Reduced thioredoxin increases proinflammatory cytokines and neutrophil influx in rat airways: modulation by airway mucus.

Reduced thioredoxin increases proinflammatory cytokines and neutrophil influx in rat airways: modulation by airway mucus. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Reduced thioredoxin increases proinflammatory cytokines and neutrophil influx in rat airways: modulation by airway mucus. Abstract Text:

Raymond C Rancourt,Rees L Lee,Heidi O'Neill,Frank J Accurso,Carl W White,

Thioredoxin (Trx) decreases viscosity of cystic fibrosis (CF) sputum. In this study reduced Trx increased the solubility and decreased the size of MUC5B glycoprotein while reducing disulfide bonds in sputum. Because Trx used as a mucolytic would enter airways, this study determined the effects of intratracheal instillation of reduced recombinant human thioredoxin (rhTrx) in naïve rat airways. Reduced rhTrx increased neutrophils and the cytokines TNFalpha, CINC2beta, and MIP3alpha in airways after 4 h. The effect of rhTrx was concentration-dependent. Exposure to saline, human serum albumin, or oxidized rhTrx at equal molarities did not increase airway neutrophils or cytokines. Instilling CF sputum (50 microl) into the lung before reduced rhTrx delivery attenuated these responses. This suggests that rhTrx reduces disulfide bonds present in CF sputum, limiting the reduction of other lung constituents. Together these findings indicate that the chemotactic and cytokine responses are due to the reducing potential of rhTrx and that the potential for inflammation in non-CF and CF patients given aerosolized rhTrx may differ. In parallel studies, increased amounts of the p65 subunit of NF-kappaB were present in nuclear extracts from rat lungs administered reduced rhTrx, suggesting a role for NF-kappaB in these proinflammatory responses.

Reduced thioredoxin increases proinflammatory cytokines and neutrophil influx in rat airways: modulation by airway mucus. Publishing Authors By Initials

RC Rancourt,RL Lee,H O'Neill,FJ Accurso,CW White,

For similar proteins: thioredoxins research abstracts see: proteins: thioredoxins research

PUBMED ID PMID:

MEDLINE DATE:

Reduced thioredoxin increases proinflammatory cytokines and neutrophil influx in rat airways: modulation by airway mucus. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Free radical biology & medicine

VOLUME: 42

Page Numbers: 1441-53

Journal Abbreviation: Free Radic. Biol. Med.

ISSN: 0891-5849

DAY: 20

MONTH: 02

YEAR: 2007

Reduced thioredoxin increases proinflammatory cytokines and neutrophil influx in rat airways: modulation by airway mucus. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8709159

Reduced thioredoxin increases proinflammatory cytokines and neutrophil influx in rat airways: modulation by airway mucus. Keywords Mesh Terms:

KEYWORDS: Thioredoxins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Reduced thioredoxin increases proinflammatory cytokines and neutrophil influx in rat airways: modulation by airway mucus. Information

Substance Name: Thioredoxin-Disulfide Reductase

Registry Number: EC 1.8.1.9

Grant and Affiliation Information for Reduced thioredoxin increases proinflammatory cytokines and neutrophil influx in rat airways: modulation by airway mucus.

AFFILIATION: Department of Pediatrics, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, CO 80206, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: T32 HL-07670

ACRONYM: HL

MEDLINETA: Free Radic Biol Med

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Reduced thioredoxin increases proinflammatory cytokines and neutrophil influx in rat airways: modulation by airway mucus Related Publications

• A flexible computational framework for detecting, characterizing, and interpreting statistical patterns of epistasis in genetic studies of human disease susceptibility.
• A voltage-sensor water pore.
• Chronic vague abdominal pain in children.
• Cimetidine use and the risk for prostate cancer: results from the VITAL cohort study.
• Do breast cancer risk factors modify the association between hormone therapy and mammographic breast density? (United States).
• Effects of photoperiod and intermittent lighting on reproduction in pheasant hens.
• Exposure to the metabolic inhibitor sodium azide induces stress protein expression and thermotolerance in the nematode Caenorhabditis elegans.
• Extremity trauma research in the United States army.
• Felbamate is a subunit selective modulator of recombinant gamma-aminobutyric acid type A receptors expressed in Xenopus oocytes.
• Harnessing RNAi for Analyses of Ras Signaling and Transformation.
• In vitro maturation and fertilization of swamp buffalo oocytes and their subsequent development.
• Inability to immunize patients with metastatic melanoma using plasmid DNA encoding the gp100 melanoma-melanocyte antigen.
• Inhibitory control in children with phenylketonuria.
• Kinetic mechanism of myosinV-S1 using a new fluorescent ATP analogue.
• Mammographic breast density, dense area, and breast area differences by phase in the menstrual cycle.
• PGI2 opens potassium channels in retinal pericytes by cyclic AMP-stimulated, cross-activation of PKG.
• Parachutes and prophylaxis: they both work!
• Polyol synthesis through hydrocarbon oxidation: de novo synthesis of L-galactose.
• Post-thawing survival of motile ram sperm after isolation by layering on protein columns.
• Prevalence of Borrelia burgdorferi in small mammals in New York state.
• Prospects for sexing mammalian sperm edited by Rupert P. Amman and George E. Seidel, Jr.; 288 pages; Cloth - $32.50, Paper - $22.50. Available from Colorado Associated University Press, 1338 Grandview Avenue, Box 480, University of Colorado, Boulder, Colo
• Ras-Sensitive IMP Modulation of the Raf/MEK/ERK Cascade Through KSR1.
• Relationship between estrone sulfate in plasma and litter size at farrowing for sows and gilts.
• Spontaneous baroreflex sensitivity in young and older people during voluntary and electrically evoked isometric exercise.
• Tc-99m HMPAO SPECT in the differential diagnosis of the dementias with histopathologic confirmation.
• The impact of aryl hydrocarbon receptor signaling on matrix metabolism: implications for development and disease.
• The pharmacokinetics of intravenous artesunate in adults with severe falciparum malaria.
• The preference of tryptophan for membrane interfaces.
• The reciprocal stability of FOXO1 and IRS2 creates a regulatory circuit that controls insulin signaling.
• Using verbal fluency to detect very mild dementia of the Alzheimer type.