RecA-independent recombination is efficient but limited by exonucleases.

RecA-independent recombination is efficient but limited by exonucleases. Research Abstract Details 

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RecA-independent recombination is efficient but limited by exonucleases. Abstract Text:

Bethany E Dutra,Vincent A Sutera,Susan T Lovett,

Genetic recombination in bacteria is facilitated by the RecA strand transfer protein and strongly depends on the homology between interacting sequences. RecA-independent recombination is detectable but occurs at extremely low frequencies and is less responsive to the extent of homology. In this article, we show that RecA-independent recombination in Escherichia coli is depressed by the redundant action of single-strand exonucleases. In the absence of multiple single-strand exonucleases, the efficiency of RecA-independent recombination events, involving either gene conversion or crossing-over, is markedly increased to levels rivaling RecA-dependent events. This finding suggests that RecA-independent recombination is not intrinsically inefficient but is limited by single-strand DNA substrate availability. Crossing-over is inhibited by exonucleases ExoI, ExoVII, ExoX, and RecJ, whereas only ExoI and RecJ abort gene-conversion events. In ExoI(-) RecJ(-) strains, gene conversion can be accomplished by transformation of short single-strand DNA oligonucleotides and is more efficient when the oligonucleotide is complementary to the lagging-strand replication template. We propose that E. coli encodes an unknown mechanism for RecA-independent recombination (independent of prophage recombination systems) that is targeted to replication forks. The potential of RecA-independent recombination to mediate exchange at short homologies suggests that it may contribute significantly to genomic change in bacteria, especially in species with reduced cellular exonuclease activity or those that encode DNA protection factors.

RecA-independent recombination is efficient but limited by exonucleases. Publishing Authors By Initials

BE Dutra,VA Sutera,ST Lovett,

For similar genetic processes: recombination, genetic research abstracts see: genetic processes: recombination, genetic research

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RecA-independent recombination is efficient but limited by exonucleases. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 216-21

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 20

MONTH: 12

YEAR: 2006

RecA-independent recombination is efficient but limited by exonucleases. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

RecA-independent recombination is efficient but limited by exonucleases. Keywords Mesh Terms:

KEYWORDS: Recombination, Genetic

MESH TERMS: physiology

Chemical & Substance for Abstract: RecA-independent recombination is efficient but limited by exonucleases. Information

Substance Name: exodeoxyribonuclease VII

Registry Number: EC 3.1.11.6

Grant and Affiliation Information for RecA-independent recombination is efficient but limited by exonucleases.

AFFILIATION: Rosenstiel Basic Medical Sciences Research Center and Department of Biology, Brandeis University, Waltham, MA 02454-9110, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM51753

ACRONYM: GM

MEDLINETA: Proc Natl Acad Sci U S A

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