Recombinant expression of selectively sulfated proteins in Escherichia coli.

Recombinant expression of selectively sulfated proteins in Escherichia coli. Research Abstract Details 

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Recombinant expression of selectively sulfated proteins in Escherichia coli. Abstract Text:

Although tyrosine sulfation is a post-translational modification widespread across multicellular eukaryotes, its biological functions remain largely unknown. This is in part due to the difficulties of synthesizing selectively sulfated proteins. Here we report the selective incorporation of sulfotyrosine into proteins in bacteria by genetically encoding the modified amino acid in response to the amber nonsense codon TAG. Moreover, we show that this strategy enables direct expression in Escherichia coli of sulfo-hirudin, previously inaccessible through recombinant methods. The affinity of sulfo-hirudin toward human thrombin is enhanced more than tenfold over that of desulfo-hirudin, suggesting that sulfo-hirudin may offer clinical advantages for use as an anticoagulant. This general approach to the biosynthesis of sulfated proteins should facilitate further study and application of tyrosine sulfation.

Recombinant expression of selectively sulfated proteins in Escherichia coli. Publishing Authors By Initials

For similar amino acids, peptides, and proteins: amino acids: amino acids, cyclic: amino acids, aromatic: tyrosine research abstracts see: amino acids, peptides, and proteins: amino acids: amino acids, cyclic: amino acids, aromatic: tyrosine research

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Recombinant expression of selectively sulfated proteins in Escherichia coli. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Nature biotechnology

VOLUME: 24

Page Numbers: 1436-40

Journal Abbreviation: Nat. Biotechnol.

ISSN: 1087-0156

DAY: 29

MONTH: 10

YEAR: 2006

Recombinant expression of selectively sulfated proteins in Escherichia coli. Information

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LANGUAGE: eng

NlmUniqueID: 9604648

Recombinant expression of selectively sulfated proteins in Escherichia coli. Keywords Mesh Terms:

KEYWORDS: Tyrosine

MESH TERMS: metabolism

Chemical & Substance for Abstract: Recombinant expression of selectively sulfated proteins in Escherichia coli. Information

Substance Name: Amino Acyl-tRNA Synthetases

Registry Number: EC 6.1.1.-

Grant and Affiliation Information for Recombinant expression of selectively sulfated proteins in Escherichia coli.

AFFILIATION: Department of Chemistry and Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA. ccliu@scripps.edu

Country: United States

AGENCY: United States NIGMS

GRANT: GM62159

ACRONYM: GM

MEDLINETA: Nat Biotechnol

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