Recombinant bovine zona pellucida glycoproteins ZP3 and ZP4 coexpressed in Sf9 cells form a sperm-binding active hetero-complex.

Recombinant bovine zona pellucida glycoproteins ZP3 and ZP4 coexpressed in Sf9 cells form a sperm-binding active hetero-complex. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Recombinant bovine zona pellucida glycoproteins ZP3 and ZP4 coexpressed in Sf9 cells form a sperm-binding active hetero-complex. Abstract Text:

Saeko Kanai,Naoto Yonezawa,Yuichiro Ishii,Masaru Tanokura,Minoru Nakano,Saeko Kanai,Naoto Yonezawa,Yuichiro Ishii,Masaru Tanokura,Minoru Nakano,

The zona pellucida (ZP) is a transparent envelope that surrounds the mammalian oocyte and mediates species-selective sperm-egg interactions. Porcine and bovine ZPs are composed of the glycoproteins ZP2, ZP3, and ZP4. We previously established an expression system for porcine ZP glycoproteins (ZPGs) using baculovirus in insect Sf9 cells. Here we established a similar method for expression of bovine ZPGs. The recombinant ZPGs were secreted into the medium and purified by metal-chelating column chromatography. A mixture of bovine recombinant ZP3 (rZP3) and rZP4 coexpressed in Sf9 cells exhibited inhibitory activity for bovine sperm-ZP binding similar to that of a native bovine ZPG mixture, whereas neither bovine rZP3 nor rZP4 inhibited binding. An immunoprecipitation assay revealed that the coexpressed rZP3/rZP4 formed a hetero-complex. We examined the functional domain structure of bovine rZP4 by constructing ZP4 mutants lacking the N-terminal domain or lacking both the N-terminal and trefoil domains. When either of these mutant proteins was coexpressed with bovine rZP3, the resulting mixtures exhibited inhibitory activity comparable to that of the bovine rZP3/rZP4 complex. Hetero-complexes of bovine rZP3 and porcine rZP4, or porcine rZP3 and bovine rZP4, also inhibited bovine sperm-ZP binding. Our results demonstrate that the N-terminal and trefoil domains of bovine rZP4 are dispensable for formation of the sperm-binding active bovine rZP3/rZP4 complex and, furthermore, that the molecular interactions between rZP3 and rZP4 are conserved in the bovine and porcine systems.

Recombinant bovine zona pellucida glycoproteins ZP3 and ZP4 coexpressed in Sf9 cells form a sperm-binding active hetero-complex. Publishing Authors By Initials

S Kanai,N Yonezawa,Y Ishii,M Tanokura,M Nakano,S Kanai,N Yonezawa,Y Ishii,M Tanokura,M Nakano,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Recombinant bovine zona pellucida glycoproteins ZP3 and ZP4 coexpressed in Sf9 cells form a sperm-binding active hetero-complex. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The FEBS journal

VOLUME: 274

Page Numbers: 5390-405

Journal Abbreviation: FEBS J.

ISSN: 1742-464X

DAY: 26

MONTH: 09

YEAR: 2007

Recombinant bovine zona pellucida glycoproteins ZP3 and ZP4 coexpressed in Sf9 cells form a sperm-binding active hetero-complex. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101229646

Recombinant bovine zona pellucida glycoproteins ZP3 and ZP4 coexpressed in Sf9 cells form a sperm-binding active hetero-complex. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Recombinant bovine zona pellucida glycoproteins ZP3 and ZP4 coexpressed in Sf9 cells form a sperm-binding active hetero-complex. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Recombinant bovine zona pellucida glycoproteins ZP3 and ZP4 coexpressed in Sf9 cells form a sperm-binding active hetero-complex.

AFFILIATION: Graduate School of Science and Technology, Chiba University, Japan.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: FEBS J

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Recombinant bovine zona pellucida glycoproteins ZP3 and ZP4 coexpressed in Sf9 cells form a sperm-binding active hetero-complex Related Publications

• 1.54-microm TM-mode waveguide optical isolator based on the nonreciprocal-loss phenomenon: device design to reduce insertion loss.
• A method for estimating 3-d nuclear parameters using an image taken from a hepatic histopathological section.
• Adenovirus-mediated calponin h1 gene therapy directed against peritoneal dissemination of ovarian cancer: bifunctional therapeutic effects on peritoneal cell layer and cancer cells.
• Adolescent pregnancy: a comparative study between mothers who use public and private health systems.
• Antireflection microstructures fabricated upon fluorine-doped SiO(2) films.
• Association between promoter polymorphisms of nuclear factor-erythroid 2-related factor 2 gene and peptic ulcer diseases.
• Benomyl resistance of Colletotrichum acutatum is caused by enhanced expression of beta-tubulin 1 gene regulated by putative leucine zipper protein CaBEN1.
• Colestimide lowers plasma glucose levels and increases plasma glucagon-like PEPTIDE-1 (7-36) levels in patients with type 2 diabetes mellitus complicated by hypercholesterolemia.
• Comparison of laser epithelial keratomileusis with and without mitomycin C for wavefront customized surface ablations.
• Constant blood flow reduction in premotor frontal lobe regions in ALS with dementia - a SPECT study with 3D-SSP.
• Correlation between expression of MUC1 core protein and outcome after surgery in mass-forming intrahepatic cholangiocarcinoma.
• Dynamical study of femtosecond-laser-ablated liquid-aluminum nanoparticles using spatiotemporally resolved x-ray-absorption fine-structure spectroscopy.
• Efficient derivation of embryonic stem cells by inhibition of glycogen synthase kinase-3.
• Functional polymorphisms in the promoter region of macrophage migration inhibitory factor and chronic gastritis.
• Genetic polymorphisms of molecules associated with inflammation and immune response in Japanese subjects with functional dyspepsia.
• Infusional 5-fluorouracil and cisplatin as first-line chemotherapy in patients with carcinoma of unknown primary site.
• Multi-drug resistance 1 polymorphism is associated with reduced risk of gastric cancer in the Japanese population.
• Native zona pellucida structure is required for completion of sperm acrosome reaction in porcine fertilization.
• Recombinant bovine zona pellucida glycoproteins ZP3 and ZP4 coexpressed in Sf9 cells form a sperm-binding active hetero-complex.
• Regulation of Respiratory Genes by ResD-ResE Signal Transduction System in Bacillus subtilis.
• Semivectorial H-field analysis of rib waveguides by a modified beam-propagation method based on the generalized Douglas scheme.
• Synthesis and aggregation behavior of meso-sulfinylporphyrins: evaluation of S-chirality effects on the self-organization to S-oxo-tethered cofacial porphyrin dimers.
• The new assessment of left atrial plication for giant left atrium.
• Toenail zinc level and gastric cancer risk in Cali, Colombia.
• Toll-like receptor 2 -196 to 174del polymorphism influences the susceptibility of Japanese people to gastric cancer.
• Two-body Coulomb explosion and hydrogen migration in methanol induced by intense 7 and 21 fs laser pulses.
• [A case of primary lung cancer with cervical tuberculous lymphadenitis]
• [Brain perfusion imaging in amyotrophic lateral sclerosis with dementia]
• [Drug-induced aseptic meningitis]
• [Thrombosis--adverse effect of drugs]