Regional dissection and determination of loosely bound and non-heme iron in the developing mouse brain.

Regional dissection and determination of loosely bound and non-heme iron in the developing mouse brain. Research Abstract Details 

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Regional dissection and determination of loosely bound and non-heme iron in the developing mouse brain. Abstract Text:

Shino Magaki,Claudius Mueller,Steven M Yellon,James Fox,Joseph Kim,Eugene Snissarenko,Vernon Chin,Manik C Ghosh,Wolff M Kirsch,

Iron is a trace metal essential for normal brain development but toxic in excess as it is capable of generating highly reactive radicals that damage cells and tissue. Iron is stringently regulated by the iron regulatory proteins, IRP1 and IRP2, which regulate proteins involved in iron homeostasis at the posttranscriptional level. In this study, 12 distinct regions were microdissected from the mouse brain and regional changes in the levels of loosely bound and non-heme iron that occur with development were measured. We examined 6, 12, and 24 week old wildtype C57BL/6 mice and mice with a targeted deletion of iron regulatory protein 2 (IRP2-/-) that have been reported to develop neurodegenerative symptoms in adulthood. In wildtype mice, levels of loosely bound iron decreased while non-heme iron increased with development. In contrast, an increase in loosely bound and a more pronounced increase in non-heme iron was seen in IRP2-/- mice between 6 and 12 weeks of age, stemming from lower levels at 6 weeks (the youngest age examined) compared to wildtype. These results have implications for understanding the increase in regional brain iron that is associated with normal aging and is postulated to be exacerbated in neurodegenerative disorders.

Regional dissection and determination of loosely bound and non-heme iron in the developing mouse brain. Publishing Authors By Initials

S Magaki,C Mueller,SM Yellon,J Fox,J Kim,E Snissarenko,V Chin,MC Ghosh,WM Kirsch,

For similar animals: animal population groups: organisms, genetically modified: animals, genetically modified: mice, transgenic: mice, knockout research abstracts see: animals: animal population groups: organisms, genetically modified: animals, genetically modified: mice, transgenic: mice, knockout research

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Regional dissection and determination of loosely bound and non-heme iron in the developing mouse brain. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Brain research

VOLUME: 1158

Page Numbers: 144-50

Journal Abbreviation: Brain Res.

ISSN: 0006-8993

DAY: 8

MONTH: 05

YEAR: 2007

Regional dissection and determination of loosely bound and non-heme iron in the developing mouse brain. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 45503

Regional dissection and determination of loosely bound and non-heme iron in the developing mouse brain. Keywords Mesh Terms:

KEYWORDS: Mice, Knockout

MESH TERMS: deficiency

Chemical & Substance for Abstract: Regional dissection and determination of loosely bound and non-heme iron in the developing mouse brain. Information

Substance Name: Iron Regulatory Protein 2

Registry Number: EC 4.2.1.3

Grant and Affiliation Information for Regional dissection and determination of loosely bound and non-heme iron in the developing mouse brain.

AFFILIATION: Neurosurgery Center for Research, Training and Education, Loma Linda University School of Medicine, Loma Linda, CA 92350, USA.

Country: Netherlands

AGENCY: United States NIA

GRANT: R01 AG020948-05

ACRONYM: AG

MEDLINETA: Brain Res

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