Reduced antigenicity of type I collagen and proteoglycans in sclerotic dentin.

Reduced antigenicity of type I collagen and proteoglycans in sclerotic dentin. Research Abstract Details 

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Reduced antigenicity of type I collagen and proteoglycans in sclerotic dentin. Abstract Text:

P Suppa,A Ruggeri,F R Tay,C Prati,M Biasotto,M Falconi,D H Pashley,L Breschi,

Antigenic alterations to the dentin organic matrix may be detected by an immunohistochemical approach. We hypothesized that alterations in the antigenicity of type I collagen and proteoglycans occur in sclerotic dentin under caries lesions. Transverse sections were prepared from carious teeth in the sclerotic zone and normal hard dentin. A double-immunolabeling technique was performed on these sections, with anti-type I collagen and anti-chondroitin 4/6 sulfate monoclonal primary antibodies. We used gold-conjugated secondary antibodies to visualize the distribution of intact collagen fibrils and proteoglycans by high-resolution SEM. For sclerotic dentin, labeling densities were 19.57 +/- 3.01/microm2 for collagen and 9.84 +/- 2.62/microm2 for proteoglycans. For normal hard dentin, values were 35.20 +/- 2.73/microm2 and 17.03 +/- 1.98/microm2, respectively. Distribution of intact collagen fibrils and proteoglycans in sclerotic dentin was significantly lower than in normal hard dentin. Reductions in antigenicity from the organic matrix of sclerotic dentin under caries lesions raise concern about the potential of intrafibrillar remineralization.

Reduced antigenicity of type I collagen and proteoglycans in sclerotic dentin. Publishing Authors By Initials

P Suppa,A Ruggeri,FR Tay,C Prati,M Biasotto,M Falconi,DH Pashley,L Breschi,

For similar carbohydrates: polysaccharides: proteoglycans research abstracts see: carbohydrates: polysaccharides: proteoglycans research

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Reduced antigenicity of type I collagen and proteoglycans in sclerotic dentin. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of dental research

VOLUME: 85

Page Numbers: 133-7

Journal Abbreviation: J. Dent. Res.

ISSN: 0022-0345

DAY: 19

MONTH: Feb

YEAR: 2006

Reduced antigenicity of type I collagen and proteoglycans in sclerotic dentin. Information

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LANGUAGE: eng

NlmUniqueID: 354343

Reduced antigenicity of type I collagen and proteoglycans in sclerotic dentin. Keywords Mesh Terms:

KEYWORDS: Proteoglycans

MESH TERMS: immunology

Chemical & Substance for Abstract: Reduced antigenicity of type I collagen and proteoglycans in sclerotic dentin. Information

Substance Name: Proteoglycans

Registry Number: 0

Grant and Affiliation Information for Reduced antigenicity of type I collagen and proteoglycans in sclerotic dentin.

AFFILIATION: Department of SAU & FAL, University of Bologna, Italy.

Country: United States

AGENCY: United States NIDCR

GRANT: R01 DE014911-01A1

ACRONYM: DE

MEDLINETA: J Dent Res

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