Real-Time Reverse Transcription-PCR Assay for Comprehensive Detection of Human Rhinoviruses.

Real-Time Reverse Transcription-PCR Assay for Comprehensive Detection of Human Rhinoviruses. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Real-Time Reverse Transcription-PCR Assay for Comprehensive Detection of Human Rhinoviruses. Abstract Text:

Xiaoyan Lu,Brian Holloway,Ryan K Dare,Jane Kuypers,Shigeo Yagi,John V Williams,Caroline B Hall,Dean D Erdman,Xiaoyan Lu,Brian Holloway,Ryan K Dare,Jane Kuypers,Shigeo Yagi,John V Williams,Caroline B Hall,Dean D Erdman,

Human rhinoviruses (HRVs) are important contributors to respiratory disease, but their healthcare burden remains unclear, primarily because of the lack of sensitive, accurate, and convenient means of determining their causal role. To address this, we developed and clinically validated the sensitivity and specificity of a real-time reverse transcription-PCR (RT-PCR) assay targeting the viral 5' noncoding region defined by sequences obtained from all 100 currently recognized HRV prototype strains and 85 recently circulating field isolates. The assay successfully amplified all HRVs tested and could reproducibly detect 50 HRV RNA transcript copies, with a dynamic range of over 7 logs. In contrast, a quantified RNA transcript of human enterovirus 68 (HEV68) that showed the greatest sequence homology to the HRV primers and probe set was not detected below a concentration of 5 x 10(5) copies per reaction. Nucleic acid extracts of 111 coded respiratory specimens that were culture positive for HRV or HEV were tested with the HRV real-time RT-PCR assay and by two independent laboratories that used different in-house HRV/HEV RT-PCR assays. Eighty-seven HRV-culture-positive specimens were correctly identified by the real-time RT-PCR assay, and 4 of the 24 HEV-positive samples were positive for HRV. HRV-specific sequences subsequently were identified in these four specimens, suggesting HRV/HEV coinfection in these patients. The assay was successfully applied in an investigation of a coincidental outbreak of HRV respiratory illness among laboratory staff.

Real-Time Reverse Transcription-PCR Assay for Comprehensive Detection of Human Rhinoviruses. Publishing Authors By Initials

X Lu,B Holloway,RK Dare,J Kuypers,S Yagi,JV Williams,CB Hall,DD Erdman,X Lu,B Holloway,RK Dare,J Kuypers,S Yagi,JV Williams,CB Hall,DD Erdman,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Real-Time Reverse Transcription-PCR Assay for Comprehensive Detection of Human Rhinoviruses. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of clinical microbiology

VOLUME: 46

Page Numbers: 533-9

Journal Abbreviation: J. Clin. Microbiol.

ISSN: 0095-1137

DAY: 5

MONTH: 12

YEAR: 2007

Real-Time Reverse Transcription-PCR Assay for Comprehensive Detection of Human Rhinoviruses. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505564

Real-Time Reverse Transcription-PCR Assay for Comprehensive Detection of Human Rhinoviruses. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Real-Time Reverse Transcription-PCR Assay for Comprehensive Detection of Human Rhinoviruses. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Real-Time Reverse Transcription-PCR Assay for Comprehensive Detection of Human Rhinoviruses.

AFFILIATION: 1600 Clifton Road, N.E., Mailstop G04, Atlanta, GA 30333. dde1@cdc.gov.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Clin Microbiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Real-Time Reverse Transcription-PCR Assay for Comprehensive Detection of Human Rhinoviruses Related Publications

• A difficult airway case with GlideScope-assisted fiberoptic intubation.
• Abomasal infusion of butterfat increases milk fat in lactating dairy cows.
• Daily consumption of a dark chocolate containing flavanols and added sterol esters affects cardiovascular risk factors in a normotensive population with elevated cholesterol.
• Dimethylsulfoxide toxicity for seeds. BNWL-480.
• Effects of flour beetles irradiated as pupae. BNWL-480.
• Evaluation of the Dutch version of the migraine quality of life instrument (MSQOL) and its application in headache coping.
• How biological abnormalities separate CFS from depression.
• Human bocavirus in tonsillar lymphocytes.
• Human coronavirus infections in rural Thailand: a comprehensive study using real-time reverse-transcription polymerase chain reaction assays.
• In reply.
• Localization of peripheral pulmonary nodules for thoracoscopic excision: value of CT-guided wire placement.
• Ontogeny and x-radiation sensitivity of the flour beetle, Tribolium. BNWL-480.
• Pediatric adenomatous polyposis syndromes: an update.
• Principal component and multivariate analysis of milk long-chain fatty acid composition during diet-induced milk fat depression.
• Radiation and DDT effects in flour beetles. BNWL-280.
• Rapid reversal of interleukin-6-dependent epithelial invasion in a mouse model of microbially induced colon carcinoma.
• Real-Time Reverse Transcription-PCR Assay for Comprehensive Detection of Human Rhinoviruses.
• STANDARDS FOR THE PREVENTION OF COMPRESSED AIR ILLNESS.
• Screening pneumonia patients for mimivirus.
• Sensitivity of finite helical axis parameters to temporally varying realistic motion utilizing an idealized knee model.
• Serum testosterone is reduced following short-term phytofluene, lycopene, or tomato powder consumption in F344 rats.
• Staphylococci in cheese: some public health aspects.
• Subdural administration of hydrocortisone in multiple sclerosis: effect of ACTH.
• The effect of nitroglycerin ointment on great saphenous vein targeted venous access site diameter with endovenous laser treatment.
• The quality of microparticulated protein.
• Vitamin A status of alcoholics upon admission and after two weeks of hospitalization.