Regulation of cell proliferation in a stratified culture system of epithelial cells from prostate tissue.

Regulation of cell proliferation in a stratified culture system of epithelial cells from prostate tissue. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Regulation of cell proliferation in a stratified culture system of epithelial cells from prostate tissue. Abstract Text:

Michael P Gustafson,Chang Xu,Jonathan E Grim,Bruce E Clurman,Beatrice S Knudsen,

Mechanisms controlling epithelial proliferation and differentiation in the prostate have been primarily investigated in mouse models. The regulation of proliferation and differentiation is poorly understood in human prostate epithelial cells. In vivo, the glandular prostate epithelium consists of a p63-positive proliferating basal cell layer and a post-mitotic p27-positive secretory cell layer. We have established an organized stratified culture system of human primary prostate epithelial cells to gain insight into mechanisms regulating proliferation and differentiation. In this system, expression of p63 is observed in the bottom layer. In addition, BrdU incorporation persists even though cells are confluent. In contrast, in the upper layer, p63 expression is greatly diminished, p27 is expressed, and the cells are growth arrested. Overexpression of cyclin D1 or knockdown of p27 does not increase proliferation. After inactivation of the nuclear phosphoprotein Rb, the cell layers remain organized and cell proliferation increases only in the bottom layer. Furthermore, the expression of p63 remains confined to the bottom layer after Rb inactivation. Altogether, this in vitro model recapitulates certain aspects of in vivo growth regulation and differentiation and suggests that the loss of Rb family proteins in human cells trigger hyperplasia but is not sufficient for transformation.

Regulation of cell proliferation in a stratified culture system of epithelial cells from prostate tissue. Publishing Authors By Initials

MP Gustafson,C Xu,JE Grim,BE Clurman,BS Knudsen,

For similar investigative techniques: clinical laboratory techniques: culture techniques: tissue culture techniques research abstracts see: investigative techniques: clinical laboratory techniques: culture techniques: tissue culture techniques research

PUBMED ID PMID:

MEDLINE DATE:

Regulation of cell proliferation in a stratified culture system of epithelial cells from prostate tissue. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Cell and tissue research

VOLUME: 325

Page Numbers: 263-76

Journal Abbreviation: Cell Tissue Res.

ISSN: 0302-766X

DAY: 24

MONTH: 03

YEAR: 2006

Regulation of cell proliferation in a stratified culture system of epithelial cells from prostate tissue. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 417625

Regulation of cell proliferation in a stratified culture system of epithelial cells from prostate tissue. Keywords Mesh Terms:

KEYWORDS: Tissue Culture Techniques

MESH TERMS: genetics

Chemical & Substance for Abstract: Regulation of cell proliferation in a stratified culture system of epithelial cells from prostate tissue. Information

Substance Name: Cyclin D1

Registry Number: 136601-57-5

Grant and Affiliation Information for Regulation of cell proliferation in a stratified culture system of epithelial cells from prostate tissue.

AFFILIATION: Clinical Research and Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.

Country: Germany

AGENCY: United States NCI

GRANT: R01CA84069

ACRONYM: CA

MEDLINETA: Cell Tissue Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Regulation of cell proliferation in a stratified culture system of epithelial cells from prostate tissue Related Publications

• Age Modifies the Pituitary TSH Response to Thyroid Failure.
• Bisphenol A facilitates bypass of androgen ablation therapy in prostate cancer.
• Discourses of agency and the search for the authentic self: The case of mood-modifying medicines.
• Gingival epithelial cells heterozygous for Toll-like receptor 4 polymorphisms Asp299Gly and Thr399ile are hypo-responsive to Porphyromonas gingivalis.
• Impact of differential cyclin D1 expression and localisation in prostate cancer.
• Improved parameter estimation for systems with an experimentally located Hopf bifurcation.
• Integrative database management for mouse development: systems and concepts.
• Loss of RB compromises specific heterochromatin modifications and modulates HP1alpha dynamics.
• Migrating with myosin VI.
• Mitogenic action of the androgen receptor sensitizes prostate cancer cells to taxane-based cytotoxic insult.
• Neuroimaging of the serotonin reuptake site requires high-affinity ligands.
• Patterns of organization and management in private and public substance abuse treatment programs.
• Phototoxic furanocoumarins.
• Placental perfusion - a human alternative.
• Psychiatry and torture.
• RB activity alters checkpoint response and chemosensitivity in lung cancer lines.
• RB loss abrogates cell cycle control and genome integrity to promote liver tumorigenesis.
• RB loss promotes aberrant ploidy by deregulating levels and activity of DNA replication factors.
• Regulation of cell proliferation in a stratified culture system of epithelial cells from prostate tissue.
• Regulation of global gene expression in the bone marrow microenvironment by androgen: androgen ablation increases insulin-like growth factor binding protein-5 expression.
• Reprogramming of genetic networks during initiation of the Fetal Alcohol Syndrome.
• Retinoblastoma tumor suppressor status is a critical determinant of therapeutic response in prostate cancer cells.
• SWI/SNF activity is required for the repression of deoxyribonucleotide triphosphate metabolic enzymes via the recruitment of mSin3B.
• Saint Lucia revisited.
• The human placenta - An alternative for studying foetal exposure.
• Tryptophane-205 of human topoisomerase I is essential for camptothecin inhibition of negative but not positive supercoil removal.
• Unique bisphenol A transcriptome in prostate cancer: novel effects on ERbeta expression that correspond to androgen receptor mutation status.
• Variability of estimated binding parameters.
• [Not Available.]
• [Not Available.]