (2008) 2, 364-378; doi:10.1038/ismej.2007.117; published online 6 March 2008.

Regulation of nif gene expression and the energetics of N(2) fixation over the diel cycle in a hot spring microbial mat. Publishing Authors By Initials

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Regulation of nif gene expression and the energetics of N(2) fixation over the diel cycle in a hot spring microbial mat. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The ISME journal

VOLUME: 2

Page Numbers: 364-78

Journal Abbreviation: ISME J

ISSN: 1751-7362

DAY: 6

MONTH: 03

YEAR: 2008

Regulation of nif gene expression and the energetics of N(2) fixation over the diel cycle in a hot spring microbial mat. Information

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LANGUAGE: eng

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AFFILIATION: 1Department of Plant Biology, The Carnegie Institution, Stanford University, Stanford, CA, USA.

Country: England

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MEDLINETA: ISME J

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