Regulation of cGMP-dependent protein kinase-mediated vasodilation by hypoxia-induced reactive species in ovine fetal pulmonary veins.

Regulation of cGMP-dependent protein kinase-mediated vasodilation by hypoxia-induced reactive species in ovine fetal pulmonary veins. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Regulation of cGMP-dependent protein kinase-mediated vasodilation by hypoxia-induced reactive species in ovine fetal pulmonary veins. Abstract Text:

Sewite Negash,Yuansheng Gao,Weilin Zhou,Jie Liu,Shashi Chinta,J Usha Raj,Sewite Negash,Yuansheng Gao,Weilin Zhou,Jie Liu,Shashi Chinta,J Usha Raj,

We previously reported that hypoxia attenuates cGMP-dependent protein kinase (PKG)-mediated relaxation in pulmonary vessels (Am J Physiol Lung Cell Mol Physiol 279: L611-L618, 2003). To determine whether hypoxia-induced reactive oxygen and nitrogen species (ROS and RNS, respectively) may be involved in the downregulation of PKG-mediated relaxation, ovine fetal intrapulmonary veins were exposed to 4 h of normoxia or hypoxia, with or without scavengers of ROS [N-acetylcysteine (NAC)] or peroxynitrite (quercetin and Trolox) and preconstricted with endothelin-1. Hypoxia decreased the relaxation response to 8-bromo-cGMP, PKG protein expression, and kinase activity and increased tyrosine nitration in PKG. However, ROS and RNS scavengers prevented these changes. To determine whether increased PKG nitration diminishes PKG activity, pulmonary vein smooth muscle cells (PVSMC) were exposed to shorter-term (30 min) hypoxia, which increased PKG nitration and decreased PKG activity but did not alter PKG protein expression. Increased dihydro-2,7-dichlorofluorescein diacetate (DCFH(2)-DA) fluorescence in PVSMC after 4 h or 30 min of hypoxia was not observed in the presence of NAC, quercetin, or Trolox, suggesting increased ROS and RNS production. Increased PKG nitration and the associated decrease in PKG activity in PVSMC after 30 min of hypoxia were also reversed on reoxygenation. The consequences of PKG nitration were assessed by exposure of purified PKG-Ialpha to peroxynitrite, which caused increased 3-nitrotyrosine immunoreactivity and inhibition of kinase activity. Our data suggest that, after 30 min of hypoxia, reversible covalent modification of PKG by hypoxia-induced reactive species may be an important mechanism by which the relaxation response to cGMP is regulated. However, after 4 h of hypoxia, PKG nitration and decreased PKG expression are involved.

Regulation of cGMP-dependent protein kinase-mediated vasodilation by hypoxia-induced reactive species in ovine fetal pulmonary veins. Publishing Authors By Initials

S Negash,Y Gao,W Zhou,J Liu,S Chinta,JU Raj,S Negash,Y Gao,W Zhou,J Liu,S Chinta,JU Raj,

For similar vasodilation research abstracts see: vasodilation research

PUBMED ID PMID:

MEDLINE DATE:

Regulation of cGMP-dependent protein kinase-mediated vasodilation by hypoxia-induced reactive species in ovine fetal pulmonary veins. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: American journal of physiology. Lung cellular and

VOLUME: 293

Page Numbers: L1012-20

Journal Abbreviation: Am. J. Physiol. Lung Cell Mol.

ISSN: 1040-0605

DAY: 6

MONTH: 07

YEAR: 2007

Regulation of cGMP-dependent protein kinase-mediated vasodilation by hypoxia-induced reactive species in ovine fetal pulmonary veins. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901229

Regulation of cGMP-dependent protein kinase-mediated vasodilation by hypoxia-induced reactive species in ovine fetal pulmonary veins. Keywords Mesh Terms:

KEYWORDS: Vasodilation

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: Regulation of cGMP-dependent protein kinase-mediated vasodilation by hypoxia-induced reactive species in ovine fetal pulmonary veins. Information

Substance Name: cGMP-dependent protein kinase Ialpha

Registry Number: EC 2.7.10.-

Grant and Affiliation Information for Regulation of cGMP-dependent protein kinase-mediated vasodilation by hypoxia-induced reactive species in ovine fetal pulmonary veins.

