Redox-active nucleic-acid replica for the amplified bioelectrocatalytic detection of viral DNA.

Redox-active nucleic-acid replica for the amplified bioelectrocatalytic detection of viral DNA. Research Abstract Details 

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Redox-active nucleic-acid replica for the amplified bioelectrocatalytic detection of viral DNA. Abstract Text:

Fernando Patolsky,Yossi Weizmann,Itamar Willner,

A new concept for the amplified electrochemical detection of the 7229-base viral DNA of M13phi is developed. A thiolated 27-base nucleic acid (1) is assembled on an Au-electrode. Hybridization between the sensing interface and the M13phi DNA is followed by the polymerase-induced replication of the analyte DNA in the presence of dCTP, dGTP, dATP, and ferrocene-tethered-dUTP (2). The generated redox-active replica mediates electron transfer between the enzyme glucose oxidase (GOx) and the electrode and activates the bioelectrocatalyzed oxidation of glucose. The bioelectrocatalyzed oxidation of glucose provides a biocatalytic amplification path for the formation of the redox-active replica. The electrochemical techniques to follow the replication and the bioelectrocatalytic amplification are differential pulse voltammetry and cyclic voltammetry. The electrical responses from the system relate to the bulk concentration of the M13phi DNA, thus enabling the quantitative analysis of the viral gene.

Redox-active nucleic-acid replica for the amplified bioelectrocatalytic detection of viral DNA. Publishing Authors By Initials

F Patolsky,Y Weizmann,I Willner,

For similar biochemical phenomena, metabolism, and nutrition: metabolism: energy metabolism: oxidation-reduction research abstracts see: biochemical phenomena, metabolism, and nutrition: metabolism: energy metabolism: oxidation-reduction research

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Redox-active nucleic-acid replica for the amplified bioelectrocatalytic detection of viral DNA. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 124

Page Numbers: 770-2

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 6

MONTH: Feb

YEAR: 2002

Redox-active nucleic-acid replica for the amplified bioelectrocatalytic detection of viral DNA. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

Redox-active nucleic-acid replica for the amplified bioelectrocatalytic detection of viral DNA. Keywords Mesh Terms:

KEYWORDS: Oxidation-Reduction

MESH TERMS: metabolism

Chemical & Substance for Abstract: Redox-active nucleic-acid replica for the amplified bioelectrocatalytic detection of viral DNA. Information

Substance Name: Glucose Oxidase

Registry Number: EC 1.1.3.4

Grant and Affiliation Information for Redox-active nucleic-acid replica for the amplified bioelectrocatalytic detection of viral DNA.

AFFILIATION: Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.

Country: United States

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MEDLINETA: J Am Chem Soc

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