Regulation of interferon-stimulated genes in peripheral blood leukocytes in pregnant and bred, nonpregnant dairy cows.

Regulation of interferon-stimulated genes in peripheral blood leukocytes in pregnant and bred, nonpregnant dairy cows. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Regulation of interferon-stimulated genes in peripheral blood leukocytes in pregnant and bred, nonpregnant dairy cows. Abstract Text:

C A Gifford,K Racicot,D S Clark,K J Austin,T R Hansen,M C Lucy,C J Davies,T L Ott,

In ruminants, pregnancy results in up-regulation of a large number of IFN-stimulated genes (ISG) in the uterus. Recently, one of these genes was also shown to increase in peripheral blood leukocytes (PBL) during early pregnancy in sheep. Our working hypothesis is that conceptus signaling activates maternal gene expression in PBL in dairy cattle. The objectives of this study were to characterize ISG expression in PBL from pregnant (n = 20) and bred, nonpregnant (n = 30) dairy cows. Steady-state levels of mRNA for Mx1, Mx2, beta2-microglobulin, ISG-15, IFN regulatory factor-1, and IFN regulatory factor-2 were quantified. Holstein cows were synchronized to estrus and artificially inseminated (d 0). Blood samples were collected (coccygeal venipuncture) on d 0 and 16, 18, and 20 d after insemination for progesterone analysis and PBL isolation. Pregnancy was confirmed by transrectal ultrasonography at approximately 40 d after breeding. A status x day interaction was detected for Mx1, Mx2, and ISG-15 gene expression. When analyzed within day, levels of mRNA for ISG-15 and Mx1 were greater in pregnant compared with bred, nonpregnant cows on d 18 and 20, respectively. Expression of the Mx2 gene increased in the pregnant group compared with bred, nonpregnant cows on d 16, 18, and 20 after insemination. beta2-Microglobulin, IFN regulatory factor-1, and IFN regulatory factor-2 were not different between groups. The results clearly indicated that components of the innate immune response are activated in PBL during the period of pregnancy recognition and early embryo signaling. The physiological implications of these changes on maternal immune function are as yet unknown; however, they do provide a unique opportunity to identify bred, nonpregnant, cows 18 d after insemination in dairy cattle.

Regulation of interferon-stimulated genes in peripheral blood leukocytes in pregnant and bred, nonpregnant dairy cows. Publishing Authors By Initials

CA Gifford,K Racicot,DS Clark,KJ Austin,TR Hansen,MC Lucy,CJ Davies,TL Ott,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Regulation of interferon-stimulated genes in peripheral blood leukocytes in pregnant and bred, nonpregnant dairy cows. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of dairy science

VOLUME: 90

Page Numbers: 274-80

Journal Abbreviation: J. Dairy Sci.

ISSN: 1525-3198

DAY: 21

MONTH: Jan

YEAR: 2007

Regulation of interferon-stimulated genes in peripheral blood leukocytes in pregnant and bred, nonpregnant dairy cows. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985126

Regulation of interferon-stimulated genes in peripheral blood leukocytes in pregnant and bred, nonpregnant dairy cows. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Regulation of interferon-stimulated genes in peripheral blood leukocytes in pregnant and bred, nonpregnant dairy cows. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Regulation of interferon-stimulated genes in peripheral blood leukocytes in pregnant and bred, nonpregnant dairy cows.

AFFILIATION: Department of Animal and Veterinary Sciences, University of Idaho, Moscow 83844, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Dairy Sci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Regulation of interferon-stimulated genes in peripheral blood leukocytes in pregnant and bred, nonpregnant dairy cows Related Publications

• 1009.
• A quantitative assay method for pyrogens.
• Assessing blood flow with CT angiography.
• Cellular localization and function of the antiviral protein, ovine Mx1 (oMx1): I. Ovine Mx1 is secreted by endometrial epithelial cells via an 'unconventional' secretory pathway.
• Cellular localization and function of the antiviral protein, ovine Mx1 (oMx1): II. The oMx1 protein is a regulator of secretion in an ovine glandular epithelial cell line.
• Cloning and characterizing of the ovine MX1 gene promoter/enhancer region.
• Esophageal cancer.
• Genetic risk factors for diabetic nephropathy on chromosomes 6p and 7q identified by the set-association approach.
• Genome-wide annotation of remorins, a plant-specific protein family: evolutionary and functional perspectives.
• Incidence and treatments of postpartum reproductive problems in a dairy herd.
• Metabolism of Reactive Oxygen Species Is Attenuated in Leghemoglobin-Deficient Nodules of Lotus japonicus.
• Mucin coatings suppress neutrophil adhesion to a polymeric model biomaterial.
• Mutational analysis of the C-terminal gag cleavage sites in human immunodeficiency virus type 1.
• Office treatment of lesions of the cervix.
• Regulation of interferon-stimulated genes in peripheral blood leukocytes in pregnant and bred, nonpregnant dairy cows.
• Reviews in medical ethics. One goal? One consensus? One more trip to the drawing board: a review of global bioethics: the collapse of consensus.
• Sonographic diagnosis of fetal growth restriction.
• Stem cells in liver regeneration and therapy.
• Studying host-pathogen interactions in 3-D: organotypic models for infectious disease and drug development.
• The effect of short term calf removal in combination with GnRH treatment and the effect of limited nursing on reproductive performance of postpartum suckled beef cows administered PGF(2)alpha for ovulation control.
• The impact of sulfonamide and antibiotic agents on the prognosis in infectious diseases.
• Visual and cognitive predictors of driving safety in Parkinson's disease patients.
• [Fifty years of the discovery of radium.]
• [In Process Citation]
• [Modern endoscopic procedures for thoracic diseases]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [The effect of light on the vitamin content in milk.]