Reduction of anti-HIV-1 Gag immune responses during co-immunization: immune interference by the HIV-1 envelope.

Reduction of anti-HIV-1 Gag immune responses during co-immunization: immune interference by the HIV-1 envelope. Research Abstract Details 

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Reduction of anti-HIV-1 Gag immune responses during co-immunization: immune interference by the HIV-1 envelope. Abstract Text:

Franklin R Toapanta,Jodi K Craigo,Ronald C Montelaro,Ted M Ross,

Immunization with more than one immunogen (co-immunization) is an efficient regimen to induce immunity to multiple antigens. However, immune interference has been reported using multi-plasmid DNA immunizations. HIV-1 envelope (Env) and Gag gene products are the predominant immunogens used in current AIDS vaccines, although, few studies have evaluated possible immune interference when these two antigens are co-administered. Therefore, in this study, immune interference during co-inoculation was examined using DNA vaccines expressing lentiviral Envs and Gag from gene sequences optimized for efficient expression in mammalian cells (codon-optimized). BALB/c mice vaccinated in separate hind legs with each plasmid individually elicited high titer immune responses, however, when HIV-1 Env(gp120) and HIV-1 Gag(p55) DNA plasmids were co-inoculated, there was a reduction in the immune responses elicited to HIV-1 Gag(p55). To determine if the anti-HIV-1 Gag(p55) immune interference was specific to HIV-1 Env(gp120), mice were co-immunized with plasmids expressing the surface envelope protein from two additional lentiviruses, Env(gp130)-SIV or Env(gp90)-EIAV, or a soluble form of hemagglutinin (sHA) from influenza virus and HIV-1 Gag(p55)- or SIV Gag(p55)-DNA. Interestingly, there was no reduction in anti-HIV-1 Gag(p55) immune responses using other lentiviral envelopes or the influenza sHA. Also, none of the lentiviral envelopes reduced anti-SIV Gag(p55) immune responses during co-immunization. Therefore, anti-HIV-1 Gag immune interference appears specific to co-immunizations with HIV-1 Env(gp120) and may involve a yet undefined immunological mechanism(s).

Reduction of anti-HIV-1 Gag immune responses during co-immunization: immune interference by the HIV-1 envelope. Publishing Authors By Initials

FR Toapanta,JK Craigo,RC Montelaro,TM Ross,

For similar proteins: recombinant proteins: vaccines, synthetic: vaccines, dna research abstracts see: proteins: recombinant proteins: vaccines, synthetic: vaccines, dna research

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Reduction of anti-HIV-1 Gag immune responses during co-immunization: immune interference by the HIV-1 envelope. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Current HIV research

VOLUME: 5

Page Numbers: 199-209

Journal Abbreviation: Curr. HIV Res.

ISSN: 1873-4251

DAY: 3

MONTH: Mar

YEAR: 2007

Reduction of anti-HIV-1 Gag immune responses during co-immunization: immune interference by the HIV-1 envelope. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101156990

Reduction of anti-HIV-1 Gag immune responses during co-immunization: immune interference by the HIV-1 envelope. Keywords Mesh Terms:

KEYWORDS: Vaccines, DNA

MESH TERMS: immunology

Chemical & Substance for Abstract: Reduction of anti-HIV-1 Gag immune responses during co-immunization: immune interference by the HIV-1 envelope. Information

Substance Name: Vaccines, DNA

Registry Number: 0

Grant and Affiliation Information for Reduction of anti-HIV-1 Gag immune responses during co-immunization: immune interference by the HIV-1 envelope.

AFFILIATION: Center for Vaccine Research and Department of Medicine, Division of Infectious Diseases, University of Pittsburgh, School of Medicine, Pittsburgh, Pennsylvania 15261, USA.

Country: Netherlands

AGENCY: United States NIAID

GRANT: AI49061

ACRONYM: AI

MEDLINETA: Curr HIV Res

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