Regulation of heme oxygenase-1 expression by demethoxy curcuminoids through Nrf2 by a PI3-kinase/Akt-mediated pathway in mouse beta-cells.

Regulation of heme oxygenase-1 expression by demethoxy curcuminoids through Nrf2 by a PI3-kinase/Akt-mediated pathway in mouse beta-cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Regulation of heme oxygenase-1 expression by demethoxy curcuminoids through Nrf2 by a PI3-kinase/Akt-mediated pathway in mouse beta-cells. Abstract Text:

Subbiah Pugazhenthi,Leonid Akhov,Gopalan Selvaraj,Maorong Wang,Jawed Alam,

Curcumin (diferuloylmethane), a component of turmeric, has been shown to have therapeutic properties. Induction of phase 2 detoxifying enzymes is a potential mechanism through which some of the actions of curcumin could proceed. Heme oxygenase-1 (HO-1), an antioxidant phase 2 enzyme, has been reported to have cytoprotective effects in pancreatic beta-cells. Curcumin on further purification yields demethoxy curcumin (DMC) and bisdemethoxy curcumin (BDMC). The objective of the present study was to determine the mechanism by which these purified curcuminoids induce HO-1 in MIN6 cells, a mouse beta-cell line. Demethoxy curcuminoids induced HO-1 promoter linked to the luciferase reporter gene more effectively than curcumin. The induction was dependent on the presence of antioxidant response element (ARE) sites containing enhancer regions (E1 and E2) in HO-1 promoter and nuclear translocation of nuclear factor-E2-related factor (Nrf2), the transcription factor that binds to ARE. Curcuminoids stimulated multiple signaling pathways that are known to induce HO-1. Inhibition of specific signaling pathways with pharmacological inhibitors and cotransfection experiments suggested the involvement of phosphotidylinositol 3-kinase and Akt. Real-time quantitative RT-PCR analysis showed significant elevation in the mRNA levels of HO-1 and two other phase 2 enzymes, the regulatory subunit of glutamyl cysteine ligase, which is needed for the synthesis of glutathione, and NAD(P)H:quinone oxidoreductase, which detoxifies quinones. DMC and BDMC induced the expression of HO-1 and translocated Nrf2 to nucleus in beta-cells of mouse islets. Our observations suggest that demethoxy curcuminoids could be used to induce a cellular defense mechanism in beta-cells under conditions of stress as seen in diabetes.

Regulation of heme oxygenase-1 expression by demethoxy curcuminoids through Nrf2 by a PI3-kinase/Akt-mediated pathway in mouse beta-cells. Publishing Authors By Initials

S Pugazhenthi,L Akhov,G Selvaraj,M Wang,J Alam,

For similar biological phenomena, cell phenomena, and immunity: cell physiology: cell communication: signal transduction research abstracts see: biological phenomena, cell phenomena, and immunity: cell physiology: cell communication: signal transduction research

PUBMED ID PMID:

MEDLINE DATE:

Regulation of heme oxygenase-1 expression by demethoxy curcuminoids through Nrf2 by a PI3-kinase/Akt-mediated pathway in mouse beta-cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of physiology. Endocrinology and

VOLUME: 293

Page Numbers: E645-55

Journal Abbreviation: Am. J. Physiol. Endocrinol. Me

ISSN: 0193-1849

DAY: 29

MONTH: 05

YEAR: 2007

Regulation of heme oxygenase-1 expression by demethoxy curcuminoids through Nrf2 by a PI3-kinase/Akt-mediated pathway in mouse beta-cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901226

Regulation of heme oxygenase-1 expression by demethoxy curcuminoids through Nrf2 by a PI3-kinase/Akt-mediated pathway in mouse beta-cells. Keywords Mesh Terms:

KEYWORDS: Signal Transduction

MESH TERMS: physiology

Chemical & Substance for Abstract: Regulation of heme oxygenase-1 expression by demethoxy curcuminoids through Nrf2 by a PI3-kinase/Akt-mediated pathway in mouse beta-cells. Information

Substance Name: Proto-Oncogene Proteins c-akt

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Regulation of heme oxygenase-1 expression by demethoxy curcuminoids through Nrf2 by a PI3-kinase/Akt-mediated pathway in mouse beta-cells.

AFFILIATION: Department of Medicine, University of Colorado at Denver and Health Sciences Center, P.O. Box 6511, Mail Stop 8106, Aurora, CO 80045, USA. subbiah.pugazhenthi@uchsc.edu

Country: United States

AGENCY: United States NIDDK

GRANT: P30 DK-057516

ACRONYM: DK

MEDLINETA: Am J Physiol Endocrinol Metab

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Regulation of heme oxygenase-1 expression by demethoxy curcuminoids through Nrf2 by a PI3-kinase/Akt-mediated pathway in mouse beta-cells Related Publications

• 17O NMR investigation of phosphite hydrolysis mechanisms.
• Assessment of indicators for predicting choledocholithiasis before laparoscopic cholecystectomy.
• Breast cancer in pakistan - awareness and early detection.
• Differential effect of resuscitation on Toll-like receptors in a model of hemorrhagic shock without a septic challenge.
• Dimensionally Frustrated Diffusion towards Fractal Adsorbers.
• Effects of serotonin on perifornical-lateral hypothalamic area neurons in rat.
• Epidemiology of hydatid disease in Riyadh: A hospital-based study.
• Foreign body in submandibular region.
• Foreword biomarkers in asthma and allergy: are we there yet?
• Genetic Structure of Different Populations of Walking Catfish (Clarias batrachus L.) in Bangladesh.
• HIV-1 Hides an Achilles' Heel in Virion Lipids.
• Hypercholesterolemia in healthy adult males - A public survey in central Saudi Arabia.
• Intermittent oral versus intravenous alfacalcidol in dialysis patients.
• Methane oxidation by an extremely acidophilic bacterium of the phylum Verrucomicrobia.
• Microfilariae in thyroid aspiration smear-An unexpected finding.
• Natural orifice surgery - is it 'big thing' ?
• Pancreatic pseudocyst: A review of 5 years' experience.
• Phase 2 study of the combination of merimepodib with peginterferon-alpha2b, and ribavirin in nonresponders to previous therapy for chronic hepatitis C.
• Profound hypothermic cardiopulmonary bypass facilitates survival without a high complication rate in a swine model of complex vascular, splenic, and colon injuries.
• Quercus infectoria galls possess antioxidant activity and abrogates oxidative stress-induced functional alterations in murine macrophages.
• Regulation of heme oxygenase-1 expression by demethoxy curcuminoids through Nrf2 by a PI3-kinase/Akt-mediated pathway in mouse beta-cells.
• Synthesis of organo-silane functionalized nanocrystal micelles and their self-assembly.
• The prolactin family: effectors of pregnancy-dependent adaptations.
• The role of ERCP in the management of gallstone-associated acute pancreatitis: A prospective study of 64 patients at Riyadh Central Hospital.
• Thyroid hemiagenesis: Case report and review of the literature.
• Training in reference management software--a part of new medical informatics workshops in Pakistan.
• Unc119 regulates myofibroblast differentiation through the activation of Fyn and the p38 MAPK pathway.
• Using (31)P MAS NMR to monitor a gel phase thermal disorder transition in sphingomyelin/cholesterol bilayers.
• Using mushroom farm and anaerobic digestion wastewaters as supplemental fertilizer sources for growing container nursery stock in a closed system.
• Video-assisted thoracic surgery lobectomy (VATS), open thoracotomy, and the robot for lung cancer.