Regulation of cAMP dynamics by Ca2+ and G protein-coupled receptors in the pancreatic -cell: a computational approach.

Regulation of cAMP dynamics by Ca2+ and G protein-coupled receptors in the pancreatic -cell: a computational approach. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Regulation of cAMP dynamics by Ca2+ and G protein-coupled receptors in the pancreatic -cell: a computational approach. Abstract Text:

Leonid E Fridlyand,Mark C Harbeck,Michael W Roe,Louis H Philipson,Leonid E Fridlyand,Mark C Harbeck,Michael W Roe,Louis H Philipson,

In this report we describe a mathematical model for the regulation of cAMP dynamics in pancreatic beta-cells. Incretin hormones such as glucagon-like peptide 1 (GLP-1) increase cAMP and augment insulin secretion in pancreatic beta-cells. Imaging experiments performed in MIN6 insulinoma cells expressing a genetically encoded cAMP biosensor and loaded with fura-2, a calcium indicator, showed that cAMP oscillations are differentially regulated by periodic changes in membrane potential and GLP-1. We modeled the interplay of intracellular calcium (Ca(2+)) and its interaction with calmodulin, G protein-coupled receptor activation, adenylyl cyclases (AC), and phosphodiesterases (PDE). Simulations with the model demonstrate that cAMP oscillations are coupled to cytoplasmic Ca(2+) oscillations in the beta-cell. Slow Ca(2+) oscillations (<1 min(-1)) produce low-frequency cAMP oscillations, and faster Ca(2+) oscillations (>3-4 min(-1)) entrain high-frequency, low-amplitude cAMP oscillations. The model predicts that GLP-1 receptor agonists induce cAMP oscillations in phase with cytoplasmic Ca(2+) oscillations. In contrast, observed antiphasic Ca(2+) and cAMP oscillations can be simulated following combined glucose and tetraethylammonium-induced changes in membrane potential. The model provides additional evidence for a pivotal role for Ca(2+)-dependent AC and PDE activation in coupling of Ca(2+) and cAMP signals. Our results reveal important differences in the effects of glucose/TEA and GLP-1 on cAMP dynamics in MIN6 beta-cells.

Regulation of cAMP dynamics by Ca2+ and G protein-coupled receptors in the pancreatic -cell: a computational approach. Publishing Authors By Initials

LE Fridlyand,MC Harbeck,MW Roe,LH Philipson,LE Fridlyand,MC Harbeck,MW Roe,LH Philipson,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Regulation of cAMP dynamics by Ca2+ and G protein-coupled receptors in the pancreatic -cell: a computational approach. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: American journal of physiology. Cell physiology

VOLUME: 293

Page Numbers: C1924-33

Journal Abbreviation: Am. J. Physiol., Cell Physiol.

ISSN: 0363-6143

DAY: 10

MONTH: 10

YEAR: 2007

Regulation of cAMP dynamics by Ca2+ and G protein-coupled receptors in the pancreatic -cell: a computational approach. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901225

Regulation of cAMP dynamics by Ca2+ and G protein-coupled receptors in the pancreatic -cell: a computational approach. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Regulation of cAMP dynamics by Ca2+ and G protein-coupled receptors in the pancreatic -cell: a computational approach. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Regulation of cAMP dynamics by Ca2+ and G protein-coupled receptors in the pancreatic -cell: a computational approach.

AFFILIATION: Dept. of Medicine, MC-1027, The Univ. of Chicago, 5841 S. Maryland Ave., Chicago, IL 60637. lfridlia@medicine.bsd.uchicago.edu).

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Am J Physiol Cell Physiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Regulation of cAMP dynamics by Ca2+ and G protein-coupled receptors in the pancreatic -cell: a computational approach Related Publications

• 3 year simvastatin treatment and lens nuclear back scattering.
• Action potentials and insulin secretion: new insights into the role of Kv channels.
• Aortic valvular diseases studied by percutaneous thoracic aortography.
• Association between a recently isolated virus and an epidemic of upper respiratory disease in a day nursery.
• C-reactive protein in human colostrum.
• Cold climate genes and the prevalence of type 2 diabetes mellitus.
• Conformational changes of a Ca2+-binding domain of the Na+/Ca2+ exchanger monitored by FRET in transgenic zebrafish heart.
• Cost-effectiveness as a price control.
• Densitometric evaluation at quantitative microradiography.
• Fluorescent Na+-Ca+ exchangers: electrophysiological and optical characterization.
• Homozygous overexpression of the Na+-Ca2+ exchanger in mice: evidence for increased transsarcolemmal Ca2+ fluxes.
• Intermolecular cross-linking of na(+)-ca(2+) exchanger proteins: evidence for dimer formation.
• Kv2.1 ablation alters glucose-induced islet electrical activity, enhancing insulin secretion.
• Localization of sarcolemmal proteins to lipid rafts in the myocardium.
• Microdensitometer system for microradiography.
• Na+/Ca2+ exchanger knockout mice: plasticity of cardiac excitation-contraction coupling.
• OC088: Is there a relationship between visualization of ectopic pregnancy on transvaginal scan and serum quantitative HCG?
• P35.01: Hitting the target but missing the point- the fatal flaw of ectopic pregnancy research.
• Reactive species, cellular repair and risk factors in the onset of type 2 diabetes mellitus: review and hypothesis.
• Regulation of cAMP dynamics by Ca2+ and G protein-coupled receptors in the pancreatic -cell: a computational approach.
• Regulation of cardiac L-type Ca2+ current in Na+-Ca2+ exchanger knockout mice: functional coupling of the Ca2+ channel and the Na+-Ca2+ exchanger.
• Reliability of the drop test for the lacrimal drainage function.
• THE EARLY INTERACTION OF ANIMAL VIRUSES AND CELLS.
• Techniques for surgical retrieval of sperm prior to intra-cytoplasmic sperm injection (ICSI) for azoospermia.
• Techniques for surgical retrieval of sperm prior to intra-cytoplasmic sperm injection (ICSI) for azoospermia.
• The ontogeny of the shoot apex in dicotyledons.
• Treatment of post-irradiation infections in mice. I. The effect of streptomycin and myelopoietic-stimulating substances.
• Virologic aspects on the upper respiratory infections in childhood with special reference to nondiphtheritic croup.
• [In Process Citation]
• [Splitting of some racemic barbitals in optic antipodes. 3. On barbituric acid derivatives]