Recombinase-mediated cassette exchange reveals the selective use of Gq/G11-dependent and -independent endothelin 1/endothelin type A receptor signaling in pharyngeal arch development.

Recombinase-mediated cassette exchange reveals the selective use of Gq/G11-dependent and -independent endothelin 1/endothelin type A receptor signaling in pharyngeal arch development. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Recombinase-mediated cassette exchange reveals the selective use of Gq/G11-dependent and -independent endothelin 1/endothelin type A receptor signaling in pharyngeal arch development. Abstract Text:

Takahiro Sato,Yumiko Kawamura,Rieko Asai,Tomokazu Amano,Yasunobu Uchijima,Dagmara A Dettlaff-Swiercz,Stefan Offermanns,Yukiko Kurihara,Hiroki Kurihara,

The endothelin (Edn) system comprises three ligands (Edn1, Edn2 and Edn3) and their G-protein-coupled type A (Ednra) and type B (Ednrb) receptors. During embryogenesis, the Edn1/Ednra signaling is thought to regulate the dorsoventral axis patterning of pharyngeal arches via Dlx5/Dlx6 upregulation. To further clarify the underlying mechanism, we have established mice in which gene cassettes can be efficiently knocked-in into the Ednra locus using recombinase-mediated cassette exchange (RMCE) based on the Cre-lox system. The first homologous recombination introducing mutant lox-flanked Neo resulted in homeotic transformation of the lower jaw to an upper jaw, as expected. Subsequent RMCE-mediated knock-in of lacZ targeted its expression to the cranial/cardiac neural crest derivatives as well as in mesoderm-derived head mesenchyme. Knock-in of Ednra cDNA resulted in a complete rescue of craniofacial defects of Ednra-null mutants. By contrast, Ednrb cDNA could not rescue them except for the most distal pharyngeal structures. At early stages, the expression of Dlx5, Dlx6 and their downstream genes was downregulated and apoptotic cells distributed distally in the mandible of Ednrb-knock-in embryos. These results, together with similarity in craniofacial defects between Ednrb-knock-in mice and neural-crest-specific Galpha(q)/Galpha(11)-deficient mice, indicate that the dorsoventral axis patterning of pharyngeal arches is regulated by the Ednra-selective, G(q)/G(11)-dependent signaling, while the formation of the distal pharyngeal region is under the control of a G(q)/G(11)-independent signaling, which can be substituted by Ednrb. This RMCE-mediated knock-in system can serve as a useful tool for studies on gene functions in craniofacial development.

Recombinase-mediated cassette exchange reveals the selective use of Gq/G11-dependent and -independent endothelin 1/endothelin type A receptor signaling in pharyngeal arch development. Publishing Authors By Initials

T Sato,Y Kawamura,R Asai,T Amano,Y Uchijima,DA Dettlaff-Swiercz,S Offermanns,Y Kurihara,H Kurihara,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Recombinase-mediated cassette exchange reveals the selective use of Gq/G11-dependent and -independent endothelin 1/endothelin type A receptor signaling in pharyngeal arch development. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Development (Cambridge, England)

VOLUME: 135

Page Numbers: 755-65

Journal Abbreviation: Development

ISSN: 0950-1991

DAY: 16

MONTH: 01

YEAR: 2008

Recombinase-mediated cassette exchange reveals the selective use of Gq/G11-dependent and -independent endothelin 1/endothelin type A receptor signaling in pharyngeal arch development. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8701744

Recombinase-mediated cassette exchange reveals the selective use of Gq/G11-dependent and -independent endothelin 1/endothelin type A receptor signaling in pharyngeal arch development. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Recombinase-mediated cassette exchange reveals the selective use of Gq/G11-dependent and -independent endothelin 1/endothelin type A receptor signaling in pharyngeal arch development. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Recombinase-mediated cassette exchange reveals the selective use of Gq/G11-dependent and -independent endothelin 1/endothelin type A receptor signaling in pharyngeal arch development.

AFFILIATION: Department of Physiological Chemistry and Metabolism, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Development

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Recombinase-mediated cassette exchange reveals the selective use of Gq/G11-dependent and -independent endothelin 1/endothelin type A receptor signaling in pharyngeal arch development Related Publications

• (2S,2'R)-analogue of LG190178 is a major active isomer.
• A study of water-soluble bronchographic media and their clinical application.
• An exonuclease from brain, purification and properties.
• Change in maximum occlusal force in association with maxillofacial growth.
• Characterization of intracellular ca(2+) transient by the hybrid logistic function in aequorin-injected rabbit and mouse papillary muscles.
• Development of a simple diagnostic method for the glaucoma using ocular Fundus pictures.
• Differential gene expression of bone-related proteins in epithelial and fibroblastic cells derived from human periodontal ligament.
• ENZYMATIC FORMATION OF CATECHOL FROM ANTHRANILIC ACID.
• Effect of neurotrophins on differentiation, calcification and proliferation in cultures of human pulp cells.
• Electroconductive Langmuir-Blodgett films containing a carotenoid amphiphile for sugar recognition.
• Familial Creutzfeldt-Jakob Disease with a codon 200 mutation presenting as thalamic syndrome: diagnosis by single photon emission computed tomography using (99m)Tc-ethyl cysteinate dimer.
• Fracture of silicone tracheal T-tube: a rare complication.
• Hydrogen-bonded macrocluster formation of ethanol on silica surfaces in cyclohexane(1).
• Immunohistochemical characteristics of epithelial cell rests of Malassez during cementum repair.
• Irsogladine maleate counters the interleukin-1beta-induced suppression in gap-junctional intercellular communication but does not affect the interleukin-1beta-induced zonula occludens protein-1 levels in human gingival epithelial cells.
• Kimura disease: diagnostic imaging findings and surgical treatment.
• Maintenance of genomic methylation patterns during preimplantation development requires the somatic form of DNA methyltransferase 1.
• Multiple renal cysts, urinary concentration defects, and pulmonary emphysematous changes in mice lacking TAZ.
• Neuronal NO synthase-derived NO: a novel relaxing factor in myocardium?
• Protective Effects of Bilberry (Vaccinium myrtillus L.) Extract on KBrO3-Induced Kidney Damage in Mice.
• Purification and properties of dimethylsulfoxide reductase containing a molybdenum cofactor from a photodenitrifier, Rhodopseudomonas sphaeroides f.s. denitrificans.
• Recombinase-mediated cassette exchange reveals the selective use of Gq/G11-dependent and -independent endothelin 1/endothelin type A receptor signaling in pharyngeal arch development.
• Studies on the salivary secretion induced by substance P in perfused submandibular gland of rat.
• The effect of extracellular calcium ion on gene expression of bone-related proteins in human pulp cells.
• [A case of pollinosis to grass pollens who presented with dyspnea and successfully treated by rush immunotherapy]
• [Challenge to achieve improvements in nursing administration]
• [Experimental study of renal blood flow and renal function during LVAD assistance]
• [Nursing care needs and nursing shift]
• [Safety of simple interruption of the remaining left superior vena cava during extracorporeal circulation]
• [Toranomon Hospital method of patient classification and staffing]