AFFILIATION: Division of Neonatology, Harbor-UCLA Medical Center, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles Biomedical Research Institute, Torrance, California 90502, USA. snegash@labiomed.org

Country: United States

AGENCY: United States NHLBI

GRANT: R01/MIRS HL-75187-01

ACRONYM: HL

MEDLINETA: Am J Physiol Lung Cell Mol Phy

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Regulation of cGMP-dependent protein kinase-mediated vasodilation by hypoxia-induced reactive species in ovine fetal pulmonary veins Related Publications

• An unusual manifestation of Abrus precatorius poisoning: A report of two cases.
• Cardiopulmonary Stress Testing in Children With Sickle Cell Disease Who are on Long-term Erythrocytapheresis.
• Characterization of protein transacetylase from human placenta as a signaling molecule calreticulin using polyphenolic peracetates as the acetyl group donors.
• Coat protein-mediated resistance against an Indian isolate of the Cucumber mosaic virus subgroup IB in Nicotiana benthamiana.
• Comparative quantification of ingress of trypan blue into the anterior chamber after microcoaxial, standard coaxial, and bimanual phacoemulsification Randomized clinical trial.
• Dental surgeon and medical health.
• Eicosapentaenoic acid enrichment from sardine oil by argentation chromatography.
• Elevation of serum riboflavin carrier protein in hepatocellular carcinoma.
• Genetic polymorphisms of the RAS-cytokine pathway and chronic kidney disease.
• Humane killing of nonhuman animals for disease control purposes.
• In Vivo Evidence for the Role of CD44s in Promoting Breast Cancer Metastasis to the Liver.
• Ingress of bacterial inoculum into the anterior chamber after bimanual and microcoaxial phacoemulsification in rabbits.
• Intervertebral disc: anatomy-physiology-pathophysiology-treatment.
• Intimate partner violence around the time of pregnancy: association with breastfeeding behavior.
• Mercaptoethylpyrazine promoted electrochemistry of redox protein and amperometric biosensing of uric acid.
• Mitral valve repair in infants and children.
• Modulation of pulmonary vascular smooth muscle cell phenotype in hypoxia: role of cGMP-dependent protein kinase.
• Nodular secondary syphilis mimicking cutaneous lymphoreticular process.
• Optical sensing of biomedically important polyionic drugs using nano-sized gold particles.
• Platelet-activating factor stimulates ovine foetal pulmonary vascular smooth muscle cell proliferation: role of nuclear factor-kappa B and cyclin-dependent kinases.
• Protective mechanisms of p53-p21-pRb proteins against DNA damage-induced cell death.
• Regulation of cGMP-dependent protein kinase-mediated vasodilation by hypoxia-induced reactive species in ovine fetal pulmonary veins.
• Regulation of endothelial nitric oxide synthase: involvement of protein kinase G 1 beta, serine 116 phosphorylation and lipid structures.
• Renal Function Outcomes in Patients Treated for Renal Masses Smaller Than 4 cm by Ablative and Extirpative Techniques.
• Role of platelet-activating factor in pulmonary vascular remodeling associated with chronic high altitude hypoxia in ovine fetal lambs.
• Shipbreaking in the developing world: problems and prospects.
• Syphilis and Hepatitis B Co-infection among HIV-Infected, Sex-Trafficked Women and Girls, Nepal.
• Techniques used for the determination of blood volume.
• Topical Lomefloxacin versus Topical Chloramphenicol in the Treatment of Acute Bacterial Conjunctivitis.
• Topical beta blockers and serum lipoproteins.