Recombinant bactericidal/permeability-increasing protein rBPI21 protects against pneumococcal disease.

Recombinant bactericidal/permeability-increasing protein rBPI21 protects against pneumococcal disease. Research Abstract Details 

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Recombinant bactericidal/permeability-increasing protein rBPI21 protects against pneumococcal disease. Abstract Text:

Amit Srivastava,Heather Casey,Nathaniel Johnson,Ofer Levy,Richard Malley,

Bactericidal/permeability-increasing (BPI) protein has been shown to play an important role in innate immunity to gram-negative bacteria, by direct microbicidal as well as endotoxin-neutralizing action. Here we examined potential interactions between a recombinant 21-kDa bioactive fragment of BPI, rBPI21, and the gram-positive pathogen Streptococcus pneumoniae. rBPI21 bound to pneumococci and pneumolysin (Ply) in a direct and specific fashion. We observed an enhanced inflammatory response in mouse macrophages when rBPI21 was combined with killed pneumococci or supernatant from overnight growth of pneumococci. In addition, rBPI21 augmented the proapoptotic activity of Ply+ (but not Ply-) pneumococci in TLR4-defective murine macrophages (known to be defective also in their apoptotic response to pneumolysin) in a tumor necrosis factor alpha-dependent manner. rBPI21 also enhanced the association of pneumococci with murine macrophages. In a model of invasive pneumococcal disease in TLR4-defective mice, the intranasal administration of rBPI21 following intranasal inoculation of Ply+ pneumococci both enhanced upper respiratory tract cell apoptosis and prolonged survival. We have thus discovered a novel interaction between pneumococcus and rBPI21, a potent antimicrobial peptide previously considered to target only gram-negative bacteria.

Recombinant bactericidal/permeability-increasing protein rBPI21 protects against pneumococcal disease. Publishing Authors By Initials

A Srivastava,H Casey,N Johnson,O Levy,R Malley,

For similar proteins: membrane proteins: receptors, cell surface: receptors, immunologic: receptors, pattern recognition: toll-like receptors: toll-like receptor 4 research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, immunologic: receptors, pattern recognition: toll-like receptors: toll-like receptor 4 research

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Recombinant bactericidal/permeability-increasing protein rBPI21 protects against pneumococcal disease. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Infection and immunity

VOLUME: 75

Page Numbers: 342-9

Journal Abbreviation: Infect. Immun.

ISSN: 0019-9567

DAY: 13

MONTH: 11

YEAR: 2006

Recombinant bactericidal/permeability-increasing protein rBPI21 protects against pneumococcal disease. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 246127

Recombinant bactericidal/permeability-increasing protein rBPI21 protects against pneumococcal disease. Keywords Mesh Terms:

KEYWORDS: Toll-Like Receptor 4

MESH TERMS: immunology

Chemical & Substance for Abstract: Recombinant bactericidal/permeability-increasing protein rBPI21 protects against pneumococcal disease. Information

Substance Name: plY protein, Streptococcus pneumoniae

Registry Number: 0

Grant and Affiliation Information for Recombinant bactericidal/permeability-increasing protein rBPI21 protects against pneumococcal disease.

AFFILIATION: Division of Infectious Diseases, Department of Medicine, Children's Hospital, Boston, MA 02115, USA.

Country: United States

AGENCY: United States NIAID

GRANT: K08 AI 50583-1

ACRONYM: AI

MEDLINETA: Infect Immun

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