Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks.

Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks. Abstract Text:

Yuri Tsuji,Kenji Watanabe,Kimi Araki,Masanori Shinohara,Yuriko Yamagata,Toshiki Tsurimoto,Fumio Hanaoka,Ken-Ichi Yamamura,Masaru Yamaizumi,Satoshi Tateishi,

Post-replication DNA repair facilitates the resumption of DNA synthesis upon replication fork stalling at DNA damage sites. Despite the importance of RAD18 and polymerase eta (Poleta) for post-replication repair (PRR), the molecular mechanisms by which these factors are recruited to stalled replication forks are not well understood. We present evidence that human RAD18 complexed with RAD6B protein preferentially binds to forked and single-stranded DNA (ssDNA) structures, which are known to be localized at stalled replication forks. The SAP domain of RAD18 (residues 248-282) is crucial for binding of RAD18 complexed with RAD6B to DNA substrates. RAD18 mutated in the SAP domain fails to accumulate at DNA damage sites in vivo and does not guide DNA Poleta to stalled replication forks. The SAP domain is also required for the efficient mono-ubiquitination of PCNA. The SAP domain mutant fails to suppress the ultraviolet (UV)-sensitivity of Rad18-knockout cells. These results suggest that RAD18 complexed with RAD6B is recruited to stalled replication forks via interactions with forked DNA or long ssDNA structures, a process that is required for initiating PRR.

Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks. Publishing Authors By Initials

Y Tsuji,K Watanabe,K Araki,M Shinohara,Y Yamagata,T Tsurimoto,F Hanaoka,K Yamamura,M Yamaizumi,S Tateishi,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Genes to cells : devoted to molecular & cellular m

VOLUME: 13

Page Numbers: 343-54

Journal Abbreviation: Genes Cells

ISSN: 1365-2443

DAY: 26

MONTH: Apr

YEAR: 2008

Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9607379

Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks.

AFFILIATION: Cell Genetics, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Genes Cells

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks Related Publications

• 61-year-old woman presenting with deep coma during treatment for adrenal insufficiency.
• Adult-onset neurological degeneration in a patient with Cockayne syndrome and a null mutation in the CSB gene.
• An exon 8-spliced out transcript of neurofibromatosis 2 gene is constitutively expressed in various human tissues.
• Capabilities of a four-layered feedforward neural network: four layers versus three.
• Estimation of CYP2C19 activity by the omeprazole hydroxylation index at a single point in time after intravenous and oral administration.
• Horizontal saccadic palsy associated with gliosis of the brainstem midline.
• Identification of the time-point which gives a plasma rabeprazole concentration that adequately reflects the area under the concentration-time curve.
• Limited frequency of the CYP2C19*17 allele and its minor role in a Japanese population.
• PET/CT for Monitoring Therapy Response in Breast Cancer Patients with Bone Metastases.
• Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks.
• Subacute myelo-optico-neuropathy: clioquinol intoxication in humans and animals.
• The effect of CYP2C19 genotypes on the pharmacokinetics of warfarin enantiomers.
• The role of cytochrome P2C19 in R-warfarin pharmacokinetics and its interaction with omeprazole.
• Ultrastructure of synovio-cartilage junction in rheumatoid arthritis.
• [A successful arterial switch operation for the transportation of the great arteries with posterior aorta--with special reference to its anatomical features and possibility of the indication of the Jatene procedure]
• [Clinical evaluation for extended surgery of lung cancer involving surrounding structures]
• [Congenital oesophago-bronchial fistula with diverticulum in the adult (author's transl)]
• [Long-term bronchial arterial infusion chemotherapy (author's transl)]
• [Lung cancer with giant polypoid extension into the left atrium--a case report]
• [Pharmacological studies on acetylspiramycin]
• [RAD18 regulates DNA damage tolerance]
• [Study of the association of paranasal sinusitis and bronchitis in school children.]