Recombinant lentivirus-delivered short hairpin RNAs targeted to conserved coxsackievirus sequences protect against viral myocarditis and improve survival rate in an animal model.

Recombinant lentivirus-delivered short hairpin RNAs targeted to conserved coxsackievirus sequences protect against viral myocarditis and improve survival rate in an animal model. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Recombinant lentivirus-delivered short hairpin RNAs targeted to conserved coxsackievirus sequences protect against viral myocarditis and improve survival rate in an animal model. Abstract Text:

Yeon-Jung Kim,Jeonghyun Ahn,Soo-Young Jeung,Dae-Sun Kim,Ha-Na Na,Young-Joo Cho,Soo-Hyeon Yun,Youngmee Jee,Eun-Seok Jeon,Heuiran Lee,Jae-Hwan Nam,Yeon-Jung Kim,Jeonghyun Ahn,Soo-Young Jeung,Dae-Sun Kim,Ha-Na Na,Young-Joo Cho,Soo-Hyeon Yun,Youngmee Jee,Eun-Seok Jeon,Heuiran Lee,Jae-Hwan Nam,

Coxsackieviruses are important human pathogens that induce myocarditis and pancreatitis. However, there are no vaccines or therapeutic reagents for their clinical treatment. Although RNA interference (RNAi)-based approaches to the prevention of viral production have been developed recently, limitations to the in vivo delivery systems and variations in the viral target sequences still hamper the strategy. In this study, to overcome these limitations, we have constructed recombinant lentivirus-delivered short hairpin RNAs (shRNAs) against sequences in highly conserved cis-acting replication element (CRE) within the 2C protein of coxsackievirus B3 (CVB3), designated MET-2C. A recombinant lentivirus, designated Met-2C lenti, was constructed that contains the MET-2C sequence, which acts as a shRNA. Met-2C lenti clearly reduced viral production in CVB3-infected cells in vitro. Moreover, the mice injected intraperitoneally with Met-2C lenti had significant reductions in viral titers, viral myocarditis, and proinflammatory cytokines after challenge with CVB3, compared with those in GFP lenti infected control mice. Moreover, Met-2C lenti improved survival rate compared with that of the GFP lenti infected control group. Therefore, Met-2C lenti is potentially a clinical therapeutic agent for the treatment of viral myocarditis.

Recombinant lentivirus-delivered short hairpin RNAs targeted to conserved coxsackievirus sequences protect against viral myocarditis and improve survival rate in an animal model. Publishing Authors By Initials

YJ Kim,J Ahn,SY Jeung,DS Kim,HN Na,YJ Cho,SH Yun,Y Jee,ES Jeon,H Lee,JH Nam,YJ Kim,J Ahn,SY Jeung,DS Kim,HN Na,YJ Cho,SH Yun,Y Jee,ES Jeon,H Lee,JH Nam,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Recombinant lentivirus-delivered short hairpin RNAs targeted to conserved coxsackievirus sequences protect against viral myocarditis and improve survival rate in an animal model. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Virus genes

VOLUME: 36

Page Numbers: 141-6

Journal Abbreviation: Virus Genes

ISSN: 0920-8569

DAY: 3

MONTH: 01

YEAR: 2008

Recombinant lentivirus-delivered short hairpin RNAs targeted to conserved coxsackievirus sequences protect against viral myocarditis and improve survival rate in an animal model. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8803967

Recombinant lentivirus-delivered short hairpin RNAs targeted to conserved coxsackievirus sequences protect against viral myocarditis and improve survival rate in an animal model. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Recombinant lentivirus-delivered short hairpin RNAs targeted to conserved coxsackievirus sequences protect against viral myocarditis and improve survival rate in an animal model. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Recombinant lentivirus-delivered short hairpin RNAs targeted to conserved coxsackievirus sequences protect against viral myocarditis and improve survival rate in an animal model.

AFFILIATION: Department of Biotechnology, The Catholic University of Korea, 43-1, Yeokgok 2-dong, Wonmi-gu, Bucheon-si, Gyeonggi-do, 420-743, Korea.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Virus Genes

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Recombinant lentivirus-delivered short hairpin RNAs targeted to conserved coxsackievirus sequences protect against viral myocarditis and improve survival rate in an animal model Related Publications

• A GUS/luciferase fusion reporter for plant gene trapping and for assay of promoter activity with luciferin-dependent control of the reporter protein stability.
• A highly efficient non-heme manganese complex in oxygenation reactions.
• A new fluoride selective fluorescent as well as chromogenic chemosensor containing a naphthalene urea derivative.
• Accuracy of WHO case definition for SARS screening [The lead author responds:].
• Axial ligand tuning of a nonheme iron(IV)-oxo unit for hydrogen atom abstraction.
• Characterization of cyclofructans from inulin by Saccharomyces cerevisiae Strain displaying cell-surface cycloinulooligosaccharide fructanotransferase.
• Combined 18F-fluorodeoxyglucose-positron emission tomography and computed tomography as a primary screening method for detecting second primary cancers and distant metastases in patients with head and neck cancer.
• Comment on "New physics contributions to the lifetime difference in D0-D0 mixing".
• Comment on "direct observation of laser filamentation in high-order harmonic generation".
• Constitutive Coexpression of Bacillus Endoxylanase and Trichoderma Endoglucanase Genes in Saccharomyces cerevisiae.
• Dioxygen activation and catalytic aerobic oxidation by a mononuclear nonheme iron(II) complex.
• Effective microorganism fermentation extract (EM-X) attenuates airway hyperreactivity and inflammation through selective inhibition of the TH2 response independently of antioxidant activity.
• Effects of endocrine disrupting chemicals on expression of phospholipid hydroperoxide glutathione peroxidase mRNA in rat testes.
• Expression pattern of sulfated glycoprotein-2 (SGP-2) mRNA in rat testes exposed to endocrine disruptors.
• Galanin is up-regulated in colon adenocarcinoma.
• Ginkgolide C inhibits platelet aggregation in cAMP- and cGMP-dependent manner by activating MMP-9.
• Human health risk assessment of explosives and heavy metals at a military gunnery range.
• Inhibitory effect of ginkgolide B on platelet aggregation in a cAMP- and cGMP-dependent manner by activated MMP-9.
• Intra-couple communication dynamics of HIV risk behavior among injecting drug users and their sexual partners in Northern Vietnam.
• Isolation and characterization of cDNA sequences of L-gulono-gamma-lactone oxidase, a key enzyme for biosynthesis of ascorbic acid, from extant primitive fish groups.
• Molecular characterization and mRNA expression during metal exposure and thermal stress of copper/zinc- and manganese-superoxide dismutases in disk abalone, Haliotis discus discus.
• Multiphoton microscope imaging: the behavior of neural progenitor cells in the rostral migratory stream.
• Nucleolar translocalization of GRA10 of Toxoplasma gondii transfectionally expressed in HeLa cells.
• Oral paclitaxel chemotherapy for brain tumors: Ideal combination treatment of paclitaxel and P-glycoprotein inhibitor.
• Overexpression of a chromatin architecture-controlling AT-hook protein extends leaf longevity and increases the post-harvest storage life of plants.
• Production of Cross-Reactive Peptide Antibodies against Viral Capsid Proteins of Human Enterovirus B to Apply Diagnostic Reagent.
• Role of SARS screening clinic in the ED.
• Scheduling mammography screening for the early detection of breast cancer in Korean women.
• Subacute spinal subarachnoid hematoma after spinal anesthesia that causes mild neurologic deterioration.
• Two stage leaching of activated spent HDS catalyst and solvent extraction of aluminium using organo-phosphinic extractant, Cyanex 272.