Regulation of D-xylose metabolism in Caulobacter crescentus by a LacI-type repressor.

Regulation of D-xylose metabolism in Caulobacter crescentus by a LacI-type repressor. Research Abstract Details 

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Regulation of D-xylose metabolism in Caulobacter crescentus by a LacI-type repressor. Abstract Text:

Craig Stephens,Beat Christen,Kelly Watanabe,Thomas Fuchs,Urs Jenal,Craig Stephens,Beat Christen,Kelly Watanabe,Thomas Fuchs,Urs Jenal,Craig Stephens,Beat Christen,Kelly Watanabe,Thomas Fuchs,Urs Jenal,

In the oligotrophic freshwater bacterium Caulobacter crescentus, D-xylose induces expression of over 50 genes, including the xyl operon, which encodes key enzymes for xylose metabolism. The promoter (P(xylX)) controlling expression of the xyl operon is widely used as a tool for inducible heterologous gene expression in C. crescentus. We show here that P(xylX) and at least one other promoter in the xylose regulon (P(xylE)) are controlled by the CC3065 (xylR) gene product, a LacI-type repressor. Electrophoretic gel mobility shift assays showed that operator binding by XylR is greatly reduced in the presence of D-xylose. The data support the hypothesis that there is a simple regulatory mechanism in which XylR obstructs xylose-inducible promoters in the absence of the sugar; the repressor is induced to release DNA upon binding D-xylose, thereby freeing the promoter for productive interaction with RNA polymerase. XylR also has an effect on glucose metabolism, as xylR mutants exhibit reduced expression of the Entner-Doudoroff operon and their ability to utilize glucose as a sole carbon and energy source is compromised.

Regulation of D-xylose metabolism in Caulobacter crescentus by a LacI-type repressor. Publishing Authors By Initials

C Stephens,B Christen,K Watanabe,T Fuchs,U Jenal,C Stephens,B Christen,K Watanabe,T Fuchs,U Jenal,C Stephens,B Christen,K Watanabe,T Fuchs,U Jenal,

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Regulation of D-xylose metabolism in Caulobacter crescentus by a LacI-type repressor. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of bacteriology

VOLUME: 189

Page Numbers: 8828-34

Journal Abbreviation: J. Bacteriol.

ISSN: 1098-5530

DAY: 12

MONTH: 10

YEAR: 2007

Regulation of D-xylose metabolism in Caulobacter crescentus by a LacI-type repressor. Information

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LANGUAGE: eng

NlmUniqueID: 2985120

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AFFILIATION: Biology Department, Santa Clara University, 500 El Camino Real, Santa Clara, CA 95053, USA. CStephens@scu.edu

Country: United States

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MEDLINETA: J Bacteriol

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